A5072974132- Advanced Proteomics Techniques and Applications
- Molecular Sensors and Ion Detection
- Mass Spectrometry Techniques and Applications
- Luminescence and Fluorescent Materials
- Analytical chemistry methods development
- Metabolomics and Mass Spectrometry Studies
- Antimicrobial Resistance in Staphylococcus
- Analytical Chemistry and Sensors
- Electrochemical Analysis and Applications
- Gold and Silver Nanoparticles Synthesis and Applications
- Antibiotic Resistance in Bacteria
- Bacterial Identification and Susceptibility Testing
- Advanced biosensing and bioanalysis techniques
- Advanced Nanomaterials in Catalysis
- Bacterial biofilms and quorum sensing
- Analytical Chemistry and Chromatography
- Nanocluster Synthesis and Applications
- Electrochemical sensors and biosensors
- Nanoparticles: synthesis and applications
- Mercury impact and mitigation studies
- Metal complexes synthesis and properties
- Nanomaterials for catalytic reactions
- Crystallization and Solubility Studies
- Heavy metals in environment
- Bacteriophages and microbial interactions
Universidade de Vigo
2000-2024
Universidade Nova de Lisboa
2015-2024
Rede de Química e Tecnologia
2015-2024
Associação Científica Proteomass
2015-2024
University of Lisbon
2015-2024
Clovis Oncology (United States)
2015-2024
Universidade de Santiago de Compostela
2009-2024
Madan Parque
2023
Laboratório Associado para a Química Verde
2019
Instituto Federal de Educação, Ciência e Tecnologia do Ceará
2018
Mass spectrometry is one of the most important techniques in field proteomics. MALDI-TOF mass has become popular during last decade due to its high speed and sensitivity for detecting proteins peptides. MALDI-TOF-MS can be also used combination with Machine Learning statistical methods knowledge discovery. Although there are many software libraries tools that combined these kind analysis, still a need all-in-one solutions graphical user-friendly interfaces avoiding programming skills....
The interpretation of in vitro cytotoxicity data Cu(II)-1,10-phenanthroline (phen) complexes normally does not take into account the speciation that undergo cell incubation media and its implications cellular uptake mechanisms action. We synthesize test activity several distinct Cu(II)-phen compounds; up to 24 h incubation, cytotoxic differs for Cu corresponding free ligands, but longer times (e.g., 72 h), all compounds display similar activity. Combining use spectroscopic, spectrometric,...
Methicillin-resistant Staphylococcus aureus (MRSA) is one of the main pathogens causing chronic infections, mainly due to its capacity form biofilms. However, mechanisms underlying biofilm formation MRSA strains from different types human infections are not fully understood. isolated distinct were characterized aiming determine their biofilm-forming capacity, resistance conventional antibiotics and prevalence biofilm-related genes, including, icaA, icaB, icaC, icaD, fnbA, fnbB, clfA, clfB,...
A new chemosensor based on Seleno-BODIPY (4) was designed and synthesized for the rapid sensitive detection of CN–, F−, OH– ions in THF, exhibiting ultra-low limits 26.26 nM, 43.05 8.64 respectively, through a turn-on fluorescent process. The seleno-funcionalization step novel methodology developed by our group, allowing direct functionalization BODIPY core. This procedure involves quick reaction utilizing Ph2Se2 as selenium source, BPO (benzoyl peroxide), p-TSA (p-toluenesulfonic acid)...
Proteolytic digestion of proteins in seconds under an ultrasonic field provided by high-intensity focused ultrasound (HIFU) has been achieved. Successful in-solution and in-gel tryptic 60 s or less was demonstrated either MALDI-TOF mass spectrometry liquid chromatography-electrospray ion trap (RP−HPLC−ESI−IT−MS/MS). The efficiency this new procedure for protein compared favorably with those attained using conventional overnight incubation methods. performance the method also specific...
This paper describes the dramatic activity enhancement of two proteolytic enzymes (protease XIV and subtilisin) when treated with an ultrasonic probe their application to total Se determination speciation in biological samples. Total extraction from enriched yeast is performed 10 mg plus 1 protease a sonication time 5 s, whereas 30 s needed for extracting selenomethionine. In both cases, aqueous media was used. spectacular finding important because enzymatic procedure usually requires long...
New pyrazole-porphyrin conjugates were successfully prepared from a reaction of β-porphyrin-chalcone derivatives with phenylhydrazine in acetic acid followed by an oxidative step. This fast and efficient synthetic approach provided the expected compounds yields up to 82%. The sensing ability new porphyrin-pyrazole detect metal ions Ag(+), Na(+), K(+), Mg(2+), Ca(2+), Ni(2+), Cu(2+), Zn(2+), Cd(2+), Hg(2+), Pb(2+), Cr(3+) was studied spectrophotometric spectrofluorimetric titrations. In...
The spatial distribution of chemical elements in different types samples is an important field several research areas such as biology, paleontology or biomedicine, among others. Elemental imaging by laser ablation inductively coupled plasma mass spectrometry (LA–ICP–MS) effective technique for qualitative and quantitative due to its high resolution sensitivity. By applying this technique, vast amounts raw data are generated obtain high-quality images, essentially making the use specific...
Owls are nocturnal predators that inhabit urbanized and farmlands. They in direct contact with other animals, both livestock small wild rodents they mostly feed on. Staphylococci can be commensal pathogenic bacteria widespread across the various ecological niches. We aimed to isolate staphylococci from owls characterize their antimicrobial resistance, virulence factors genetic lineages. Swab samples were collected throat cloaca of 114 admitted two rehabilitation centers Portugal. The...
Coagulase-negative staphylococci are commensals that known to be prevalent in most environments, and they also an important reservoir of antimicrobial-resistant genes. Staphylococcal infections animal husbandry a high economic burden. Thus, we aimed determine the prevalence species diversity methicillin-resistant coagulase-negative (MRCoNS) poultry slaughtered for human consumption study antimicrobial resistance isolates. Swab samples were recovered from 220 commercial chickens, homebred...