A5031681383- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Photosynthetic Processes and Mechanisms
- Enzyme Structure and Function
- Glycosylation and Glycoproteins Research
- Bacterial Genetics and Biotechnology
- Metabolomics and Mass Spectrometry Studies
- Analytical Chemistry and Chromatography
- DNA Repair Mechanisms
- Ubiquitin and proteasome pathways
- Antimicrobial Resistance in Staphylococcus
- DNA and Nucleic Acid Chemistry
- Trace Elements in Health
- Molecular Biology Techniques and Applications
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Protein Structure and Dynamics
- Peptidase Inhibition and Analysis
- Clostridium difficile and Clostridium perfringens research
- Cell Adhesion Molecules Research
- Porphyrin Metabolism and Disorders
- Antimicrobial Peptides and Activities
- Biochemical and Molecular Research
- Plant nutrient uptake and metabolism
- Blood Coagulation and Thrombosis Mechanisms
- Monoclonal and Polyclonal Antibodies Research
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2012-2024
Inserm
2011-2024
Université Grenoble Alpes
2012-2024
CEA Grenoble
2012-2024
Centre National de la Recherche Scientifique
1998-2024
In-Q-Tel
2019
Laboratoire Biologie à Grande Échelle
2011-2017
Institut de Recherches en Technologies et Sciences pour le Vivant
2006-2016
Université Joseph Fourier
2000-2014
Caprion (Canada)
2011
Carbon catabolite repression (CCR) of several Bacillus subtilis catabolic genes is mediated by ATP-dependent phosphorylation histidine-containing protein (HPr), a phosphocarrier the phosphoenolpyruvate (PEP): sugar phosphotransferase system. In this study, we report discovery new B. gene encoding HPr-like protein, Crh (for HPr), composed 85 amino acids. exhibits 45% sequence identity with HPr, but active site His-15 HPr replaced glutamine in Crh. therefore not phosphorylated PEP and enzyme...
Fatty acid and lipoic biosynthesis were investigated in plant mitochondria. Although the mitochondria lack acetyl-CoA carboxylase, our experiments reveal that they contain enzymatic equipment necessary to transform malonate into two main building units for fatty synthesis: malonyl- acetyl-acyl carrier protein (ACP). We demonstrated, by a new method based on complementary use of high performance liquid chromatography mass spectrometry, soluble mitochondrial fatty-acid synthase produces mainly...
To expand the functional analysis of plant mitochondria, we have undertaken building proteome pea mitochondria purified from leaves (green and etiolated), roots seeds. In first stage, focused our proteomic exploration on soluble protein complement green leaf mitochondria. We used traditional two-dimensional polyacrylamide gel electrophoresis, in combination with size exclusion chromatography as a third dimension, to identify major proteins further resolve their macromolecular complexity. The...
In Enterobacter aerogenes , multidrug resistance involves a decrease in outer membrane permeability associated with changes an as yet uncharacterized porin. We purified the major porin from wild‐type strain and resistant strain. characterized this porin, which was found to be OmpC/OmpF‐like protein analysed its pore‐forming properties lipid bilayers. The compared we observed (i) that single‐channel conductance 70% lower than of wild type; (ii) it three times more selective for cations; (iii)...
Azurin * , a by‐product of heterologous expression the gene encoding blue copper protein azurin from Pseudomonas aeruginosa in Escherichia coli was characterized by chemical analysis and electrospray ionization mass spectrometry, its structure determined X‐ray crystallography. It shown that is native with atom replaced zinc metal binding site. Zinc probably incorporated apo‐protein after transport into periplasm. Holo‐azurin can be reconstituted prolonged exposure to high ion concentrations...
Heavy metals are known to generate reactive oxygen species that lead the oxidation and fragmentation of proteins, which become toxic when accumulated in cell. In this study, we investigated role proteasome during cadmium stress leaves Arabidopsis thaliana plants. Using biochemical proteomics approaches, present first evidence an active pathway We identified characterized peptidases acting sequentially downstream from animal cells as follows: tripeptidyl-peptidase II, thimet oligopeptidase,...
Absolute protein quantification, i.e. determining concentrations in biological samples, is essential to our understanding of and physiopathological phenomena. Protein quantification methods based on the use antibodies are very effective widely used. However, over last ten years, absolute by mass spectrometry has attracted considerable interest, particularly for study systems biology as part biomarker development. This interest mainly linked high multiplexing capacity MS analysis,...
The invasion strategy of many viruses involves the synthesis viral gene products that mimic functions cellular proteins and thus interfere with key processes. Here we show adenovirus infection is accompanied by an increased ubiquitin-cleaving (deubiquitinating) activity in host cells. Affinity chromatography on ubiquitin aldehyde (Ubal), which was designed to identify deubiquitinating proteases, revealed presence L3 23K proteinase (Avp) eluate from adenovirus-infected This known be necessary...
Development of new biomarkers needs to be significantly accelerated improve diagnostic, prognostic, and toxicity monitoring as well therapeutic follow-up. Biomarker evaluation is the main bottleneck in this development process. Selected Reaction Monitoring (SRM) combined with stable isotope dilution has emerged a promising option speed step, particularly because its multiplexing capacities. However, analytical variabilities upstream sample handling or incomplete trypsin digestion still need...
Oligonucleotides that contain a single modified pyrimidine, i.e., thymine glycol (Tg), 5,6-dihydrothymine (DHT), and 5-hydroxycytosine (5-OHC) were synthesized in order to investigate the substrate specificity excision mechanism of two Escherichia coli repair enzymes: endonuclease III formamidopyrimidine DNA glycosylase (Fpg). Three techniques analysis employed. A gas chromatography−mass spectrometry (GC−MS) assay with HPLC prepurification was used quantify release bases, while...
The glpK genes of Enterococcus casseliflavus and faecalis, encoding glycerol kinase, the key enzyme uptake metabolism in bacteria, have been cloned sequenced. translated amino acid sequences exhibit strong homology to other bacterial kinases. After expression enterococcalglpK Escherichia coli, both kinases were purified found be phosphorylated by I histidine-containing protein phosphoenolpyruvate:glycose phosphotransferase system. Phosphoenolpyruvate-dependent phosphorylation caused a 9-fold...
To comply with current proteomics guidelines, it is often necessary to analyze the same peptide samples several times. Between analyses, sample must be stored in such a way as conserve its intrinsic properties, without losing either peptides or signal intensity. This article describes two studies designed define optimal storage conditions for between analyses. With use of label-free strategy, conservation was compared over 28-day period three different recipients: standard plastic tubes,...
Aurora kinases are key effectors of mitosis. Plant Auroras functionally divided into two clades. The alpha (Aurora1 and Aurora2) associate with the spindle cell plate implicated in controlling formative divisions throughout plant development. beta (Aurora3) localizes to centromeres likely functions chromosome separation. In contrast wealth data available on role other kingdoms, knowledge their function plants is merely emerging. This exemplified by fact that only histone H3 homolog TPX2 have...
Oxidative reactions within DNA commonly result in base modifications. Among the four bases, guanine is most susceptible to various oxidants, and its related oxidized form, 8-oxo-7,8-dihydroguanine, has been extensively studied terms of repair mutagenicity. However, 8-oxo-7,8-dihydroguanine readily subjected further oxidation, this become a point interest. We recently found that singlet oxygen oxidation led predominant formation oxaluric acid as final product. report herein on biological...
In the framework of C-HPP, our Franco-Swiss consortium has adopted chromosomes 2 and 14, coding for a total 382 missing proteins (proteins which evidence is lacking at protein level). Over last 4 years, French proteomics infrastructure collected high-quality data sets from 40 human samples, including series rarely studied cell lines, tissue types, sample preparations. Here we described step-by-step strategy based on use bioinformatics screening subsequent mass spectrometry (MS)-based...
A proteomics assay was set up to analyze food substrates for eight toxins of the CBRN (chemical, biological, radiological and nuclear) threat, namely ricin, Clostridium perfringens epsilon toxin (ETX), Staphylococcus aureus enterotoxins (SEA, SEB SED), shigatoxins from Shigella dysenteriae entero‐hemorragic Escherichia coli strains (STX1 STX2) Campylobacter jejuni cytolethal distending (CDT). The developed based on an antibody‐free sample preparation followed by bottom‐up LC‐MS/MS analysis...
The Ca2+-dependent interaction between complement serine proteases C1r and C1s is mediated by their α regions, encompassing the major part of N-terminal CUB-EGF-CUB (where EGF epidermal growth factor) module array. In order to define boundaries domain(s) responsible for Ca2+ binding with assess contribution individual modules these functions, CUB, EGF, CUB-EGF fragments were expressed in eucaryotic systems or synthesized chemically. Gel filtration studies, as well measurements intrinsic Tyr...