A5015432447- Aquaculture disease management and microbiota
- Animal Virus Infections Studies
- Aquaculture Nutrition and Growth
- Bacteriophages and microbial interactions
- Herpesvirus Infections and Treatments
- Fish Ecology and Management Studies
- Physiological and biochemical adaptations
- Toxoplasma gondii Research Studies
- Viral Infections and Vectors
- Virus-based gene therapy research
- Proteoglycans and glycosaminoglycans research
- Identification and Quantification in Food
- TGF-β signaling in diseases
- Myxozoan Parasites in Aquatic Species
- Neonatal Health and Biochemistry
- Invertebrate Immune Response Mechanisms
- Microbial infections and disease research
- Marine Bivalve and Aquaculture Studies
- Reproductive biology and impacts on aquatic species
- Caveolin-1 and cellular processes
- Environmental DNA in Biodiversity Studies
- Bamboo properties and applications
- Cell Adhesion Molecules Research
- Metabolism and Genetic Disorders
- Vector-borne infectious diseases
Teikyo University Chiba Medical Center
2024-2025
Japan Fisheries Research and Education Agency
2013-2024
Tokyo Yamate Medical Center
2020
Kyoto Prefectural University
2018
Shimane University
2013
Technical University of Denmark
2009
National Institute of Infectious Diseases
2007
Gifu University
2007
Cardiff University
2005
Hiroshima University
2003-2004
Transforming growth factor (TGF)-β1 is a key cytokine involved in the pathogenesis of fibrosis many organs, whereas interleukin (IL)-6 plays an important role regulation inflammation. Recent reports demonstrate interaction between two cytokines disease states. We have assessed effect IL-6 on TGF-β1 signaling and defined mechanism by which this occurred. Stimulation Smad-responsive promoter (SBE)4-Lux activity was significantly greater presence than that induced alone. Augmented following...
Transforming growth factor-β1 (TGF-β1) is a key cytokine involved in the pathogenesis of fibrosis many organs. We previously demonstrated renal proximal tubular cells that engagement extracellular polysaccharide hyaluronan with its receptor CD44 attenuated TGF-β1 signaling. In current study we examined potential mechanism by which interaction between (HA) and regulates TGF-β function. Affinity labeling receptors unstimulated majority partitioned into EEA-1-associated non-lipid...
ABSTRACT-Mass mortality among common carp Cyprinus carpio, especially in large-sized fish cultured Lake Kasumigaura, has occurred since early October 2003. The affected swam lethargically near the surface. A histopathological examination revealed hyperplasia and necrosis of epithelial cells gills. DNA koi herpesvirus (KHV) was detected gills kidneys moribund by polymerase chain reaction (PCR) assay. virus could not be isolated from tissues, but inoculation gill homogenate into healthy...
Erythrocytic inclusion body syndrome (EIBS) causes mass mortality in farmed salmonid fish, including the coho salmon, Onchorhynchus kisutchi, and chinook O. tshawytscha. The causative agent of disease is a virus with an icosahedral virion structure, but this has not been characterized at molecular level. In study, we sequenced genome purified from EIBS-affected salmon. 10 dsRNA genomic segments (L1, L2, L3, M1, M2, M3, S1, S2, S3, S4), which closely resembles organization piscine...
Three regions of koi herpesvirus (KHV) genomic DNA were compared for 34 samples from Japan, six Indonesia, two Taiwan, one the Philippines, 13 Netherlands, UK, USA and Israel. The analyzed included known PCR-detection targets (SphI-5, 9/5 thymidine kinase gene). KHVs Asian countries very homogeneous, although variants noted based on a single nucleotide polymorphism. In contrast, seven found in outside Asia, closely related to another, they clearly distinct those Asian. results suggest that...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which is the cause of novel disease 2019 (COVID-19), was first reported in Wuhan, China, and now has spread across world as a global pandemic. The propagation from asymptomatic polymerase chain reaction (PCR)-positive individuals represents complicating factor efforts to control COVID-19 We examined course PCR assays duration viral shedding 23 or mild patients cruise ship who were admitted our hospital. Among these cases, median...
Red sea bream iridoviral disease (RSIVD) causes high economic damage in mariculture Asian countries. However, there is little information on the source of infection and viral dynamics fish farms. In present study, RSIV a farm that mainly reared juveniles broodstocks red (Pagrus major) were monitored over 3 years (2016 to 2018) by targeting environmental DNA (eDNA) seawater. Our monitoring demonstrated iridovirus (RSIV) was detected from eDNA at least 5 days before an RSIVD outbreak...
<title>Abstract</title> <bold>Background</bold> Although an increase in plasma levels of non-esterified fatty acids (NEFA) during hemodialysis (HD) is reported, the precise pathophysiology remains unclear. We hypothesized that elevation NEFA reflects “cell starvation” or sudden-onset catabolism caused primarily by a steep depletion insulin HD session.<bold>Materials and Methods</bold> measured five cases circulating session under conditions without with intradialytic parenteral nutrition...
Herpesviral haematopoietic necrosis has caused great economic damage to goldfish Carassius auratus aquaculture in Japan. The existence of cyprinid herpesvirus 2 (CyHV-2), the causative agent, also been reported from several other countries. To prevent spread areas, basic virological information such as viral kinetics infected fish is essential. Experimental infection trials using reliably prepared CyHV-2 for defining are difficult carry out because successful and sustainable propagation this...
Improvement of a PCR method using the Sph I-5 primer set, which has been used for detection KHV in Japan, was carried out to reduce negative factors, such as appearance non-specific reactions and comparatively long reaction time. Moreover, there wrong sequence original reverse primer. The modified protocol with corrected set is follows : initial denaturation at 94°C 30s, 40 cycles amplification (denaturation annealing 63°C 30s elongation 72°C 30s) final 7min. improved reduced total time...
Red sea bream iridovirus (RSIV) causes substantial economic damage to aquaculture. In the present study, RSIV in wild fish near aquaculture installations was surveyed evaluate risk of being an infection source for outbreaks cultured fish. total, 1102 fish, consisting 44 species, were captured from 2 areas western Japan using fishing, gill nets, and fishing baskets between 2019 2022. Eleven 7 species confirmed harbor genome a probe-based real-time PCR assay. The mean viral load RSIV-positive...
Common carp Cyprinus carpio were experimentally exposed to koi herpesvirus (KHV) under water temperature regimes ranging from 16°C 28°C. Fish maintained at 16°C, 23°C and 28°C died due infection 21-52, 5-20 7-14 days post-virus exposure (dpe), respectively. Cohabitation experiments in which KHV-infected common kept with naive revealed that infectious virus was continuously shed the infected for 34 (7-40 dpe) 14 (1-14 12 (3-14 The relatively extended duration of shedding observed suggests...
Red sea bream iridovirus (RSIV) is a representative of the genus Megalocytivirus which causes severe disease to aquaculture fish, mainly in Japan and South-east Asia. However, information assess viral kinetics RSIV fish limited since reports on experimental infection by immersion route, natural are scarce. In this study, method evaluate titre was first developed. Experimental infections were continuously performed using cell culture as inoculum juvenile Japanese amberjack ( Seriola...
Carp edema virus disease (CEVD), or koi sleepy disease, is caused by CEV. Here, we report the complete genome sequence of CEV strain FTI2020, isolated from carp. This information has great potential for improving our understanding genetic characteristics this piscine poxvirus.
Aquatic animal viruses are considered to be transmitted via environmental water between fish farms. This study aimed understand the actual transmission risk of red sea bream iridovirus (RSIV) through among An DNA (eDNA) method using iron-based flocculation coupled with large-pore filtration was used monitor RSIV copies in seawater from farms and an experimental infection model. dispersion a net pen where disease outbreak occurred visualized by inverse distance weighting multiple-sampling...