A5074605598- Image Processing Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- Digital Holography and Microscopy
- Quinazolinone synthesis and applications
- Click Chemistry and Applications
- Biosensors and Analytical Detection
- ATP Synthase and ATPases Research
- Cell Image Analysis Techniques
- Advanced Biosensing Techniques and Applications
- Mitochondrial Function and Pathology
- Nanoparticle-Based Drug Delivery
- Cancer therapeutics and mechanisms
- Advanced Image Processing Techniques
- Microfluidic and Capillary Electrophoresis Applications
- Nanoplatforms for cancer theranostics
- AI in cancer detection
- Synthesis and Characterization of Heterocyclic Compounds
- Cervical Cancer and HPV Research
- Metal complexes synthesis and properties
- Autophagy in Disease and Therapy
- Advanced Optical Imaging Technologies
- Luminescence Properties of Advanced Materials
- Cancer Genomics and Diagnostics
- Single-cell and spatial transcriptomics
- Allergic Rhinitis and Sensitization
Center for Systems Biology
2015-2019
Massachusetts General Hospital
2015-2019
Harvard University
2016-2019
University of Southern California
2009-2016
Extracellular vesicles, including exosomes, are nanoscale membrane particles that carry molecular information on parental cells. They being pursued as biomarkers of cancers difficult to detect or serially follow. Here we present a compact sensor technology for rapid, on-site exosome screening. The is based an integrated magneto–electrochemical assay: exosomes immunomagnetically captured from patient samples and profiled through electrochemical reaction. By combining magnetic enrichment...
Adverse food reactions, including allergies, sensitivities, and autoimmune reaction (e.g., celiac disease) affect 5–15% of the population remain a considerable public health problem requiring stringent avoidance epinephrine availability for emergency events. Avoiding problematic foods is practically difficult, given current reliance on prepared out-of-home meals. In response, we developed portable, point-of-use detection technology, termed integrated exogenous antigen testing (iEAT). The...
Background Recently, there has been a surge of interest in developing compounds selectively targeting mitochondria for the treatment neoplasms. The critical role cellular metabolism and respiration supports this therapeutic rationale. Dysfunction processes energy production contributes to attenuation response pro-apoptotic stimuli increased ROS both which are implicated initiation progression most human cancers. Methodology/Principal Findings A high-throughput MTT-based screen over 10,000...
Significance Smartphones and wearable electronics have advanced tremendously over the last several years but fall short of allowing their use for molecular diagnostics. We herein report a generic approach to enable diagnostics on smartphones. The method utilizes molecular-specific microbeads generate unique diffraction patterns “blurry beads” which can be recorded deconvoluted by digital processing. applied system resolve individual precancerous cancerous cells as well detect...
Abstract Mitochondria, which are essential organelles in resting and replicating cells, can vary number, mass shape. Past research has primarily focused on short-term molecular mechanisms underlying fission/fusion. Less is known about longer-term mitochondrial behavior such as the overall makeup of cell populations’ morphological patterns whether these be used biomarkers drug response human cells. We developed an image-based analytical technique to phenotype morphology different cancers,...
Serial tissue sampling has become essential in guiding modern targeted and personalized cancer treatments. An alternative to image guided core biopsies are fine needle aspirates (FNA) that yield cells rather than tissues but much better tolerated have lower complication rates. The efficient pathway analysis of such the clinic been difficult, time consuming costly. Here we develop an antibody-DNA barcoding approach where harvested can be rapidly re-stained through use custom designed...
Pancreatic ductal adenocarcinoma (PDAC) is an aggressive disease with poor prognosis and limited therapeutic options. Therefore, there urgent need to identify new, safe, targeted therapeutics for effective treatment of late as well early stage disease. Plectin-1 (Plec-1) was recently identified specific biomarker detecting PDAC at stage. We envisioned that multivalent attachment nanocarriers incorporating certain drugs Plec-1-derived peptide would increase binding affinity impart high...
Abstract Lens-free digital in-line holography (LDIH) is a promising technology for portable, wide field-of-view imaging. Its resolution, however, limited by the inherent pixel size of an imaging device. Here we present new computational approach to achieve sub-pixel resolution LDIH. The developed method sparsity-based reconstruction with capability handle non-linear nature We systematically characterized algorithm through simulation and LDIH studies. achieved spatial down one-third size,...
The global burden of cancer, severe diagnostic bottlenecks in underserved regions, and underfunded health care systems are fueling the need for inexpensive, rapid, treatment-informative diagnostics. On basis advances computational optics deep learning, we have developed a low-cost digital system, termed AIDA (artificial intelligence diffraction analysis), breast cancer diagnosis fine needle aspirates. Here, show high accuracy (>90%) (i) recognizing cells directly from patterns (ii)...
We report a sensitive and versatile biosensing approach, LUCID (luminescence compact in vitro diagnostics), for quantitative molecular cellular analyses. uses upconversion nanoparticles (UCNPs) as luminescent reporters mutually exclusive photoexcitation read-out sequences implemented on smartphone. The strategy improves imaging signal-to-noise ratios, eliminating interference from excitation sources minimizing autofluorescence, thus enables filterless imaging. Here we developed miniaturized...
Most deaths (80%) from cervical cancer occur in regions lacking adequate screening infrastructures or ready access to them.In contrast, most developed countries now embrace human papillomavirus (HPV) analyses as standalone screening; this transition threatens further widen the resource gap.Methods: We describe development of a DNA-focused digital microholography platform for point-of-care HPV screening, with automated readouts driven by customized deep-learning algorithms.In presence...
Low-cost, rapid and accurate detection technologies are key requisites to cope with the growing global cancer challenges. The need is particularly pronounced in resource-limited settings where treatment opportunities often missed due absence of timely diagnoses. We herein describe a Holographic Assessment Lymphoma Tissue (HALT) system that adopts smartphone as basis for molecular diagnostics. detects malignant lymphoma cells labeled marker-specific microbeads produce unique holographic...
A smartphone-based assay is developed to detect soluble protein markers in point-of-care settings.
Background and Purpose Pancreatic cancer is characterized by alterations in several key signalling proteins, including increased expression activity of the Src tyrosine kinase focal adhesion ( FAK ), which have been linked to its chemoresistance. Sustained inhibition reactivates survival pathways regulated transcription factor STAT3 , also leading resistance. Therefore, simultaneously targeting / could provide an important strategy for treating pancreatic cancer. Recently, we described novel...
Abstract Mitochondria, critical organelles in quiescent and dividing cells, have been shown to vary number, mass, shape. While previous work has focused on understanding molecular mechanisms underlying changes normal immortalized less is known about longer-term mitochondrial behavior such as morphological patterns individual cells overall populations, the heterogeneity functional consequences of these patterns, ultimately how potential can be exploited clinic. Indeed, while studies conducted...
Abstract Proliferation under selective pressures exerted by an unstable microenvironment, requires tumor cells employ adaptive mechanisms that confer growth advantage. Adaptation to adverse conditions results in cellular phenotypes typify neoplastic transformation and offer unique opportunities for targeting of cancer cells. Agents target cell mitochondria with high selectivity hold clinical significance due the adaptive, modulatory essential role this organelle energy production, metabolism...
Abstract Pancreatic cancer is a complex disease. Late stage detection, poor prognosis, and resistance to available chemotherapeutics highlight the need discover new potent anticancer agents for its effective treatment. Altered cellular bioenergetics oxidative stress are emerging hallmarks of most forms including pancreatic cancer. Cancer cells more prone reactive oxygen species (ROS)-mediated cell death due their inherent elevated basal as compared normal cells. Increased ROS levels could...
Abstract Oxidative stress is an important hallmark of cancer. Elevated levels intrinsic reactive oxygen species (ROS) make tumor cells more susceptible to exogenously induced oxidative stress. Excessive insults overwhelm the adaptive antioxidant capacity cancer and trigger ROS-mediated cell death. Increasing ROS production or decreasing scavengers shows potential for selectively targeting that are under persistent Recently, we have discovered novel class quinazolinediones exert their...