Abstract Objective To assess the possible association between PTPN22 gene 1858C→T polymorphism and predisposition clinical expression of 2 systemic autoimmune diseases, rheumatoid arthritis (RA) lupus erythematosus (SLE). Methods Our study population consisted 826 RA patients, 338 SLE 1,036 healthy subjects. All subjects were Spanish Caucasian origin. Genotyping was performed by real‐time polymerase chain reaction technology, using TaqMan 5′‐allele discrimination assay. Results The overall...

Genetic host factors may modify the course of hepatitis C virus (HCV) infection. Very recently, a genome-wide scan that reported association IL28B locus with response to treatment in HCV infection was published. The aim current study investigate relationship this outcome cohort constituted by total 731 Spanish individuals. From these, 284 were subjects persistent infection, 69 individuals who naturally cleared virus, and 378 noninfected subjects. Genotyping rs12979860 (C>T) performed...

OBJECTIVES--A self referred group of four workers from a factory producing scouring powder with high silica content showed surprisingly number features compatible connective tissue disease. Further subjects working at the same were subsequently studied to evaluate relation between this exposure and development autoimmune processes. METHODS--A total 50 (44 women, six men; mean (SD) age 43.7 (5.5) years; duration employment 6.1 years) underwent prospective study including clinical history...

Monoclonal antibody IVD12 was used to isolate and characterize a human Ia molecule present on B cells that generally display DR4 or DR5 phenotypes. The specificity of binding peripheral blood from 75 normal individuals 19 homozygous lymphoblastoid cell lines identical the supertypic MB3 previously defined. Furthermore, IVD12-reactivity shown segregate with HLA in three informative families. In each family, positive for were also DR5. Using IVD12, has been isolated line PRIESS (DR4/4) by...

Clinical relevance of donor-specific antibodies (DSAs) detected by a single antigen Luminex virtual crossmatch in pre-transplant serum samples from patients with negative cytotoxicity-dependent complement is controversial. The aim this study was to analyse the influence positive outcome kidney transplantation.A total 892 who received graft deceased donors after cytotoxicity were included. Presence anti-human leucocyte (HLA) investigated using screening assay and anti-HLA specificities...

Summary Four patients of 283 liver-transplant recipients (1·4%) developed de novo immune-mediated hepatitis approximately 2 years after transplantation. Antibodies showing an unusual liver/kidney cytoplasmic staining pattern were detected in the sera all four and one them was used to screen a human liver cDNA expression library with aim identifying antigenic target these newly antibodies. After cloning sequencing gene, it identified as gene encoding glutathion-S-transferase T1 (GSTT1), 29-kD...

A new form of autoimmune hepatitis referred to as de novo, has been reported after liver transplantation during the past 5 years. The features are identical those classical (AIH), but facts involved in onset and outcome this type graft dysfunction still unclear. identification antibodies directed glutathione S-transferase T1 (GSTT1) sera patients with novo immune led us description an alloimmune reaction due a GSTT1 genetic incompatibility between donor recipient. We analyzed cohort 110...

Abstract Objective To investigate the possible association of CT60A/G marker with systemic lupus erythematosus (SLE) in Spanish patients, and to identify CTLA4 haplotype responsible for association, taking into account other polymorphisms described at positions −1722T/C, −319C/T, +49A/G, microsatellite (AT) n 3′‐untranslated region (3′‐UTR) gene. Methods Genotyping CT60 was performed 395 patients SLE 293 healthy controls using polymerase chain reaction (PCR)–restriction fragment length...

Abstract Objective. To analyze the associations of HLA class II antigens with rheumatoid arthritis (RA) in a Spanish population. Methods. We used DNA oligotyping to determine DR types, DQA1 and DQB1 alleles, DR4 variants 70 unrelated seropositive RA patients 189 healthy controls living Spain. Results. A significantly higher frequency was seen compared (relative risk [RR] = 2.40). The DR10 specificity correlated most strongly disease susceptibility (RR 3.84). significant decrease DR7 observed...

Cytotoxic T‐lymphocyte antigen 4 (CTLA4) polymorphisms located in the promotor region at positions −318 (C/T) and exon 1 (49 A/G) were investigated 138 Spanish patients (37 men 101 women) with rheumatoid arthritis 305 ethnically‐matched healthy controls. When allelic genotypic frequencies corresponding to CTLA4 position compared, no significant differences between controls found. However, when 49 A/G polymorphism was analysed, a increase of heterozygous individuals among female (48.5% vs....

Production of antibodies against donor-specific antigens is one the central mechanisms allograft rejection. This antibody-mediated rejection (AMR) evidenced by presence circulating and deposition complement component C4d on renal endothelium. Although anti-human leukocyte antigen (HLA) account for a high proportion AMR, in many cases anti-HLA cannot be demonstrated. In liver transplant, glutathione-S-transferase T1 (GSTT1) expressed graft may induce an antibody response leading to severe...

According to genome wide association (GWA) studies as well candidate gene approaches, Behçet's disease (BD) is associated with human leukocyte antigen (HLA)-A and HLA-B regions. The HLA-B51 has been consistently the disease, but role of other HLA class I molecules remains controversial. Recently, variants in non-HLA genes have also BD. aims this study were further investigate influence region BD explore relationship recently described be populations. This included 304 patients 313 ethnically...

The possible role of the functional polymorphism located in regulatory region monocyte chemoattractant protein-1 (MCP-1) gene susceptibility to systemic lupus erythematosus (SLE) was investigated. Two hundred and seventy-six SLE patients (among them, 99 with nephritis 55 cutaneous vasculitis) 194 ethnically matched healthy controls were included study. Genotyping for -2518 (A/G) MCP-1 performed by polymerase chain reaction-restriction fragment length (PCR-RFLP) analysis. No association...

Abstract: Twenty‐six unrelated Spanish Caucasian individuals affected by pemphigus vulgaris (PV) were HLA typed and frequencies compared with those of 200 ethnically matched healthy controls. Twenty‐three out 26 patients HLA‐DR4. The frequency HLA‐DR14 was also increased (31%; controls: 4%). Of the 23 positive for HLA‐DR4, 21 carried DRB1*0402 allele. Therefore, HLA‐DRB1*0402 among 81% (4% in controls; P =4.7times 10 ‐27 , OR=100.8). Interestingly, HLA‐DR13, a frequent HLA‐DR specificity...

Background The role of anti–human leukocyte antigen (HLA) donor-specific antibodies (DSA) detected by Luminex in the development rejection is not fully understood. Methods A study including 369 patients who received transplants from deceased donors with a negative complement-dependent cytotoxicity crossmatch (XM) was performed. From total patients, 151 underwent renal biopsy because dysfunction, whereas 218 remaining showed stable function, and no assumed. Diagnosis type based data. Results...

De novo immune hepatitis (DNIH) is a form of late graft dysfunction after liver transplantation. The fine mechanisms leading to the development DNIH are not known, and whether this rejection or result an auto/alloimmune injury has been established. In our patients, was always preceded by production donor-specific antibodies against glutathione S-transferase T1 (GSTT1) enzyme because genetic mismatch in which donors carried wild-type gene recipients displayed null genotype. Complement...

AIRE is a transcriptional regulator playing functional role in thymocyte education and negative selection by controlling the expression of peripheral antigens thymus. Recently, gene was identified as genetic risk factor for rheumatoid arthritis (RA) genome wide association (GWA) studies performed Japanese population. According to available data this restricted Asian However, different facts could influence lack Caucasian populations. The aim study further investigate possible susceptibility...

The presence of certain vitamin D receptor (VDR) genotypes has been associated with low bone mineral density (BMD) in elderly populations as well accelerated loss patients rheumatoid arthritis (RA). In the present study, VDR from 120 Spanish RA were investigated. Three gene polymorphisms (BsmI, ApaI and TaqI) investigated using polymerase chain reaction followed by enzymatic digestion. distributions allelic frequencies similar controls therefore no influence on susceptibility could be...

Summary The heavy ( H ) and light L chain V‐region sequences of eight human autoreactive immunoglobulin M (IgM) monoclonal antibodies (mAbs: BY‐4, BY‐7, BY‐12, IRM‐3, IRM‐7, IRM‐8, IRM‐10 CDC‐1) were determined at the cDNA level. All V families identified. Four different represented, 3 being most common as five mAbs (BY‐7, IRM‐8 used genetic elements this family, whereas 1, 2 4 only present in BY‐4 IRM‐10, respectively. IRM‐7 reacted with a variety self well non‐self antigens, thus...

We have analyzed DNA sequence polymorphisms of DQ alpha and beta chains from three haplotypes the DRw52 family: DR5 DQw1 (FPA, GM3106), DRw6 (CB6B, 10w9060), DQw3 (AMALA, 10w9064). The results indicate that arisen by recombination between DR 1 loci. This contrasts with our previous analysis DR4 DQ"Wa", DR3 DR7 haplotypes, all which appear to virtue beta. Thus, there be at least two different sites where has occurred within subregions. These differing patterns were interpreted in context...

The nucleotide and inferred amino acid sequence of a DRw10 beta chain was obtained from cDNA clones isolated DR1, heterozygous cell line. this gene distinctive, differing those all other defined DR types. shown by transfection experiments to encode polymorphic epitope recognized mAb 109d6 that is also encoded the DRw53 2 gene. Comparison both genes revealed their third D regions (amino acids 67 73) were identical. This suggested first could be residues region, second, putative conversion...