A5037642118- Pancreatic function and diabetes
- Endoplasmic Reticulum Stress and Disease
- Neuroscience and Neuropharmacology Research
- Transgenic Plants and Applications
- Paleontology and Stratigraphy of Fossils
- Neurogenesis and neuroplasticity mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Helicobacter pylori-related gastroenterology studies
- Liver physiology and pathology
- Marine Biology and Ecology Research
- Cell Adhesion Molecules Research
- Asthma and respiratory diseases
- RNA Interference and Gene Delivery
- Biochemical Analysis and Sensing Techniques
- Autophagy in Disease and Therapy
- Erythrocyte Function and Pathophysiology
- Spinal Cord Injury Research
- Cervical Cancer and HPV Research
- Receptor Mechanisms and Signaling
- Olfactory and Sensory Function Studies
- Immunodeficiency and Autoimmune Disorders
- Axon Guidance and Neuronal Signaling
- Amino Acid Enzymes and Metabolism
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
Bioscience Research
2024
Kyoto Pharmaceutical University
2018-2024
The University of Tokyo
1976-2024
Yasuda Women's University
2024
Okayama University
2024
Tohoku University
2013-2024
Kogakuin University
2024
Toyo University
2019-2022
Nara Institute of Science and Technology
2007-2021
Iwate University
2019
In the mouse olfactory system, each sensory neuron (OSN) expresses only one odorant receptor (OR) gene in a monoallelic and mutually exclusive manner. Such expression forms genetic basis for OR-instructed axonal projection of OSNs to bulb brain during development. Here, we identify an upstream cis-acting DNA region that activates OR cluster allows within cluster. Deletion coding expressed or naturally occurring frame-shift mutation second be expressed. We propose stochastic activation...
The body's capacity to restore damaged neural networks in the injured CNS is severely limited. Although various treatment regimens can partially alleviate spinal cord injury (SCI), mechanisms responsible for symptomatic improvement remain elusive. Here, using a mouse model of SCI, we have shown that transplantation stem cells (NSCs) together with administration valproic acid (VPA), known antiepileptic and histone deacetylase inhibitor, dramatically enhanced restoration hind limb function....
In mammals, the prototypical endoplasmic reticulum (ER) stress sensor inositol-requiring enzyme 1 (IRE1) has diverged into two paralogs. IRE1 α is broadly expressed and mediates unconventional splicing of X-box binding protein (XBP1) mRNA during ER stress. By contrast, IRE1β selectively in digestive tract, its function remains unclear. Here, we report that plays a distinctive role mucin-secreting goblet cells. −/− mice, aberrant mucin 2 (MUC2) accumulated cells, accompanied by distension...
Abstract Mammalian inositol-requiring enzyme 1α (IRE1α) is the most conserved of all endoplasmic reticulum (ER) stress sensors, which includes activating transcription factor (ATF) 6 and double-stranded RNA-dependent protein kinase (PKR)-like ER (PERK). IRE1α has been known to splice X-box binding 1 (XBP1) mRNA, induced by ATF6 under stress. This spliced XBP1 mRNA translated into active that promotes expression specific genes alleviate Herein, we report in addition induction ATF6, ATF4,...
In mammalian pancreatic β cells, the IRE1α–XBP1 pathway is constitutively and highly activated under physiological conditions. To elucidate precise role of this pathway, we constructed cell–specific Ire1α conditional knockout (CKO) mice established insulinoma cell lines in which was deleted using Cre–loxP system. CKO showed typical diabetic phenotype including impaired glycemic control defects insulin biosynthesis postnatally at 4–20 weeks. deletion cells resulted decreased secretion,...
Growth cones navigate axonal projection in response to guidance cues. However, it is unclear how they can decide the migratory direction by transducing local spatial cues into protrusive forces. Here we show that knockout mice of Shootin1 display abnormal forebrain commissural axons, a phenotype similar axon molecule netrin-1. Shallow gradients netrin-1 elicited highly polarized Pak1-mediated phosphorylation shootin1 within growth cones. We demonstrate netrin-1–elicited increases interaction...
The major surface glycoprotein (MSG) of Pneumocystis carinii plays a crucial role in the pathobiology P. carinii, which often causes fatal pneumonia AIDS patients. cDNAs encoding MSG antigens were cloned from λgtl1 expression library rat-derived by immunoscreening. constituted gene family containing ∼70% amino acid identity between subtypes. diversity was high and reflected genomic structure genes clustered chromosomes. These multiple may account for high-level ofMSG that could generate...
Abstract ESCs are a potential cell source for therapy. However, there is no evidence that transplantation using ESC-derived hepatocytes therapeutically effective. The main objective of this study was to assess the therapeutic efficacy endodermal cells into liver injury model. β-galactosidase-labeled mouse were differentiated α-fetoprotein (AFP)-producing cells. AFP-producing or transplanted transgenic mice expressed diphtheria toxin (DT) receptors under control an albumin enhancer/promoter....
Arsenic contamination is a major environmental issue, as it may lead to serious health hazard. The reduced trivalent form of inorganic arsenic, arsenite, in general more toxic plants compared with the fully oxidized pentavalent arsenate. uptake arsenite has been shown be mediated through large subfamily plant aquaglyceroporins, nodulin 26-like intrinsic proteins (NIPs). However, efflux mechanisms, well mechanism arsenite-induced root growth inhibition, remain poorly understood. Using...
Neurons sort out a variety of functional molecules to appropriate subcellular destinations. Telencephalin (TLCN; intercellular adhesion molecule-5) is cell molecule specifically localized somatodendritic membranes in the telencephalic neurons. Here, we established new vivo strategy analyze neuronal sorting mechanisms by ectopic expression interest cerebellar Purkinje cells transgenic mice. By using this system, identified novel dendritic targeting determinant cytoplasmic tail region TLCN. A...
Misfolded proteins in the endoplasmic reticulum (ER) are dislocated out of ER to cytosol, polyubiquitinated, and degraded by ubiquitin-proteasome system a process collectively termed ER-associated degradation (ERAD). Recent studies have established that mammalian ER-localized transmembrane J-protein, DNAJB12, cooperates with Hsc70, cytosolic Hsp70 family member, promote ERAD misfolded membrane proteins. Interestingly, genomes another J-protein called DNAJB14 shows high sequence similarity...
Mast cell stabilizers, including disodium cromoglycate (DSCG), were found to have potential as the agonists of an orphan G protein–coupled receptor, GPR35, although it remains be determined whether GPR35 is expressed in mast cells and involved suppression degranulation. Our purpose this study verify expression clarify how modulates We explored roles using system, a line constitutively expressing rat peritoneal cells, bone marrow–derived cultured cells. Immediate allergic responses assessed...
Journal Article A Diphtheria Toxin Receptor Deficient in Epidermal Growth Factor–Like Biological Activity Get access Norihisa Furukawa, Furukawa †To whom correspondence should be addressed at: Laboratory of Molecular and Cell Genetics, Graduate School Sciences, Nara Institute Science Technology (NAIST), 8916-5 Ikoma, 630-0192. Tel: +81-743-72-5640, Fax: +81-743-72-5649, E-mail: kkouno@bs.naist.jp Search for other works by this author on: Oxford Academic PubMed Google Scholar Michiko Saito,...
Abstract A set of genes in the posterior end developing mouse embryos shows oscillatory expression, thereby regulating periodic somite segmentation. Although mechanism for generating oscillation has extensively been clarified, what regulates period is still unclear. We attempted to elongate by increasing time transcribe Hes7 this research. generated knock-in mice, which a large intron was inserted into 3′UTR. The exogenous unexpectedly not properly spliced out and transcripts were...
Early embryogenesis of the two-spotted cricket Gryllus bimaculatus was examined by scanning electron microscopy and several fluorescence staining methods, with special reference to these four issues: (i) location micropyles; (ii) transfer female pronucleus following meiosis; (iii) timing cellularization; (iv) process germ primordium formation. Between two micropyles lie in mid-ventral region egg. The egg nucleus is at mid-dorsal periphery new laid egg, meiosis resumes completed there. moves...
Cell ablation technology is useful for studying specific cell lineages in a developing organ vivo . Herein, we established novel anti-Müllerian hormone ( AMH )-toxin receptor-mediated knockout (Treck) mouse line, which the diphtheria toxin DT ) receptor was specifically activated Sertoli and granulosa cells postnatal testes ovaries respectively. In of Amh -Treck transgenic (Tg) male mice, injection induced loss dose-dependent manner, as well degeneration primary secondary follicles caused by...
IRE1α plays an important role in the unfolded protein response (UPR), which is activated by accumulation of proteins endoplasmic reticulum. 4μ8C, a well-known inhibitor RNase activity, commonly used to analyze function during ER stress cultured mammalian cells. However, off-target effects 4μ8C remain elusive. Pancreatic β-cells synthesize large amount insulin high glucose stimulation, and secretion from pancreatic β-cells. Here, β-cells, we examined after treatment. Although inhibited within...