IRE1α is an endoplasmic reticulum (ER)-located transmembrane RNase that plays a central role in the ER stress response. Upon stress, activated and cleaves specific exon–intron sites mRNA encoding transcription factor X-box-binding protein 1 (XBP1). In addition, previous studies allow us to predict targets several RNAs other than XBP1. fact, we have identified CD59 as cleavage target of IRE1α. However, it not yet clear how recognizes RNAs. To address this question, devised unique method...

Several endoplasmic reticulum (ER)-resident luminal proteins have a characteristic ER retrieval signal, KDEL, or its variants at their C terminus. Our previous work searching EST databases for containing the C-terminal KDEL motif predicted some novel murine proteins, one of which designated JPDI (J-domain-containing protein disulfide isomerase-like protein) is characterized in this study. The primary structure unique, because addition to J-domain adjacent N-terminal translocation signal...

Abstract This study replicates and extends the findings of Zerin et al. regarding three‐level systematic FEM thermal stress simulation Ngoc on an effective stepwise construction strategy in context cracking mitigation durable RC deck slabs multi‐span steel girder bridges. The replications demonstrate overall robustness original results increase confidence utilizing stresses for a more comprehensive evaluation early‐age transverse risk highly slabs. Furthermore, countermeasures proposed this...

Endoplasmic reticulum (ER) stress is associated with the functional disorder of ER. During conditions ER stress, cells induce at least two responses to maintain function: transcriptional upregulation quality control genes, and translational attenuation protein synthesis. Induction proteins mediated by IRE1α, which activates transcription factor XBP1 via an unconventional splicing event, while a partial IRE1β. Here, we show both in vivo vitro analyses that RNase domain IRE1 determines...

The 2011 Tohuku tsunami on the east coast of Japan resulted in killing more than 15,000 people and missing 2,500 people, washing away 250 coastal bridges loss US$235 billion. Collapse due to impact represents a huge obstacle for rescue works. Therefore, current study, collapse Utatsu Ohashi bridge is numerically studied. analysis carried out using Applied element Method its advan-tages simulating structural progressive collapse. AEM discrete crack approach, which elements can be separated,...

In March 2011, the Tohoku tsunami swept over east coast of Japan killing more than 15,000 people and missing 2,500 people. The resulted in collapsing 400,000 buildings washing away 250 coastal bridges. this study, collapse Tsuyagawa bridge damaged by is numerically investigated using applied element method (AEM) because its advantages for simulating structural progressive collapse. AEM a discrete crack approach that allows elements separation, fall, collision with each other fully nonlinear...

Road infrastructure has witnessed incremental changes in the past as compared to immense development by vehicle’s safety technology. Bott’s dots and other reflector devices are extensively used on road for lane separation improving edge detection. These come a large variety of shapes sizes, however, all them fall under category retroreflectivity since they depend vehicle lights provide reflection. Glow-in-the-dark (GiD) material benefit that it can store energy during presence light emit...

In eukaryotic cells, most membrane and secretory proteins are modified post-translationally in the ER (endoplasmic reticulum) for correct folding assembly. Disulfide-bond formation is one of important modifications affecting catalysed by PDI (protein disulfide isomerase) family proteins. ERdj5 [also known as JPDI (J-domain-containing PDI-like protein)] a member has been reported to act reductase ERAD (ER-associated degradation). However, role at whole-body level remains unclear. Therefore...

Microcracks developed considerably in concrete subjected to elevated temperature up around 60°C at early ages, especially low water-to-binder ratio (0.3) concrete. Microcracking was attributed the stresses induced by incompatibility deformation between mortar and aggregate. Differences of coefficients thermal expansion (CTE) coarse aggregate, autogenous shrinkage size aggregate were important factors influencing deterioration. The tensile strength severely affected extent microcracks....

Present study aims on evaluating the seismic performances of existing Masonry Infilled Reinforced Concrete (MIRC) buildings commonly found in many South and Southeast Asian countries utilizing appropriate masonry properties Applied Element Method (AEM) models. First, constituents composite were deter-mined for different meshes through extensive parametric studies verified experimental results prisms under uniaxial compression half scaled infilled RC frames in-plane cyclic load. Next,...

完全非破壊で新設および既設コンクリート構造物に適用でき,注水初期から連続的に精度よく吸水量を測定することを特徴とする表面吸水試験手法を開発した.本手法では10分間の測定により吸水抵抗性に関する複数の指標が得られ,深さ方向の影響も含んだ表層品質を評価できる.材料と養生,および暴露環境を変化させた供試体実験からは,水セメント比および型枠存置期間が表層品質に与える影響を表面吸水試験で検知できることを示した.実構造物の測定結果からは,10分時点での表面吸水速度は正規分布をとることが示され,表面吸水試験を表層品質の検査に用いる場合のしきい値の設定方法と検査の判定の考え方を統計的根拠に基づいて提案した.表面吸水試験を用いて微細ひび割れの影響を分析した事例や,膨張材,表面含浸材の効果を検証した事例を紹介した.

The objective of this research is to establish proper modeling FEM simulation for early thermal stress in second lining concrete NATM tunnels. proposed model was verified by field measurements. Stresses at the crown and sides close invert were investigated using established model. First, a scheme considering behaviors detail sufficiently tunnel monitoring data obtained from previous study. It observed that accurate indispensable variation air temperature properties waterproofing membrane...

To establish an optimum surface protection system with silane type water repellents, experimental investigations were conducted for 2 kinds of repellents that had shown good absorption controlling effect in a previous exposure test actual structure carried out by the authors. The effects age application and curing conditions before after on penetration depth investigated concretes different mix proportions four W/C, 35%, 42%, 50%, 65%. Furthermore, specimens used tests subjected to...

The precipitates in cracks an actual utility concrete pole exhibiting self healing which had been service for 34 years a freezing and thawing environment were analyzed the mechanism of was discussed. CaCO3 observed at surface pole, it guessed from condition precipitation that water accumulated inside flowed through crack to surface. ettringite precipitated pole. While approximately 200 μm wide not completely healed, with smaller width 50 100 blocked up precipitates. Precipitates mainly near...