A5101444856- Click Chemistry and Applications
- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Advanced biosensing and bioanalysis techniques
- Chemical Synthesis and Analysis
- Microbial Metabolites in Food Biotechnology
- Molecular Sensors and Ion Detection
- Analytical Chemistry and Sensors
- Monoclonal and Polyclonal Antibodies Research
- Phytoestrogen effects and research
- Studies on Chitinases and Chitosanases
- Luminescence and Fluorescent Materials
- Electrochemical sensors and biosensors
- Enzyme Catalysis and Immobilization
- Phytochemical compounds biological activities
- Fluorine in Organic Chemistry
- Antenna Design and Analysis
- Photonic and Optical Devices
- Seaweed-derived Bioactive Compounds
- Amino Acid Enzymes and Metabolism
- Fungal Biology and Applications
- Microwave Engineering and Waveguides
- Liver physiology and pathology
- History and advancements in chemistry
- Polysaccharides and Plant Cell Walls
Chinese Academy of Medical Sciences & Peking Union Medical College
2020-2024
Yunnan University
2023
Feed Research Institute
2006-2019
Chinese Academy of Agricultural Sciences
2006-2019
Huazhong Agricultural University
2012
Fudan University
2007
A series of naphthalimide-tetrazines were developed as bioorthogonal fluorogenic probes, which could produce significant fluorescence enhancement, notable aggregation-induced emission (AIE) characters and multicolor emissions after reaction with strained dienophiles. Manipulating the π-bridge in fluorophore skeleton allows fine-tuning wavelength influences AIE-active properties. With these we succeeded no-wash protein labeling mitochondria-selective imaging live cells.
An endo-1,4- β -xylanase-encoding gene, xyn11B , was cloned from the thermophilic fungus Humicola insolens Y1. The gene encodes a multimodular xylanase that consists of typical hydrophobic signal sequence, catalytic domain glycoside hydrolase (GH) family 11, glycine-rich linker, and 1 carbohydrate binding module (CBM1). Deduced Xyn11B shares highest identity 74% with putative Podospora anserina S mat+. Recombinant successfully expressed in Pichia pastoris purified to electrophoretic...
Glycoside hydrolase (GH) family 12 comprises enzymes with a wide range of activities critical for the degradation lignocellulose. However, important roles loop regions GH12 in substrate specificity and catalytic efficiency remain poorly understood. This study examined how 3 region affects enzymatic properties glucanases using NfEG12A from Neosartorya fischeri P1 EG (PDB 1KS4) Aspergillus niger Acidophilic thermophilic had highest (kcat/Km , 3,001 263 ml/mg/s toward lichenin carboxymethyl...
A novel β-glucosidase gene (Bgl3B) of glycoside hydrolase (GH) family 3 was cloned from the thermophilic fungus Talaromyce leycettanus JM12802 and successfully expressed in Pichia pastoris. The deduced Bgl3B contains 860 amino acid residues with a calculated molecular mass 91.2 kDa. purified recombinant exhibited maximum activities at pH 4.5 65°C remained stable temperatures up to 60°C 3.0-9.0, respectively. enzyme broad substrate specificities, showing β-glucosidase, glucanase, cellobiase,...
Wheat bran is an effective raw material for preparation xylooligosaccharides; however, current research mainly focuses on alkali extraction and enzymatic hydrolysis methods. Since ester bonds are destroyed during the process, xylanase arabinofuranosidase used to hydrolyze xylooligosaccharides. However, costs very high, method also causes pollution. Therefore, this study elucidating a efficiently directly degrade destarched wheat bran. First, acidic acetyl xylan esterase (AXE) containing...
Comprehensive Summary A nitrone‐modified 1,8‐naphthalimide was designed as a novel bioorthogonal‐activated turn‐on probe based on strain‐promoted alkyne‐nitrone cycloaddition (SPANC). The bioorthogonal cycloadducts were subsequently transformed into fluorescent rearrangement products by photo‐acceleration, which exhibited significant fluorescence enhancement, large stokes shift, and high quantum yield. DFT calculations performed to elucidate the OFF‐ON mechanism. This fluorogenic strategy...
We designed four 4‐hydrazone‐1,8‐naphthalimides as novel fluorogenic click probes, from which the in situ generated nitrile imines react with bicyclo[6.1.0]nonyne through strain‐promoted 1,3‐dipolar cycloaddition. The resulting pyrazole cycloadducts were found to be highly fluorescent compelling photophysical properties, including large stokes shift (up 152 nm), high fluorescence quantum yields 0.85), and notable enhancement 196.9‐fold). Density functional theory calculations carried out...
We reported a series of nitroso-modified naphthylene-based fluorophores as novel bioorthogonal fluorescence turn-on probes. The cycloadducts from nitroso-diene Diels–Alder reaction could be either photochemically or spontaneously transformed into highly fluorescent rearrangement products with remarkable photophysical properties including significant enhancement, large Stokes shift, high quantum yield, superior photostability, and distinct solvatochromic effect. This strategy is suitable for...
A smart fluorescence "turn-on" probe which contained a dansyl amide fluorophore and an N-oxide group was designed based on the bioorthogonal decaging reaction between boron reagent. The proceeds in rapid kinetics (k
OF THE DISSERTATION Design and Analysis of Amino Acid Supplementation in Hepatocyte Culture Using vitro Experiment Mathematical Modeling By HONG YANG Dissertation Directors: Dr. Marianthi G. Ierapetritou, Ph.D. Charles M. Roth, Extracorporeal bioartificial liver (BAL) devices, involving primary hepatocytes, represent a promising option to provide temporary support for patients with failure. Current use BAL is challenged by development techniques long-term culture hepatocytes during plasma...
The Cover Feature shows the fluorescent “turn-off” and “turn-on” states of 1,8-naphthalimide fluorogenic click probes though hand-drawn cartoon lotus (credit: Xiang Li, one authors). “In bud” state represents 4-hydrazone-1,8-naphthalimides, which is in state, while “blooming” cycloadducts after reaction. “open” “close” petals reflect well “fluorescent” “nonfluorescent” properties these probes. More information can be found Communication by Y. Tian et al.