A5064654896- Insect and Arachnid Ecology and Behavior
- Physiological and biochemical adaptations
- Insect and Pesticide Research
- Plant and animal studies
- Neurobiology and Insect Physiology Research
- Heat shock proteins research
- Insect Resistance and Genetics
- Insect-Plant Interactions and Control
- Insect symbiosis and bacterial influences
- thermodynamics and calorimetric analyses
- Genetics, Aging, and Longevity in Model Organisms
- Animal Behavior and Reproduction
- Insect Pest Control Strategies
- Invertebrate Immune Response Mechanisms
- Insect behavior and control techniques
- Entomopathogenic Microorganisms in Pest Control
- Forest Insect Ecology and Management
- Forest Ecology and Biodiversity Studies
- Greenhouse Technology and Climate Control
- Plant and fungal interactions
- Environmental Toxicology and Ecotoxicology
- Hemiptera Insect Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Ecology and Vegetation Dynamics Studies
- Plant Parasitism and Resistance
Edward T. Schafer Agricultural Research Center
2015-2024
Agricultural Research Service
2014-2024
North Dakota State University
2024
United States Department of Agriculture
2013-2022
Bioscience Research
2007-2022
Laboratoire Physiologie Cellulaire & Végétale
2015
The Ohio State University
1990-2007
East Tennessee State University
1998
Diapause, the dormancy common to overwintering insects, evokes a unique pattern of gene expression. In flesh fly, most, but not all, fly's heat shock proteins (Hsps) are up-regulated. The diapause up-regulated Hsps include two members Hsp70 family, one member Hsp60 family (TCP-1), at least four small Hsp and pseudogene. Expression an cognate, Hsc70 , is uninfluenced by diapause, Hsp90 actually down-regulated during thus differs from stress responses that elicit synchronous up-regulation all...
For bees, many roads lead to social harmony Eusociality, where workers sacrifice their reproductive rights support the colony, has evolved repeatedly and represents most form of evolution in insects. Kapheim et al. looked across genomes 10 bee species with varying degrees sociality determine underlying genomic contributions. No one path led eusociality, but similarities were seen features such as increases gene regulation methylation. It also seems that selection pressures relaxed after...
Relatively little is known about the genomic basis and evolution of wood-feeding in beetles. We undertook genome sequencing annotation, gene expression assays, studies plant cell wall degrading enzymes, other functional comparative Asian longhorned beetle, Anoplophora glabripennis, a globally significant invasive species capable inflicting severe feeding damage on many important tree species. Complementary genes encoding enzymes involved digestion woody tissues or detoxification...
The Colorado potato beetle is one of the most challenging agricultural pests to manage. It has shown a spectacular ability adapt variety solanaceaeous plants and variable climates during its global invasion, and, notably, rapidly evolve insecticide resistance. To examine evidence rapid evolutionary change, understand genetic basis herbivory resistance, we tested for structural functional genomic changes relative other arthropod species using genome sequencing, transcriptomics, community...
ABSTRACT The transcriptional responses of insects to long-term, ecologically relevant temperature stress are poorly understood. Long-term exposure low temperatures, commonly referred as chilling, can lead physiological effects collectively known chill injury. Periodically increasing temperatures during long-term chilling has been shown increase survival in many insects. However, the transcripts responsible for this have never characterized. Here, we present first transcriptome-level analysis...
Although the immediate effects of temperature stress are well documented, longer-term such stresses more poorly known.In these experiments, we investigate suboptimal and supraoptimal temperatures during pharate adult development on fecundity in ¯esh ¯y, Sarcophaga crassipalpis Macquart.A 1 h cold shock at ±10°C red-eye stage decreases both sexes.Induction rapid hardening by pre-treatment 0°C for 2 partially prevents reproductive impairment.Heat adults 45°C also reduces sexes, but inducing...
Several cDNAs isolated from brains of diapausing pupae the flesh fly, Sarcophaga crassipalpis , show expression patterns unique to diapause. To isolate such a diapause pupal brain cDNA library was screened by using an elimination hybridization technique, and that did not hybridize with probes constructed RNA nondiapausing were selected for further screening. The 95 clones in initial screen characterization. These then against nondiapause poly(A) + Northern blots. secondary identified 4...
Abstract Insect diapause (dormancy) synchronizes an insect's life cycle to seasonal changes in the abiotic and biotic resources required for development reproduction. Transcription analysis of post‐diapause quiescent transition alfalfa leafcutting bee Megachile rotundata F abricius identifies 643 up‐regulated gene transcripts 242 down‐regulated transcripts. The log 2 fold change expression levels ranges from −5 7. Transcripts several pivotal diapause‐related processes, including chromatin...
ABSTRACT Social bees have been extensively studied for their gut microbial functions, but the significance of microbiota in solitary remains less explored. Solitary bee, Megachile rotundata females provision offspring with pollen from various plant species, harboring a diverse community that colonizes larvae guts. The Apilactobacillus is most abundant microbe, evidence concerning effects and other microbes on growth survival are lacking. We hypothesized presence abundance would enhance...
Abstract Diapausing pharate first instars of the gypsy moth, Lymantria dispar , respond to high temperature (37–41°C) by suppressing normal protein synthesis and synthesizing a set seven heat shock proteins with M r s 90,000, 75,000, 73,000, 60,000, 42,000, 29,000, 22,000 as determined SDS‐PAGE. During recovery at 25°C from shock, gradually decreases over period 6 h, while is restored. A subset these same also expressed during 4°C or brief exposures low (‐10 20°C), its expression more...
Significance In this study, we characterize the developmental mechanisms shaping body size in solitary bee pollinator, Osmia lignaria . This study manipulates larval development a to understand how shape adult size. We based our approach on insect model that postulates critical weight is necessary for normal metamorphosis. However, identified food absence as cue metamorphosis species, rather than “critical weight.” These data directly challenge ubiquity of weights and offer insights about...
In species that care for their young, provisioning has profound effects on offspring fitness. Provisioning is important in honeybees because nutritional cues determine whether a female becomes reproductive queen or sterile worker. A qualitative difference between the larval diets of queens and workers thought to drive this divergence; however, no single compound seems be responsible. Diet quantity may have role during honeybee caste determination yet never been formally studied. Our goal was...
Insects exposed to constant low temperatures (CLT) exhibit high rates of mortality as well a variety sublethal effects. In many species, interruptions CLT with brief pulses warm (fluctuating thermal regimes, FTR) lead increases in survival and fewer However, we still lack complete understanding the physiological mechanisms activated during FTR. this review, discuss recent advances FTR's underlying molecular mechanisms. We knowledge gaps related potential trade-offs between beneficial effects...