A5091317996- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Phytase and its Applications
- Enzyme Catalysis and Immobilization
- Studies on Chitinases and Chitosanases
- Protein Hydrolysis and Bioactive Peptides
- Microbial Metabolites in Food Biotechnology
- Polysaccharides and Plant Cell Walls
- Iron Metabolism and Disorders
- Probiotics and Fermented Foods
- Gut microbiota and health
- Animal Nutrition and Physiology
- Folate and B Vitamins Research
- Trace Elements in Health
- Diet and metabolism studies
- Metabolomics and Mass Spectrometry Studies
- Aquaculture Nutrition and Growth
- Natural product bioactivities and synthesis
- Liver Disease Diagnosis and Treatment
- Carbohydrate Chemistry and Synthesis
- Antimicrobial Peptides and Activities
- Peptidase Inhibition and Analysis
- Transgenic Plants and Applications
- Antioxidant Activity and Oxidative Stress
- Ruminant Nutrition and Digestive Physiology
Feed Research Institute
2016-2025
Ministry of Agriculture and Rural Affairs
2019-2025
Chinese Academy of Agricultural Sciences
2016-2025
Ningbo University
2024
Tsinghua University
2023
Guangxi Buffalo Research Institute
2020
State Council of the People's Republic of China
2019
Texas A&M University
2018
The University of Texas MD Anderson Cancer Center
2018
The University of Texas Health Science Center at Houston
2018
Phytate is the most abundant organic phosphorus compound in nature, and microbial mineralization of phytate by phytase a key process for recycling biosphere. In present study, beta-propeller (BPP) gene fragments were readily amplified from intestinal contents grass carp (Ctenopharyngodon idellus) directly or phytate-degrading isolates same source, confirming widespread occurrence BPP aquatic communities. The amounts sequences collected using these two methods differed (88 distinct genes...
Abstract Background Fatty liver hemorrhage syndrome (FLHS) becomes one of the most major factors resulting in laying hen death for caged egg production. This study aimed to investigate therapeutic effects Lactiplantibacillus plantarum ( Lp. ) FRT4 on FLHS model with a focus lipid metabolism, and gut microbiota. Results The hens was established by feeding high-energy low-protein (HELP) diet, treatment groups were fed HELP diet supplemented differential proportions FRT4. results indicated that...
Abstract Background The incidence of obesity is increasing worldwide, and it a risk factor for diabetes, dyslipidemia, nonalcoholic fatty liver disease. Our previous study had demonstrated that high-fat diet induced increased weight gain, fat weight, serum cholesterol, triglyceride, ATL levels in liver, influenced the diversity composition cecal microbiota mice. Hence, this aimed to investigate roles gut microbially derived metabolites between obese lean mice, focusing on their association...
Fatty liver hemorrhage syndrome (FLHS) has become one of the major factors leading to death laying hen in caged egg production. FLHS is commonly associated with lipid peroxidation, hepatocyte injury, decreased antioxidant capacity, and inflammation. However, there are limited evidences regarding preventive effect Lactiplantibacillus plantarum on hens its mechanisms. Our previous results showed that Lp. FRT4 alleviated by regulating metabolism, but did not focus anti-inflammatory functions...
Xylanases are utilized in a variety of industries for the breakdown plant materials. Most native and engineered bifunctional/multifunctional xylanases have separate catalytic domains within same polypeptide chain. Here we report new bifunctional xylanase (XynBE18) produced by Paenibacillus sp. E18 with beta-1,3-1,4-glucanase activities derived from active center substrate competition assays site-directed mutagenesis Glu residues (E129A E236A). The gene consists 981 bp, encodes 327 amino...
A β-mannanase gene, man5AP13, was cloned from Phialophora sp. P13 and expressed in Pichia pastoris. The deduced amino acid sequence of the mature enzyme, MAN5AP13, had highest identity (53%) with glycoside hydrolase family 5 Bispora MEY-1. purified recombinant acidophilic stable, exhibiting maximal activity at pH 1.5 retaining >60% initial over range 1.5−7.0. optimum temperature 60 °C. specific activity, Km Vmax for locust bean gum substrate were 851 U/mg, 2.5 mg/mL, 1667.7 U/min·mg,...
Improving the hydrolytic performance of xylanolytic enzymes on arabinoxylan is importance in ethanol fermentation industry. Supplementation debranching (arabinofuranosidase) and depolymerizing (xylanase) a way to address problem. In present study, we identified bifunctional α-l-arabinofuranosidase/endo-xylanase (Ac-Abf51A) glycoside hydrolase family 51 Alicyclobacillus sp. strain A4. Its biochemical stability great hydrolysis efficiency against complex biomass make it potential candidate for...
ABSTRACT N -Glycosylation can modulate enzyme structure and function. In this study, we identified two pepsin-resistant histidine acid phosphatase (HAP) phytases from Yersinia kristensenii (YkAPPA) rohdei (YrAPPA), each having an -glycosylation motif, one pepsin-sensitive HAP phytase enterocolitica (YeAPPA) that lacked site. Site-directed mutagenesis was employed to construct mutants by altering the status of enzyme, mutant wild-type enzymes were expressed in Pichia pastoris for biochemical...