A5047706295- Developmental Biology and Gene Regulation
- Congenital heart defects research
- Rice Cultivation and Yield Improvement
- Glaucoma and retinal disorders
- Corneal surgery and disorders
- GABA and Rice Research
- Cellular Mechanics and Interactions
- Pluripotent Stem Cells Research
- Intraocular Surgery and Lenses
- Zebrafish Biomedical Research Applications
- Food composition and properties
- Ocular Surface and Contact Lens
- Histone Deacetylase Inhibitors Research
- RNA Research and Splicing
- Retinal and Macular Surgery
- Wnt/β-catenin signaling in development and cancer
- Retinal Diseases and Treatments
- Circadian rhythm and melatonin
- Nuclear Receptors and Signaling
- Sirtuins and Resveratrol in Medicine
- Genetics, Aging, and Longevity in Model Organisms
- Gene Regulatory Network Analysis
- Pancreatic and Hepatic Oncology Research
- Phytochemical Studies and Bioactivities
- Genetic Mapping and Diversity in Plants and Animals
Kanagawa Dental University
2023-2024
Niigata Agricultural Research Institute
2003-2023
Nara Institute of Science and Technology
2013-2022
Nagashima Hospital
2005-2017
Niigata University
2013
Japan Science and Technology Agency
2009-2011
Salk Institute for Biological Studies
2004-2009
Gene Therapy Laboratory
2006
University of Miami
1995-1999
Fukuoka University
1995-1999
ES cells represent a valuable model for investigating early embryo development and hold promise future regenerative medicine strategies. The self-renewal of pluripotent mouse has been shown to require extrinsic stimulation by the bone morphogenetic protein (BMP) leukemia inhibitory factor signaling pathways expression transcription factors Oct4 Nanog. However, network interactions among intrinsic determinants cell pluripotency is currently poorly understood. Here, we show that Nanog...
Initiation and maintenance of signaling centers is a key issue during embryonic development. The apical ectodermal ridge, specialized epithelial structure source Fgf8, pivotal center for limb outgrowth. We show that two closely related buttonhead-like zinc-finger transcription factors, Sp8 Sp9, are expressed in the AER, regulate Fgf8 expression Embryological genetic analyses have revealed Sp8and Sp9 targets Fgf10 from mesenchyme. also found Wnt/β-catenin positively regulates Sp8, but not...
Several components of noncanonical Wnt signaling pathways are involved in the control convergence and extension (CE) movements during zebrafish Xenopus gastrulation. However, complexity these wide patterns expression activity displayed by some their immediately suggest additional morphogenetic roles beyond CE. Here we show that key modular intracellular mediator Dishevelled, through a specific activation RhoA GTPase, controls process endoderm organ precursors toward embryonic midline embryo....
Vertebrate segments called somites are generated by periodic segmentation of the anterior extremity presomitic mesoderm (PSM). During somite in zebrafish, mesp-b determines a future boundary at position B-2 within PSM. Heat-shock experiments, however, suggest that an earlier exists B-5, but molecular signature this remains unidentified. Here, we characterized fibroblast growth factor (FGF) signal activity PSM, and demonstrated limit downstream Erk corresponds to B-5 boundary. Moreover, clock...
The assembly of progenitor cells is a crucial step for organ formation during vertebrate development. Kupffer's vesicle (KV), key required the left–right asymmetric body plan in zebrafish, generated from cluster ∼20 dorsal forerunner (DFCs). Although several genes are known to be involved KV formation, how DFC clustering regulated and then contributes remain unclear. Here we show that positive feedback regulation FGF signaling by Canopy1 (Cnpy1) controls clustering. Cnpy1 positively...
Little is known about how the sizes of animal tissues are controlled. A prominent example somite size which varies widely both within an individual and across species. Despite intense study segmentation clock governing timing generation, it relates to poorly understood. Here we examine scaling find that at specification scales with length presomitic mesoderm (PSM) despite considerable variation in PSM developmental stages surgically size-reduced embryos. Measurement period, axis elongation...
ATP1A3 , a mutated gene for AHC, RDP, and CAPOS, is also associated with polymicrogyria by different functional variants.
The number of vertebrae is defined strictly for a given species and depends on the somites, which are earliest metameric structures that form in development. Somites formed by sequential segmentation. periodicity somite segmentation orchestrated synchronous oscillation gene expression presomitic mesoderm (PSM), termed "somite clock," Notch signaling plays crucial role. Here we show clock period sensitive to activity, fine-tuned its feedback regulator, Notch-regulated ankyrin repeat protein...
During somite segmentation, clock genes oscillate within the posterior presomitic mesoderm (PSM). The temporal information ties up with posteriorly moving FGF gradient, leading to formation of a presumptive PSM. We previously investigated Erk activity downstream signaling by collecting stained zebrafish embryos, and discovered that steep gradient was generated in PSM, border regularly shifted stepwise manner. However, since these interpretations come from static analyses, we needed firmly...
Oryza sativa L., blast resistance, breeding, isogenic line, Koshihikari, multiline variety, rice variety
Background During vertebrate embryogenesis, somites are generated at regular intervals, the temporal and spatial periodicity of which is governed by a gradient fibroblast growth factor (FGF) and/or Wnt signaling activity in presomitic mesoderm (PSM) conjunction with oscillations gene expression components Notch, FGF pathways. Principal Findings Here, we show that Sprouty4, encodes an inhibitor, oscillates 2-h cycles mouse PSM synchrony other oscillating genes from Notch pathway, such as...
Senescent cells accumulate in tissues of aged animals and deteriorate tissue functions. The elimination senescent from mice not only attenuates progression already established age‐related disorders, but also extends median lifespan. Nicotinamide phosphoribosyltransferase ( NAMPT ), the rate‐limiting enzyme mammalian NAD + salvage pathway, has shown a protective effect on cellular senescence human primary cells. However, it still remains unclear how impact aging vitro vivo. In this study, we...
During vertebrate development, extracellular signal-regulated kinase (ERK) is activated by growth factors such as fibroblast factor (FGF), and it regulates the formation of tissues/organs including eyes, brains, somites, limbs, inner ears. However, an experimental system to monitor ERK activity dynamics in entire body embryo lacking. We recently studied pre-somitic mesoderm living zebrafish embryos injected with mRNAs encoding a Förster resonance energy transfer (FRET)-based biosensor. In...
Over the last decade, a wide array of evidence has been accumulated that disruption circadian clock is prone to cause age-related diseases and premature aging. On other hand, aging identified as one risk factors linked alteration clock. These evidences suggest processes feedback on each at animal level. However, cellular level, we recently revealed primary fibroblast cells derived from Bmal1-/- mouse embryo, in which completely disrupted, do not demonstrate acceleration aging, i.e.,...
The circadian clock possesses robust systems to maintain the rhythm approximately 24 h, from cellular organismal levels, whereas aging is known be one of risk factors linked alternation physiology and behavior. amount many metabolites in cells/body altered with process, most prominent metabolite among them oxidized form nicotinamide adenine dinucleotide (NAD + ), which associated posttranslational modifications acetylation poly-ADP-ribosylation status proteins decreases aging. However, how...
Abstract A set of genes in the posterior end developing mouse embryos shows oscillatory expression, thereby regulating periodic somite segmentation. Although mechanism for generating oscillation has extensively been clarified, what regulates period is still unclear. We attempted to elongate by increasing time transcribe Hes7 this research. generated knock-in mice, which a large intron was inserted into 3′UTR. The exogenous unexpectedly not properly spliced out and transcripts were...
Somite segmentation, a prominent periodic event in the development of vertebrates, is instructed by cyclic expression several genes, including Hes7 and Lunatic fringe (Lfng). Transcriptional regulation accounts for expression. In addition, because patterns vary cycle, rapid turnover mRNAs should be involved expression, although its contribution remains unclear. Here, we demonstrate that 3'-UTR-dependent Lfng plays critical role their dynamic patterns. The regions active transcription are...
The incidence of non-alcoholic steatohepatitis (NASH)-related hepatocellular carcinoma (HCC) is increasing annually as the metabolic syndrome factors increase. This study aimed to analyze involvement periodontopathic bacteria in NASH-related HCC (NASH-HCC). Questionnaire investigation, periodontal examination, medical and specimen collection (saliva, mouth-rinsed water, peripheral blood) were performed 40 patients with NASH 20 NASH-HCC. Immunoglobulin (Ig) G antibody titers against...