A5060412693- Metabolomics and Mass Spectrometry Studies
- Toxin Mechanisms and Immunotoxins
- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Alzheimer's disease research and treatments
- Bacillus and Francisella bacterial research
- HIV/AIDS drug development and treatment
- Transgenic Plants and Applications
- Monoclonal and Polyclonal Antibodies Research
- HIV Research and Treatment
- Protein purification and stability
- Biosimilars and Bioanalytical Methods
- Microbial Inactivation Methods
- Biosensors and Analytical Detection
- Bacterial Identification and Susceptibility Testing
- Analytical Chemistry and Chromatography
- Plant tissue culture and regeneration
- Bacteriophages and microbial interactions
- Antimicrobial Resistance in Staphylococcus
- Chemical Synthesis and Analysis
- Yersinia bacterium, plague, ectoparasites research
- Biochemical and Molecular Research
- Apelin-related biomedical research
- Neurological disorders and treatments
- Bacterial Genetics and Biotechnology
CEA Paris-Saclay
2014-2025
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2015-2025
Technologies pour la Santé
2019-2025
Université Paris-Saclay
2017-2025
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2021-2025
Institut de Biologie et Technologies
2013-2023
Metabo (Switzerland)
2023
Service de la Santé Publique
2002-2022
Direction de la Recherche Fondamentale
2021
Unité de Technologies Chimiques et Biologiques pour la Santé
2021
Abstract Background Cerebrospinal fluid biomarker profiles characterized by decreased amyloid-beta peptide levels and increased total phosphorylated tau at threonine 181 (pT181) are currently used to discriminate between Alzheimer’s disease other neurodegenerative diseases. However, these changes not entirely specific disease, it is noteworthy that isoforms of tau, possibly more for the process, have been described in brain parenchyma patients. The precise detection biological fluids remains...
In this study, we show that liquid chromatography coupled with tandem mass spectrometry provides a sensitive, specific, and accurate absolute quantification of Erbitux, human:murine chimeric mAb used for the treatment colorectal cancer. Micrometric magnetized beads, functionalized soluble epidermal growth factor receptor (sEGFR), pharmacological target were specific immunocapture Erbitux allowing assessment antibody's biological potency sample purification. Following digestion trypsin,...
Microtubule-associated Tau proteins are major actors in neurological disorders, the so-called tauopathies. In some of them, and specifically Alzheimer's disease (AD), hyperphosphorylated forms aggregate into neurofibrillary tangles. Following understanding complexity Tau's molecular profile with its multiple isoforms post-translational modifications represent an important issue, a analytical challenge. Immunodetection methods are, fact, limited by number, specificity, sensitivity, capturing...
Tau protein plays a major role in neurodegenerative disorders, appears to be central biomarker of neuronal injury cerebrospinal fluid (CSF), and is promising target for Alzheimer's disease immunotherapies. To quantify tau at high sensitivity gain insights into its naturally occurring structural variations human CSF, we coupled absolute quantification using standard with the multiplex detection capability targeted high-resolution mass spectrometry (MS) on Quadrupole-Orbitrap instrument. Using...
Abstract Biomarkers in sepsis for severity, prediction of outcome or reversibility organ dysfunction are warranted. Measurements plasma DAMP levels at admission can reflect the severity cellular damage septic shock, which might predict prognosis and reduce risk overtreating patients with costly therapies. We measured two DAMPs, S100A8/S100A9 S100A12 during first 24 h shock patients. Forty-nine a similar SOFA scores were selected from our database to compare proportion survivors...
In March 2023, 34 associated cases of iatrogenic botulism were detected in Germany (30 cases), Switzerland (two Austria (one case), and France case). An alert was rapidly disseminated via European Union networks communication platforms (Food- Waterborne Diseases Zoonoses Network, EpiPulse, Early Warning Response System) the International Health Regulation mechanism; outbreak investigated a collaboration. We traced sources to treatment weight loss Türkiye, involving intragastric injections...
The toxin ricin is a biological weapon that may be used for bioterrorist purposes. As member of the group ribosome-inactivating proteins (RIPs), has an A-chain possessing N-glycosidase activity which irreversibly inhibits protein synthesis. In this paper, we demonstrate provided appropriate sample preparation used, enzymatic can exploited functional detection with sensitivity similar to best ELISA and specificity allowing application environmental samples. Ricin first captured by monoclonal...
Apelin peptides were recently identified as endogenous ligands of the APJ receptor. It has been hypothesized that these are initially provided to newborn by nursing and might be involved in gastrointestinal tract development. As apelin may have different effects on receptor a function their size, knowledge exact structure early milk is essential clarify action Bovine colostrum thought contain high concentrations wide diversity peptides, but none them yet rigorously characterized. To identify...
The development of rapid methods for unambiguous identification and precise quantification protein toxins in various matrices is essential public health surveillance. Nowadays, analytical strategies classically rely on sensitive immunological assays, but mass spectrometry constitutes an attractive complementary approach thanks to direct measurement characterization ability. We developed here innovative multiplex immuno-LC-MS/MS method the simultaneous specific three potential biological...
Administration of first-in-class anti-EGFR monoclonal antibody cetuximab is contingent upon extensive pharmacogenomic testing. However in addition to tumor genomics, drug exposure levels could play a critical, yet largely underestimated role, because several reports have demonstrated that pharmacokinetic parameters, particular clearance values, were associated with survival patients. Here, we developed an original bioanalytical method based the use LC-MS/MS technology and simplified sample...
Abstract There is still a need for direct determination (i.e. without dephosphorylation) of nucleoside reverse transcriptase inhibitor (NRTI) triphosphorylated nucleotides in the peripheral‐blood mononuclear cells (PBMCs) HIV‐positive patients. The objective this paper was first to improve our previously described liquid chromatography/tandem mass spectrometry (LC/MS/MS) assay stavudine triphosphate (d4T‐TP). Preparation PBMCs modified reduce degradation d4T‐TP during cell preparation and...
Objectives To investigate whether P-glycoprotein (P-gp) and multidrug resistance proteins (MRPs), which limit the bioavailability of HIV protease inhibitors (PIs) nucleoside reverse transcriptase (NRTIs), modulate anti-HIV activity NRTIs, non-NRTIs PIs in vitro. Design: We used primary cultures major target cells: human monocyte-derived macrophages (MDMs) lymphocytes. Methods P-gp MRP expression response to long-term zidovudine (3′-azido-3′-deoxythymidine; AZT) or indinavir treatment was...
Ricin, the toxin component of Ricinus communis is considered as a potential chemical weapon. Several complementary techniques are required to confirm its presence in environmental samples. Here, we report method combining immunocapture and analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for accurate detection different species R. communis. Liquid samples were applied magnetic particles coated with monoclonal antibody directed against...
We have developed and describe here for the first time a highly sensitive method fast unambiguous detection of viable Escherichia coli in food matrices. The new approach is based on using label-free phages (T4), obligate parasites bacteria, which are attractive pathogen because their inherent natural specificity ease use. A specific immunomagnetic separation was used to capture progeny produced. Subsequently, T4 phage markers were detected by liquid chromatography coupled targeted mass...
Alternative methods to RT-PCR for SARS-CoV-2 detection are investigated provide complementary data on viral proteins, increase the number of tests performed, or identify false positive/negative results. Here, we have developed a simple mass spectrometry assay in nasopharyngeal swab samples using common laboratory reagents. The method employs high sensitivity and selectivity targeted detection, monitoring nine constitutive peptides representative three main proteins straightforward pellet...
Staphylococcal food poisoning outbreaks are caused by the ingestion of contaminated with staphylococcal enterotoxins (SEs). Among 27 SEs described in literature to date, only a few can be detected using immuno-enzymatic-based methods that strongly dependent on availability antibodies. Liquid chromatography, coupled high-resolution mass spectrometry (LC-HRMS), has, therefore, been put forward as relevant complementary method, but for detection limited number enterotoxins. In this work,...
To determine the concentrations of intracellular active anabolites stavudine (d4T) and didanosine (DDI) their interpatient variability in HIV-infected patients to explore relationships between plasma forms.This pilot study included 28 antiretroviral-naive who received d4T (40/30 mg twice daily), ddI (400/250 daily) efavirenz (600 daily). After 6 months therapy, 7 ml blood was collected 0.5 16.2 h 2.5 28.5 after last dose ddI, respectively. Plasma samples were obtained for determination...
Abstract The objective was to develop and validate a routine assay for active intracellular anabolites of stavudine (d4T), nucleoside reverse transcriptase inhibitor in human PBMC, applicable pharmacokinetic studies treatment monitoring. This achieved using liquid chromatography coupled tandem mass spectrometry (LC/MS/MS), which theoretically allies optimum sensitivity, specificity high sample throughput. After cellular lysis Tris/methanol buffer, the extract spiked with 2 [H 8 ]‐ATP...
LC/ESI-MS/MS is a promising alternative to immunoassays in improving the analysis of recombinant therapeutic proteins biological fluids for toxicity and pharmacokinetics purposes. To assess sensitivity validation issues associated with this technique, we use here as model EPI-hNE4, 56-amino acid protein, demonstrate that method based on tandem mass spectrometry combined liquid chromatography electrospray interface can reach similar ELISA but without its potential cross-reactivity. For...