A5082870137- Pediatric Hepatobiliary Diseases and Treatments
- Nicotinic Acetylcholine Receptors Study
- Cholinesterase and Neurodegenerative Diseases
- Infant Nutrition and Health
- Liver physiology and pathology
- Pancreatic function and diabetes
- Neuroscience and Neuropharmacology Research
- Anesthesia and Neurotoxicity Research
- Congenital Anomalies and Fetal Surgery
- Urticaria and Related Conditions
- Sperm and Testicular Function
- Alcoholism and Thiamine Deficiency
- Epigenetics and DNA Methylation
- Acute Myeloid Leukemia Research
- Dermatology and Skin Diseases
- Osteoarthritis Treatment and Mechanisms
- Spondyloarthritis Studies and Treatments
- Neonatal Health and Biochemistry
- Surgical Simulation and Training
- Nerve injury and regeneration
- Retinoids in leukemia and cellular processes
- Bee Products Chemical Analysis
- Vitamin K Research Studies
- Macrophage Migration Inhibitory Factor
- Systemic Sclerosis and Related Diseases
Azabu University
2011-2024
The University of Tokyo
2012-2017
Pharmaceuticals and Medical Devices Agency
2016
Tokyo Medical and Dental University
2014
Congenital biliary atresia is an incurable disease of newborn infants, unknown genetic causes, that results in congenital deformation the gallbladder and duct system. Here, we show during mouse organogenesis, insufficient SOX17 expression bile epithelia subsequent acute 'embryonic hepatitis', leading to perinatal death ~95% Sox17 heterozygote neonates C57BL/6 (B6) background mice. During development, was expressed at distal edge primordium. In Sox17(+/-) B6 embryos, were hypoplastic, some...
Abstract Vitamin K (VK) has a protective effect on neural cells. Methylmercury is neurotoxicant that directly induces neuronal death in vivo and vitro . Therefore, the present study, we hypothesized VK inhibits neurotoxicity of methylmercury. To prove our hypothesis , investigated effects VKs (phylloquinone, vitamin 1 ; menaquinone‐4, 2 ) methylmercury‐induced primary cultured neurons from cerebella rat pups. As expected, inhibited neurons. It been reported mechanisms underlying...
The gallbladder excretes cytotoxic bile acids into the duodenum through cystic duct and common system. Sox17 haploinsufficiency causes biliary atresia-like phenotypes hepatitis in late organogenesis mouse embryos, but molecular cellular mechanisms underlying this remain unclear. In study, transcriptomic analyses revealed early onset of cholecystitis Sox17+/− together with appearance ectopic duct-like epithelia their gallbladders. embryonic showed positive correlations severity individual...
The biliary tract is a well-branched ductal structure that exhibits great variation in morphology among vertebrates. Its function maintained by complex constructions of blood vessels, nerves, and smooth muscles, the so-called hepatobiliary system. Although mouse (Mus musculus) has been used as model organism for humans, its system not well documented at topographical level, mostly because small size complexity. To reconcile this, we conducted whole-mount anatomical descriptions murine...
Cell ablation technology is useful for studying specific cell lineages in a developing organ vivo . Herein, we established novel anti-Müllerian hormone ( AMH )-toxin receptor-mediated knockout (Treck) mouse line, which the diphtheria toxin DT ) receptor was specifically activated Sertoli and granulosa cells postnatal testes ovaries respectively. In of Amh -Treck transgenic (Tg) male mice, injection induced loss dose-dependent manner, as well degeneration primary secondary follicles caused by...
In early embryogenesis, the posteroventral foregut endoderm gives rise to budding endodermal organs including liver, ventral pancreas and gallbladder during somitogenesis. Despite detailed fate maps of liver pancreatic progenitors in mouse endoderm, exact location remains unclear. this study, we performed a DiI fate-mapping analysis using whole-embryo cultures somite-stage embryos. Here, show that majority 9–11-somite-stage embryos are located lateral-most domain at first intersomite...
Brain-derived neurotrophic factor (BDNF) is a principal for neurogenesis, neurodevelopment and neural survival through BDNF receptor, tropomyosin-related kinase (Trk) B, while can also cause decrease in the intracellular glutathione (GSH) level. We investigated exacerbation of methylmercury-induced death rat cerebellar granular neurons (CGNs) by vitro. Since methylmercury GSH levels, we hypothesized that further level involved process neuronal cell death. In present study, established CGN...
Hepatic stellate cells (HSCs) intracellularly preserve vitamin A in the normal liver. When liver is damaged, HSCs transform into myofibroblast-like cells, and then proliferate increase their expression of collagen. Cultured on a plastic plate, spontaneously activate. To maintain quiescent state with low collagen, coating methods extracellular matrixes (ECMs) such as Matrigel-coating or laminin-rich are commonly used for HSC cultivation. Kishimoto et al. [14] reported that Fragmin®/protamine...
Three-dimensional (3D)-printed models of bones are a convenient and durable alternative to real bone specimens, they have been used in anatomy laboratories. It is necessary identify the precise advantages 3D-printed from all perspectives; not only improvement students' knowledge but also assessment such models. Here, students veterinary medicine animal science evaluated reproducibility effectiveness as learning tool by completing our questionnaires, with focus on their understanding...
The regulation of glial cells, especially astrocytes and microglia, is important to prevent the exacerbation a brain injury because over-reactive cells promote neuronal death. Acetylcholine (ACh), neurotransmitter synthesized hydrolyzed by choline acetyltransferase (ChAT) acetylcholinesterase (AChE), respectively, in central nervous system, has potential regulate cells' states, i.e., non-reactive reactive states. However, expression levels these ACh-related enzymes areas containing are...