A5102762107- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Chemokine receptors and signaling
- Chemical Synthesis and Analysis
- Chronic Lymphocytic Leukemia Research
- Immunotherapy and Immune Responses
- Antimicrobial Peptides and Activities
- Microbial Metabolic Engineering and Bioproduction
- Mass Spectrometry Techniques and Applications
- Enzyme Catalysis and Immobilization
- Nicotinic Acetylcholine Receptors Study
- Glycosylation and Glycoproteins Research
- Microbial Natural Products and Biosynthesis
- Analytical Chemistry and Chromatography
- RNA and protein synthesis mechanisms
University of Manchester
2017-2020
Czech Academy of Sciences, Institute of Biotechnology
2018-2020
Triazole-capped α-aminoisobutyric acid (Aib) octameric foldamers formed very active ion channels in phospholipid bilayers after the addition of copper(<sc>ii</sc>) chloride, with activity “turned off” by extraction.
Polyketides are a class of specialised metabolites synthesised by both eukaryotes and prokaryotes. These chemically structurally diverse molecules heavily used in the clinic include frontline antimicrobial anticancer drugs such as erythromycin doxorubicin. To replenish clinicians' diminishing arsenal bioactive molecules, promising strategy aims at transferring polyketide biosynthetic pathways from their native producers into biotechnologically desirable host Escherichia coli. This approach...
Helical α-aminoisobutyric acid (Aib) foldamers show great potential as devices for the communication of conformational information across phospholipid bilayers, but determining their conformation in bilayers remains a challenge. In present study, Raman, Raman optical activity (ROA), infrared (IR) and vibrational circular dichroism (VCD) spectroscopies have been used to analyze preferences Aib solution when interacting with bilayers. A 310 -helix marker band at 1665-1668 cm-1 spectra was that...
Abstract CXC chemokine receptor 4 (CXCR4) is overexpressed by a broad range of hematological disorders, and its interaction with ligand 12 (CXCL12) central importance in the retention chemoprotection neoplastic cells bone marrow lymphoid organs. In this article, we describe biological evaluation new CXCR4-targeting -antagonizing molecule (BAT1) that designed show that, when incorporated into liposomal drug delivery system, it can be used to deliver cancer therapeutics at high levels chronic...
Abstract Peptaibols are peptide antibiotics that typically feature an N‐terminal acetyl cap, a C‐terminal aminoalcohol, and high proportion of α‐aminoisobutyric acid (Aib) residues. To establish how each might affect the membrane‐activity peptaibols, biomimetic Aib foldamers with different lengths terminal groups were synthesised. Vesicle assays showed long (eleven residues) hydrophobic termini had highest ionophoric activity. acids or primary amides inhibited activity, while replacement...
A bis(cyclam)-capped cholesterol lipid designed to bind C-X-C chemokine receptor type 4 (CXCR4) was synthesised in good overall yield from 4-methoxyphenol through a seven step synthetic route, which also provided bis(cyclam) intermediate bearing an octaethyleneglycol-primary amine that can be easily derivatised. This water soluble and self-assembled into micellar non-micellar aggregates at concentrations above 8 μM. The bioactivity of the assessed using primary chronic lymphocytic leukaemia...