A5089085525- Bone fractures and treatments
- Education, Psychology, and Social Research
- Mesenchymal stem cell research
- Osteoarthritis Treatment and Mechanisms
- Orthopedic Surgery and Rehabilitation
- Periodontal Regeneration and Treatments
- Orthopaedic implants and arthroplasty
- Elbow and Forearm Trauma Treatment
- Shoulder Injury and Treatment
- Oral and Maxillofacial Pathology
- Intestinal Malrotation and Obstruction Disorders
- Shoulder and Clavicle Injuries
- Hip and Femur Fractures
- Bone Tissue Engineering Materials
- Spine and Intervertebral Disc Pathology
- Pleural and Pulmonary Diseases
- Medical and Biological Sciences
- Abdominal Surgery and Complications
- Bone Tumor Diagnosis and Treatments
- Sports injuries and prevention
- Bone health and treatments
- Gallbladder and Bile Duct Disorders
- Neuroendocrine Tumor Research Advances
- Vascular Malformations and Hemangiomas
- Pediatric Hepatobiliary Diseases and Treatments
University Hospital Brno
2010-2025
Masaryk University
2007-2025
Ministry of Health
2014
Universitätsklinik für Chirurgie Wien
2009-2010
Trauma Hospital of Brno
2009
Temporary hemiepiphyseodesis using figure-eight plates is currently one of the main surgical techniques to correct axial deformities lower limbs in paediatric patients. Comprehensive analysis, indication and monitoring treatment are basic prerequisites for successful therapy. The aim study was analyse parameters that could become an alternative standard used nowadays, namely inserted screw angle (SA), a new parameter - condylar ratio (CR). included 47 patients treated at Department...
The aim of this experimental study on New Zealand's white rabbits was to find differences in the results treating distal physeal femoral defect by transplantation autologous or allogeneic mesenchymal stem cells (MSCs). After excision a created bone bridge physis right femur, modified composite scaffold with MSCs transplanted into defect. In animal Group A (n = 11) autogenous were implanted; B 15) implanted. An iatrogenic left femur each not treated served as control. euthanized four months...
Mechanical properties of scaffolds seeded with mesenchymal stem cells used for cartilage repair seem to be one the critical factors in possible joint resurfacing. In this paper, effect adding hyaluronic acid, hydroxyapatite nanoparticles or chitosan nanofibers into cross-linked collagen I on mechanical response lyophilized porous scaffold has been investigated dry state at 37 oC under tensile loading. Statistical significance results was evaluated using ANOVA analysis. The showed that...
Benign bone lesions in children although rare, can result a pathological fracture. Although their etiology and pathogenesis are not yet entirely clear, the phenomenon of spontaneous healing is well known. Nevertheless, some benign unlikely to heal spontaneously due patient's age or high risk fracture deformity lesion's location size. The following study presents our results after treatment these cysts with chronOS Inject.From June 2004 May 2007 23 patients 24 were treated Inject, an...
The aim of this study was to evaluate macroscopically, histologically and immunohistochemically the quality newly formed tissue in iatrogenic defects articular cartilage femur condyle miniature pigs treated with clinically used method microfractures comparison transplantation a combination composite scaffold allogeneic mesenchymal stem cells (MSCs) or alone. cartilaginous filling after MSCs (Group A) had 60 % cases macroscopically smooth surface. In all lesions alone B) C), erosions/fissures...
This study appears from an experiment previously carried out in New Zealand white rabbits. Allogenic mesenchymal stem cells (MSCs) were transplanted into iatrogenically-created defect the lateral section of distal physis left femur 10 miniature pigs. The right with same served as a control. To transfer MSCs, freshly prepared porous scaffold was used, based on collagen and chitosan, constituting compact tube which MSCs implanted. pigs euthanized four months after transplantation. On average,...
Physeal cartilage is known to have poor self-repair capacity after injury. Evaluation of the ability cultured mesenchymal stem cells repair damaged physis topic current research. In 10 immature New Zealand white rabbits autogenous were transplanted into a iatrogenic physeal defect in lateral portion distal growth plate right femur. The same without transplantation left femoral served as control. our study, we used own technique implantation MSCs with newly modified gel scaffold (New...
This study describes the results achieved using a combination of allogeneic mesenchymal stem cells (MSCs) with chondrocytes (CHC) and new scaffold consisting type-I collagen chitosan nanofibers in prevention partial growth plate arrest after iatrogenic injury pigs.The miniature pig was selected as an experimental model to compare left femoral bones (MSCs CHC transplantation into distal defect) right (scaffold alone transplantation). The group consisted 10 animals. Bone marrow from os ilium...
PURPOSE OF THE STUDYFifth metatarsal fractures, in particular so-called Jones are relatively common injuries both the general population and athletes.Although discussions about whether surgical or conservative solution should be preferred ongoing for decades, there is no clear consensus.Here, we aimed to prospectively compare results of osteosynthesis using Herbert screw with patients from our department. MATERIAL AND METHODSPatients 18-50 years presenting department fracture meeting further...
Mesenchymal stem cells (MSCs) from bone marrow are multipotent capable of forming cartilage, bone, and other connective tissues. The objective this study was to determine whether the use allogenic mesenchymal could functionally heal a defect in distal femoral physis rabbits without immunosuppressive therapy. A iatrogenic created lateral condyle thirty-two New Zealand white rabbits, 7 weeks old, weighing 2.25 ± 0.24 kg. Each defect, 3.5 mm width 12 length, right treated with new composite...
Computed tomography (CT) is an effective diagnostic modality for three-dimensional imaging of bone structures, including the geometry their defects. The aim study was to create and optimize 3D geometrical real plastic models distal femoral component knee with joint surface Input data included CT images stifle joints in twenty miniature pigs iatrogenic osteochondrosis-like lesions medial condyle left knee. animals were examined eight sixteen weeks after surgery. Philips MX 8000 View...
This synoptic study gives a concise overview of current knowledge bone healing, the role mesenchymal stem cells in tissue regeneration and contemporary possibilities supporting damaged bone. Attention research concerning healing fractures with extensive loss following trauma, treatment belatedly or non-healing segmental defects tumour resection, is focused on development three-dimensional scaffolds planted that might be used for reconstruction such large lesions. Presented are...
The aim of the study was to verify whether there is a difference in lengthwise growth femurs and their angular deformity when comparing preventive vs. therapeutic transplantation allogeneic mesenchymal stem cells (MSCs) an iatrogenic defect distal physis femur. Modified composite chitosan/collagen type I scaffold with MSCs transplanted iatrogenically created cartilage lateral condyle left femur 20 miniature male pigs. In Group A animals (n = 10) were immediately after creating (preventive...
This review briefly summarizes existing results and main trends in experimental studies dealing with the possibilities of use biomaterials mesenchymal stem cells treatment diseases affecting tissues derived from mesoderm, i.e. articular cartilage, meniscus, physis, bone, ligaments tendons. The aim these is to find optimal forms individual parts musculoskeletal system using methods tissue engineering cell transplantation.
Owing to advances in operative techniques and biotechnology, bone replacement biocompatible materials have recently come into focus for orthopaedic trauma surgeons. Bone lytic lesions, such as tumorous defects, diseases simulating cancer, chronic inflammatory lesions or skeletal injuries, often require stabilisation of the skeleton treatment affected. Juvenile cysts are benign posing a threat compactness childhood. They benign, fluid containing cavities, lined with membrane consisting thin...
Many growth factors have been studied as additives accelerating lumbar fusion rates in different animal models. However, their low hydrolytic and thermal stability both vitro vivo limits workability use. In the proposed work, a stabilized vasculogenic prohealing fibroblast factor-2 (FGF2-STAB®) exhibiting functional half-life at 37 °C more than 20 days was applied for combination with bioresorbable scaffold on porcine An experimental study designed to investigate intervertebral efficiency...
At present, attention is focused on research into possibilities of healing large bone defects by the method mini-invasive osteosynthesis, using implantation biomaterials and mesenchymal stem cells (MSCs). This study evaluates segmental femoral in miniature pigs based radiological determination callus: cortex ratio at 16 weeks after ostectomy. The size formed callus was significantly larger ( p < 0.05) animals transplantation an autogenous cancellous graft (group A, : 1.77 ± 0.33) compared...
PURPOSE OF THE STUDYThe aim of the study was to evaluate results elastic stable intramedullary nailing (ESIN).used in treatment children with femoral shaft fractures, a focus on post-traumatic rotational deformity assessed by magnetic resonance imaging (MRI).An alternative hypothesis established assuming significantly high incidence this complication.Also, advantages MRI for diagnosis were comparison ultrasonography and physical examination.
A short liposome exposure to ultrasonic waves with the intensity of I = 2 W/cm at frequency f 1 MHz was found be a sufficient tool for opening. In addition, shock-wave proved also content release. Both methods are useful tools used as control mechanisms drug delivery systems based on liposomes. However, due better focusing and low non-thermal side effects, shock-waves probably have markedly higher potential successful use.
PURPOSE OF THE STUDYThe presented experimental study describes the results of using a combination allogeneic mesenchymal cells (MSCs) with chondrocytes (CHCs) and novel scaffold based on type I collagen chitosan fibres.This biocomposite was transplanted into defect produced by excision bone bridge to induce new cartilaginous tissue formation.The left femur treated transplantation distal epiphysis; right implantation only served as control.A better therapeutic result therefore expected in...