A5111915407- Birth, Development, and Health
- Hormonal and reproductive studies
- Hormonal Regulation and Hypertension
- Pregnancy and preeclampsia studies
- Neonatal Respiratory Health Research
- Sexual Differentiation and Disorders
- Stress Responses and Cortisol
- Estrogen and related hormone effects
- Hypothalamic control of reproductive hormones
- Adrenal Hormones and Disorders
- Growth Hormone and Insulin-like Growth Factors
- Ovarian function and disorders
- Neuropeptides and Animal Physiology
- Regulation of Appetite and Obesity
- Reproductive Physiology in Livestock
- Gestational Diabetes Research and Management
- Congenital Diaphragmatic Hernia Studies
- Adipose Tissue and Metabolism
- Reproductive System and Pregnancy
- Biochemical effects in animals
- Metabolism and Genetic Disorders
- Pharmacogenetics and Drug Metabolism
- Cancer, Hypoxia, and Metabolism
- Biochemical Analysis and Sensing Techniques
- Sperm and Testicular Function
Northwestern University
2009-2024
University of Alabama at Birmingham
2005-2015
The University of Texas Health Science Center at Houston
1979-2015
The University of Texas System
1977-2015
University of Texas Health Science Center at Dallas
1978-2015
The University of Texas Southwestern Medical Center
1982-2009
University of Alabama
1988-2009
Institute for Behavioral Medicine
2006
Johns Hopkins Medicine
1991
Johns Hopkins University
1990-1991
Age-related changes in Leydig cell number, daily sperm production, and circulating hormone levels were studied 15 men between 20 48 yr of age 50 76 age. Testes blood samples obtained at autopsy less than h after death due to trauma or heart attack. number was determined by quantitative histometric estimation the proportion glutaraldehyde-perfused, decapsulated testicular parenchyma occupied nuclei measured average volume both testes each subject. Daily production phase contrast cytometry...
Alternative splicing of the human glucocorticoid receptor (hGR) primary transcript produces two highly homologous protein isoforms, termed hGRα and hGRβ, that differ at their carboxy-termini. In contrast to well characterized isoform, which modulates gene expression in a hormone-dependent fashion, biological significance hGRβ has only recently begun emerge. We others have shown messenger RNA is widely expressed tissues functions as dominant negative inhibitor transfected cells....
The effects of burn trauma in men on the production adrenal and testicular steroids was investigated. Whereas there were significant increases serum cortisol levels urinary 17-hydroxycorticosteroid excretion soon after thermal injury, decreases dehydroepiandrosterone sulfate, dehydroepiandrosterone, androstenedione, testosterone concentrations during first 4 weeks following trauma. Serum androstenediol sulfate also reduced, though insignificantly, 10-23 days postburn. LH unchanged postburn...
Whereas aging has been shown to be associated with striking reductions in circulating levels of adrenal androgens humans, the alteration function that occurs not identified. We sought determine if there are changes zonation men by performing histomorphologic analyses specimens had obtained at autopsy following sudden death due trauma. evaluated adrenals from 21 young (20–29 yrs) and 12 older (54–90 yrs); inclusion criteria required presence medullary tissue specimen fixation within first 24...
Hauth, John C.; Parker, C. Richard Jr.; MacDonald, Paul Porter, Johnston, M. Author Information
During the third trimester of human pregnancy concentrations deoxycorticosterone (DOC) in maternal plasma are 4-50 times those nonpregnant women and men. It has been suggested that increased amount DOC originates fetal compartment. We considered an alternate explanation for high levels or near-term pregnant women, viz., may be derived part from 21-hydroxylation plama progesterone. To test this hyposthesis we measured fractional conversion progesterone to relationship between 3H:14C ratio...
A relationship between sex steroids and the somatomedins (Sms) is well known, but poorly defined. In some primates, including man, there are pubertal increases in Sms, concurrent with increased growth steroid production. current studies, indices of somatic [body weight, crown-rump length (CRL), testis size (testicular volume index)] circulating concentrations testosterone (T), estradiol (E2), dehydroepiandrosterone sulfate (DHEA-S), cortisol, Sm-C were determined (n x003D; 208) 86 male...
In an effort to more completely define the hormonal events associated with onset of puberty in female rat, serum levels a number steroids, cytosol estradiol receptors anterior pituitary-hypothalamic complex, and pituitary responsiveness exogenous LH-RH were quantified. We found that increase (P<0.01) blood concentrations estradiol, progesterone testosterone preceded vaginal opening initial ovulation Holtzman rats. Serum 17-hydroxyprogesterone Δ4-androstenedione not significantly increased...
Human and ovine pregnancies are associated with increases in plasma levels of estrogens angiotensin II (ANG II), cardiac output (CO), blood volume (BV), uterine flow (UBF), as well attenuated ANG pressor responses. We hypothesized that, nonpregnant animals, prolonged estradiol-17 beta (E2 beta) treatment would reproduce these endocrine hemodynamic alterations. Nonpregnant ovariectomized ewes (n = 5) received 5 microgram E2 beta/kg iv followed by 220 micrograms/day for 14 days. Plasma...
Androgens are an integral part of human physiology. The de novo production androgens is generally limited to the adrenal cortex and gonads. Androgen synthesis by these steroidogenic tissues requires bifunctional enzyme cytochrome P450c17, which catalyzes both 17 hydroxylase 17,20 lyase activities. 17,20-lyase activity relevant regulation androgen production, allosterically modulated through action accessory protein, b5 (CytB5). Our objective was determine cellular localization P450c17 CytB5...
In the present investigation role of serum lipoproteins in fetal adrenal steroidogenesis was evaluated. Human tissue maintained organ culture for 6 days presence or absence ACTH. The medium contained either human (10%, vol/vol) lipoprotein-poor with without low density lipoprotein (LDL), high lipoprotein, very lipoprotein. changed daily and assayed pregnenolone sulfate, dehydroepiandrosterone sulfate (DS), cortisol. ACTH, steroid secretion decreased to negligible rates, regardless medium....
Near term the human fetal adrenals (HFAs) initiate production of cortisol, which promotes organ maturation and acts to increase placental CRH biosynthesis. The objective present study was determine whether directly stimulates both cortisol expression steroidogenic enzymes in HFA-definitive zone cells. stimulated a time- dose-dependent manner, with an effective concentration as low 0.01 nm. In real-time RT-PCR experiments, treatment increased mRNA levels acute regulatory protein each needed...
The adrenal glands are the primary source of minerocorticoids, glucocorticoids, and so-called androgens. Under physiological conditions, cortisol androgen synthesis controlled primarily by ACTH. Although it has been established that ACTH can stimulate steroidogenesis, effects on overall gene expression in human cells have not established. In this study, we defined chronic treatment global cultures both adult (AA) fetal (FA) cells. Microarray analysis indicated 48 h caused 30 AA genes 84 FA...
A striking inverse correlation was found in umbilical cord plasma between the concentrations of dehydroisoandrosterone sulfate and low-density lipoprotein (LDL)-cholesterol but not high-density lipoprotein-cholesterol or very low density lipoprotein-cholesterol. Dehydroisoandrosterone is a major secretory product human fetal adrenal principal precursor placental estrogen production. The data suggest that for LDL-cholesterol are influenced by rate utilization steroidogenesis necessarily...
Abstract Context: Near term, the human fetal adrenal increases production of cortisol and dehydroepiandrosterone sulfate (DHEAS). DHEAS, which acts as substrate for placental estrogen production, induces key changes involved in parturition. Objective: The objective this study was to determine quantitatively effect CRH on mRNA levels enzymes needed DHEAS (steroidogenic acute regulatory protein, CYP11A, CYP17, SULT2A1), receptor (CRH-R) subtype(s) responsible action, CRH-R expression zone (FZ)...
In the present investigation it was found that human fetal adrenal tissue maintained in organ culture secreted appreciable quantities of dehydroisoandrosterone sulfate (DS) and cortisol. Pregnenolone also significant amounts, principally as ester. The highest rate secretion these steroids by occurred when both ACTH whole serum were medium. absence ACTH, steroid low. When replaced lipoprotein-poor serum, steroidogenic response to markedly attenuated but not abolished. On basis findings, is...