A5072046074- Tendon Structure and Treatment
- Orthopedic Surgery and Rehabilitation
- Bone fractures and treatments
- Orthopaedic implants and arthroplasty
- Bone Tissue Engineering Materials
- Bone Metabolism and Diseases
- Orthopedic Infections and Treatments
- Shoulder Injury and Treatment
- Bone health and osteoporosis research
- Osteoarthritis Treatment and Mechanisms
- Mesenchymal stem cell research
- Knee injuries and reconstruction techniques
- Spectroscopy Techniques in Biomedical and Chemical Research
- Bone health and treatments
- Virus-based gene therapy research
- Periodontal Regeneration and Treatments
- Shoulder and Clavicle Injuries
- Dental Implant Techniques and Outcomes
- Local Government Finance and Decentralization
- Inflammatory mediators and NSAID effects
- Tissue Engineering and Regenerative Medicine
- Total Knee Arthroplasty Outcomes
- Bone and Joint Diseases
- Lower Extremity Biomechanics and Pathologies
- 3D Printing in Biomedical Research
University of Rochester Medical Center
2016-2025
University of Rochester
2016-2025
Mansoura University
2025
University of Bakhtalruda
2020
John Wiley & Sons (United States)
2020
Clinical Orthopaedics and Related Research
2019
Duke Medical Center
2000-2005
Duke University
2002-2005
Duke University Hospital
2002-2004
University of Cincinnati
1998-2003
Mesenchymal stem cells (MSCs) were isolated from bone marrow of 18 adult New Zealand White rabbits. These culture expanded, suspended in type I collagen gel, and implanted into a surgically induced defect the donor's right patellar tendon. A cell-free gel was an identical control left Repair tissues evaluated biomechanically (n = 13) histomorphometrically 5) at 4 weeks after surgery. Compared to their matched controls, MSC-mediated repair tissue demonstrated significant increases 26% (p <...
Pools of human adipose-derived adult stem (hADAS) cells can exhibit multiple differentiated phenotypes under appropriate in vitro culture conditions. Because adipose tissue is abundant and easily accessible, hADAS offer a promising source for engineering other cell-based therapies. However, it unclear whether individual give rise to or each phenotype arises from subset committed progenitor that exists within heterogeneous population. The goal this study was test the hypothesis single are...
Collagen gels were seeded with rabbit bone marrow-derived mesenchymal stem cells (MSCs) and contracted onto sutures at initial cell densities of 1, 4, 8 million cells/ml. These MSC-collagen composites then implanted into full thickness, length, central defects created in the patellar tendons animals providing cells. autologous repairs compared to natural repair identical on contralateral side. Biomechanical, histological, morphometric analyses performed both tissue types 6, 12, 26 weeks...
Abstract A murine segmental femoral bone graft model was used to show the essential role of donor periosteal progenitor cells in healing. Transplantation live harvested from Rosa 26A mice showed that ∼70% osteogenesis on attributed expansion and differentiation cells. Furthermore, engraftment BMP-2-producing marrow stromal nonvital allografts marked increases cortical incorporation neovascularization, suggesting gene-enhanced, tissue engineered functional periosteum may improve allograft...
Adipose-derived adult stem cells (ASCs) have the ability to differentiate into a chondrogenic phenotype in response specific environmental signals such as growth factors or artificial biomaterial scaffolds. In this study, we examined hypothesis that porous scaffold derived exclusively from articular cartilage can induce chondrogenesis of ASCs. Human ASCs were seeded on scaffolds porcine and cultured standard medium without exogenous factors. Chondrogenesis within was evident by quantitative...
Although Staphylococcus aureus osteomyelitis is considered to be incurable, the major bacterial reservoir in live cortical bone has remained unknown. In addition biofilm bacteria on necrotic tissue and implants, studies have implicated intracellular infection of osteoblasts osteocytes as a mechanism chronic osteomyelitis. Thus, we performed first systematic transmission electron microscopy (TEM) formally define reservoirs S. chronically infected mouse (Balb/c J) long tissue. rare, evidence...
Obesity is a risk factor for osteoarthritis (OA), the greatest cause of disability in US. The impact obesity on OA driven by systemic inflammation, and increased inflammation now understood to be caused gut microbiome dysbiosis. Oligofructose, nondigestible prebiotic fiber, can restore lean microbial community profile context obesity, suggesting potentially novel approach treat obesity. Here, we report that - compared with murine associated loss beneficial Bifidobacteria, while key...
Mesenchymal stem cells (MSCs) were isolated from bone marrow, culture-expanded, and then seeded at 1, 4, 8 million cells/mL onto collagen gel constructs designed to augment tendon repair in vivo. To investigate the effects of seeding density on contraction kinetics cellular morphology, cell/collagen was monitored over time up 72 h culture conditions. Constructs 4 showed no significant differences their gross appearance dimensions throughout process. By contrast, 1 initially contracted more...
Abstract Articular cartilage is an avascular connective tissue that exhibits little intrinsic capacity for repair. exists in a reduced oxygen (∼5%) environment vivo; therefore, tension may be important factor regulates the metabolism of chondrocyte progenitors. A number recent studies have developed engineering approaches promoting repair using undifferentiated progenitor cells seeded on biomaterial scaffolds, but known about how might influence these engineered tissues. Human...
The effects of soluble mediators and medium supplements commonly used to induce chondrogenic differentiation in different cell culture systems were investigated define their dose-response profiles potentially synergistic on the adipose-derived adult stromal (ADAS) cells. Human ADAS cells suspended within alginate beads cultured basal with insulin, transferrin, selenious acid (ITS+) or fetal bovine serum (FBS) treated doses combinations TGF-beta1 (0, 1, 10 ng/mL) dexamethasone 10, 100 nM)....
Tissue engineering is a promising therapeutic approach that uses combinations of implanted cells, biomaterial scaffolds, and biologically active molecules to repair or regenerate damaged diseased tissues. Many diverse increasingly complex approaches are being developed articular cartilage, with the underlying premise cells introduced exogenously play necessary role in success engineered tissue replacements. A major consideration remains this field identification characterization appropriate...
Teriparatide is chondroprotective and chondroregenerative in a mouse model of injury-induced osteoarthritis the knee.
The cellular and molecular events responsible for reduced fracture healing with aging are unknown. Cyclooxygenase 2 (COX-2), the inducible regulator of prostaglandin E(2) (PGE(2)) synthesis, is critical normal bone repair. A femoral repair model was used in mice at either 7-9 or 52-56 wk age, evaluated by imaging, histology, gene expression studies. Aging associated a decreased rate chondrogenesis, formation, callus vascularization, delayed remodeling, altered genes involved remodeling....
Abstract Although osteomyelitis (OM) remains a serious problem in orthopedics, progress has been limited by the absence of an vivo model that can quantify bacterial load, metabolic activity bacteria over time, immunity, and osteolysis. To overcome these obstacles, we developed murine implant‐associated OM which stainless steel pin is coated with Staphylococcus aureus implanted transcortically through tibial metaphysis. X‐ray micro‐CT demonstrated concomitant osteolysis reactive bone...
Patients with chronic inflammatory disorders, such as rheumatoid arthritis, often have osteoporosis due to a combination of Tumor necrosis factor-induced increased bone resorption and reduced formation. To test if TNF inhibits formation by affecting the commitment differentiation mesenchymal stem cells (MSCs) into osteoblasts, we examined osteogenic potential MSCs from transgenic (TNF-Tg) mice, model arthritis. MSC-enriched were isolated marrow stromal using negative selection anti-CD45...
Microbubble-enhanced, ultrasound-mediated BMP-6 gene delivery to endogenous progenitor cells induces rapid and efficient repair of critical-sized, nonunion bone fractures in mini-pigs.
Surgical implant-associated bone infections (osteomyelitis) have severe clinical and socioeconomic consequences.Treatment of chronic often involves antibiotics given systemically locally to the affected site in poly (methyl methacrylate) (PMMA) cement.Given high antibiotic concentrations required affect bacteria biofilm, local delivery is important achieve doses at infection site.PMMA not suitable locally-deliver some biofilm-specific antibiotics, including rifampin, due interference with...