A5008818859- Photosynthetic Processes and Mechanisms
- Algal biology and biofuel production
- Microbial Metabolic Engineering and Bioproduction
- Plant Disease Resistance and Genetics
- Biocrusts and Microbial Ecology
- Mitochondrial Function and Pathology
- ATP Synthase and ATPases Research
- Plant-Microbe Interactions and Immunity
- Protein Structure and Dynamics
- Plant Pathogens and Fungal Diseases
- Bacterial Genetics and Biotechnology
- Enzyme Structure and Function
- Insect symbiosis and bacterial influences
- Fungal and yeast genetics research
- Wheat and Barley Genetics and Pathology
- Hemoglobin structure and function
- Bacteriophages and microbial interactions
- CRISPR and Genetic Engineering
- Photoreceptor and optogenetics research
- Composting and Vermicomposting Techniques
- Plant Virus Research Studies
- Biofuel production and bioconversion
- Light effects on plants
- Waste Management and Recycling
- Protist diversity and phylogeny
Michigan State University
2016-2024
University of Michigan
2018-2021
Pennsylvania State University
2013-2014
Carboxysomes are protein-based bacterial organelles encapsulating key enzymes of the Calvin-Benson-Bassham cycle. Previous work has implicated a ParA-like protein (hereafter McdA) as important for spatially organizing carboxysomes along longitudinal axis model cyanobacterium Synechococcus elongatus PCC 7942. Yet, how self-organization McdA emerges and contributes to carboxysome positioning is unknown. Here, we identify small protein, termed McdB that localizes drives emergent oscillatory...
Carboxysomes are protein-based organelles that essential for allowing cyanobacteria to fix CO2. Previously, we identified a two-component system, McdAB, responsible equidistantly positioning carboxysomes in the model cyanobacterium Synechococcus elongatus PCC 7942 (MacCready JS, Hakim P, Young EJ, Hu L, Liu J, Osteryoung KW, Vecchiarelli AG, Ducat DC. 2018. Protein gradients on nucleoid position carbon-fixing of cyanobacteria. eLife 7:pii:e39723). McdA, ParA-type ATPase, nonspecifically...
Abstract Carboxysomes are protein‐based organelles essential for carbon fixation in cyanobacteria and proteobacteria. Previously, we showed that the cyanobacterial nucleoid is used to equally space out β‐carboxysomes across cell lengths by a two‐component system (McdAB) model cyanobacterium Synechococcus elongatus PCC 7942. More recently, found McdAB systems widespread among β‐cyanobacteria, which possess β‐carboxysomes, but absent α‐cyanobacteria, structurally phyletically distinct...
Summary The oscillatory Min system of Escherichia coli defines the cell division plane by regulating site FtsZ‐ring formation and represents one best‐understood examples emergent protein self‐organization in nature. patterns Min‐system proteins MinC, MinD MinE (MinCDE) are strongly dependent on geometry membranes they bind. Complex internal within cyanobacteria could disrupt this sterically occluding or sequestering MinCDE from plasma membrane. Here, it was shown that cyanobacterium...
Cyanobacteria are emerging as alternative crop species for the production of fuels, chemicals, and biomass. Yet, success these microbes depends on development cost-effective technologies that permit scaled cultivation cell harvesting. Here, we investigate feasibility engineering morphology to improve biomass recovery decrease energetic costs associated with lysing cyanobacterial cells. Specifically, modify levels Min system proteins in Synechococcus elongatus PCC 7942. The has established...
Tar spot is a foliar disease of corn threatening production across the Americas. The was first documented in Mexico 1904 and now present 15 additional countries throughout Central America, South Caribbean. Researchers growers Caribbean consider tar to be complex caused by multiple fungal pathogens. When environmental conditions are conducive for infection, these regions have experienced yield losses that can reach up 100%. In 2015, detected United States time Illinois Indiana. Since has...
Tar spot is a devasting corn disease caused by the obligate fungal pathogen Phyllachora maydis. Since its initial identification in United States 2015, P. maydis has become an increasing threat to production. Despite this, remained largely understudied at molecular level, due difficulties surrounding lifestyle. Here, we generated significantly improved nuclear and mitochondrial genome, using combination of long- short-read technologies, also provide first transcriptomic analysis primary tar...
The cytoskeletal Filamenting temperature-sensitive Z (FtsZ) ring is critical for cell division in bacteria and chloroplast photosynthetic eukaryotes. While bacterial FtsZ rings are composed of a single FtsZ, except the basal glaucophytes, involves two heteropolymer-forming isoforms: FtsZ1 FtsZ2 green lineage FtsZA FtsZB red algae. probably arose by duplication more ancestral FtsZA, respectively. We expressed fluorescent fusions from diverse organisms heterologous system to compare their...
is an ascomycete foliar fungal pathogen and the causal agent of tar spot in maize. Although
ABSTRACT Many companies, restaurants, colleges, as well individual homeowners, have initiated green marketing campaigns, and the composting of food yard wastes has become commonplace. Thus, it is essential to obtain a more complete understanding microbial populations present in such processes. The focus this study was identify bacterial static windrow compost pile on college campus containing trim tree debris. Given that during winter uniquely characterized by pre-mesophilic stage addition...
ABSTRACT Cyanobacteria are the prokaryotic group of phytoplankton responsible for a significant fraction global CO 2 fixation. Like plants, cyanobacteria use enzyme Ribulose 1,5-bisphosphate Carboxylase/Oxidase (RuBisCO) to fix into organic carbon molecules via Calvin-Benson-Bassham cycle. Unlike evolved concentrating organelle called carboxysome - proteinaceous compartment that encapsulates and concentrates RuBisCO along with its substrate. In rod-shaped cyanobacterium Synechococcus...
Photosynthetic cyanobacteria are responsible for almost half of global CO 2 fixation. Due to eutrophication, rising temperatures, and increasing atmospheric concentrations, have gained notoriety their ability form massive blooms in both freshwater marine ecosystems across the globe.
Abstract Bacterial microcompartments (BMC) are protein-based organelles broadly distributed across all bacterial phyla and subclassified into ≥60 functional variants. Despite their evolutionary metabolic diversity, shell proteins that structurally compose the BMC surface closely related classes. Herein, we sought to identify molecular physiological features could promote independent operation of more than one type within same cell by reducing inter-organelle cross-talk proteins. We...
ABSTRACT Phyllachora maydis is an ascomycete foliar fungal pathogen and the causal agent of tar spot in maize. Though P. considered one most economically important pathogens maize, our general knowledge trophic lifestyle functional role effector proteins from this remains limited. Here, we utilized a genome-informed approach to predict functionally characterized subset candidate effectors pathogen. Leveraging recent genome annotation CATAStrophy pipeline, show encodes predicted...
Carboxysomes are protein-based bacterial organelles that encapsulate a key enzyme of the Calvin-Benson-Bassham cycle. Previous work has implicated ParA-like protein (hereafter McdA) as important for spatially organizing carboxysomes along longitudinal axis model cyanobacterium Synechococcus elongatus PCC 7942. Yet, how self-organization McdA emerges and contributes to carboxysome positioning is unknown. Here, we show small protein, termed McdB, localizes through interactions with shell...
In Brief We describe a novel positioning system that utilizes the cyanobacterial nucleoid to segregate, transport and equidistantly position carbon-fixation machinery (carboxysomes). Summary Carboxysomes are protein-based bacterial organelles encapsulate key enzyme of Calvin-Benson-Bassham cycle. Previous work has implicated ParA-like protein (hereafter McdA) as important for spatially organizing carboxysomes along longitudinal axis model cyanobacterium Synechococcus elongatus PCC 7942. Yet,...
Summary Carboxysomes are protein-based organelles that essential for allowing cyanobacteria to fix CO 2 . Previously we identified a two-component system, McdAB, responsible equidistantly positioning carboxysomes in the model cyanobacterium Synechococcus elongatus PCC 7942. McdA, ParA-type ATPase, non-specifically binds nucleoid presence of ATP. McdB, novel factor directly carboxysomes, displaces McdA from nucleoid. Removal vicinity by McdB causes global break symmetry, and carboxysome...
Summary Carboxysomes are protein-based organelles essential for carbon fixation in cyanobacteria and proteobacteria. Previously, we showed that the cyanobacterial nucleoid is utilized as a surface equidistant-spacing of β-carboxysomes across cell lengths by two-component system (McdAB) model cyanobacterium Synechococcus elongatus PCC 7942. More recently, found McdAB systems widespread among β-cyanobacteria, which possess β-carboxysomes, but absent α-cyanobacteria, structurally distinct...