A5036989614- CRISPR and Genetic Engineering
- BRCA gene mutations in cancer
- DNA Repair Mechanisms
- Erythrocyte Function and Pathophysiology
- Caveolin-1 and cellular processes
- Cancer Treatment and Pharmacology
- Herpesvirus Infections and Treatments
- Genomics and Rare Diseases
- CAR-T cell therapy research
- Mass Spectrometry Techniques and Applications
- Virus-based gene therapy research
- HIV Research and Treatment
- T-cell and B-cell Immunology
- Cancer-related gene regulation
- Immune Cell Function and Interaction
The Ohio State University
2019-2024
University School
2024
Multiple sclerosis is an autoimmune disease of the central nervous system (CNS) mediated by CD4+ T cells and modeled via experimental encephalomyelitis (EAE). Inhibition PRMT5, major Type II arginine methyltransferase, suppresses pathogenic cell responses EAE. PRMT5 transiently induced in proliferating memory inflammatory Th1 during However, mechanisms driving protein induction repression as expand return to resting currently unknown. Here, we used naive mouse human Th1/Th2 models identify...
In recent years, human serum albumin (HSA) has been characterized as an ideal drug carrier in the cancer arena. Caveolin-1 (Cav-1) established principal structural protein of caveolae and, thus, critical for caveolae-mediated endocytosis. Cav-1 shown to be overexpressed cancers lung and pancreas, among others. We found that expression plays a role both HSA uptake response albumin-based chemotherapies. As such, developing novel chemotherapy is more selective tumors with high or levels...
Single nucleotide variants are the most frequent type of sequence changes detected in genome and these frequently uncertain significance (VUS). VUS DNA for which disease risk association is unknown. Thus, methods that classify functional impact a can be used as evidence variant interpretation. In case breast ovarian cancer specific tumor suppressor protein, BRCA1, pathogenic missense score loss function an assay homology-directed repair (HDR) double-strand breaks. We previously published...
ABSTRACT The abundance of MHC-I on the cell surface depends its association with antigenic peptides transported to endoplasmic reticulum by Transporter Associated Antigen Processing (TAP), a peptide channel composed TAP1 and TAP2. We functionally screened over 1400 TAP2 variants for effects abundance. Amino-acid substitutions loss function (LOF) clustered in NTP-binding domain along TAP1-TAP2 binding interface, suggesting that these interfered TAP conformational changes associated transport....
Delineating functionally normal variants from abnormal in tumor suppressor proteins is critical for cancer surveillance, prognosis, and treatment options. BRCA1 a protein that has many of uncertain significance which are not yet classified as or abnormal. In vitro functional assays can be used to identify the impact variant when been categorized through clinical observation. Here we employ homology-directed repair (HDR) reporter assay evaluate over 300 missense nonsense between amino acid...
Human serum albumin (HSA) improves the pharmacokinetic profile of drugs attached to it, making it an attractive carrier with proven clinical success. In our previous studies, we have shown that Caveolin-1 (Cav-1) and caveolae-mediated endocytosis play important roles in uptake HSA albumin-bound drugs. Doxorubicin is FDA-approved chemotherapeutic agent effective against multiple cancers, but its applicability has been hampered by high toxicity levels. this study, a doxorubicin-prodrug was...
ABSTRACT Herpes Simplex Virus 1 evades the host immune system by expressing a protein, ICP47, that binds to and inhibits heterodimeric Transporter Associated with Antigen Processing (TAP). We screened library of 1786 variants in TAP2, one components TAP heterodimer, identified 39 were resistant inhibition ICP47. Of these variants, five individually tested, three (T257I, S274H, T244R) confirmed be significantly This resistance did not extend Epstein Barr BNLF2a another viral factor known...
Single nucleotide variants are the most frequent type of sequence changes detected in genome and these frequently uncertain significance (VUS). VUS DNA for which disease risk association is unknown. Thus, methods that classify functional impact a can be used as evidence variant interpretation. In case breast ovarian cancer specific tumor suppressor protein, BRCA1, pathogenic missense score loss function an assay homology-directed repair (HDR) double-strand breaks. We previously published...