A5102024462- Pancreatic function and diabetes
- Endoplasmic Reticulum Stress and Disease
- Pediatric Hepatobiliary Diseases and Treatments
- Renal Transplantation Outcomes and Treatments
- Pharmacological Effects and Toxicity Studies
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Organ Transplantation Techniques and Outcomes
- Gallbladder and Bile Duct Disorders
- Antibiotics Pharmacokinetics and Efficacy
- Colorectal and Anal Carcinomas
- Transplantation: Methods and Outcomes
- Genetic factors in colorectal cancer
- Epilepsy research and treatment
- Adenosine and Purinergic Signaling
- Burn Injury Management and Outcomes
- Cleft Lip and Palate Research
- Transgenic Plants and Applications
- Pancreatitis Pathology and Treatment
- Liver physiology and pathology
- Multiple and Secondary Primary Cancers
- Drug-Induced Hepatotoxicity and Protection
- Injury Epidemiology and Prevention
- Hematopoietic Stem Cell Transplantation
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Cytomegalovirus and herpesvirus research
Kyushu University Hospital
2016-2024
Kyushu University
2024
Saiseikai Utsunomiya hospital
2022
Rockefeller University
2019
Nara Institute of Science and Technology
2016-2018
Tokyo National Hospital
1996
Osaka National Hospital
1990-1991
In mammalian pancreatic β cells, the IRE1α–XBP1 pathway is constitutively and highly activated under physiological conditions. To elucidate precise role of this pathway, we constructed cell–specific Ire1α conditional knockout (CKO) mice established insulinoma cell lines in which was deleted using Cre–loxP system. CKO showed typical diabetic phenotype including impaired glycemic control defects insulin biosynthesis postnatally at 4–20 weeks. deletion cells resulted decreased secretion,...
Nirmatrelvir/ritonavir is a treatment for COVID-19 consisting of nirmatrelvir, which has anti-SARS-CoV-2 activity, and ritonavir, booster to maintain blood levels. Ritonavir known be potent inhibitor cytochrome P450 3A (CYP3A), interactions with CYP3A-metabolized drugs, such as the immunosuppressant tacrolimus, can problematic. Ritonavir's inhibition CYP3A irreversible due covalent binding, its inhibitory effects are expected persist until replaced by new CYP3A. Here, we report case where...
IRE1α plays an important role in the unfolded protein response (UPR), which is activated by accumulation of proteins endoplasmic reticulum. 4μ8C, a well-known inhibitor RNase activity, commonly used to analyze function during ER stress cultured mammalian cells. However, off-target effects 4μ8C remain elusive. Pancreatic β-cells synthesize large amount insulin high glucose stimulation, and secretion from pancreatic β-cells. Here, β-cells, we examined after treatment. Although inhibited within...
Therapeutic drug monitoring of tacrolimus is necessary for appropriate dose adjustment a successful immunosuppressive therapy. Several commercial immunoassays are available measurements. This study aimed at simultaneously evaluating the analytical performances 4 such immunoassays, using liquid chromatography-tandem mass spectrometry (LC-MS/MS) as standard. For first time, cross-reactivity to metabolites was assessed concentrations frequently observed in clinical settings, opposed higher...
Perampanel (PER) is an oral antiepileptic drug and its concomitant use with carbamazepine (CBZ) leads to decreased PER concentrations. However, the magnitude of influence may vary, depending on dynamics enzyme induction properties CBZ. This study aimed develop a population pharmacokinetic (PPK) model considering evaluate effect CBZ pharmacokinetics.We retrospectively collected data patient background, laboratory tests, prescribed drugs from electronic medical records. We developed 2 PPK...
With the aim of studying pharmacokinetics letermovir, which is a newly developed antiviral agent for human cytomegalovirus, rapid and simple ultra-performance liquid chromatography coupled with mass spectrometry (UPLC/MS) method was validated quantification letermovir in plasma. Separation performed reverse phase mode using an ACQUITY UPLC BEH C18 column (130 Å, 1.7 µm, 2.1 × 50 mm) at flow rate 0.3 mL/min, 10 mM ammonium acetate–0.1% formic acid solution as mobile A, acetonitrile B gradient...
We proposed a novel ligand for the interaction with human serum albumin (HSA) to extend blood half-life of small molecular weight therapeutics. The features an alkyl chain and activated disulfide allow binding hydrophobic pockets HSA formation Cys34 HSA, thereby minimizing initial renal clearance. dual nature ligand–HSA bonding was expected give long retention. After 1 min mixing showed higher (1.7 times) than that control (containing only disulfide). intravenous injection mice, half-lives...
Background: Gilteritinib, a novel oral tyrosine kinase inhibitor, is used to treat acute myeloid leukemia (AML) with FMS-like kinase-3 (FLT3) mutations. Therapeutic drug monitoring (TDM) of gilteritinib important for improving clinical outcomes and ensuring safety. Therefore, this study aimed develop simplified method quantifying in human plasma using liquid chromatography–tandem mass spectrometry. Methods: Liquid chromatography was performed by an Acquity BEH C18 column (50 mm × 2.1 mm, 1.7...
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This is a report of three cases using the Y-shaped anastomotic technique. anastomoses were found to be extremely useful in selected clinical situations for free flap transfers.
インスリンは，血糖値の上昇に応じて血中へと分泌され，糖の取り込みと糖代謝を促す重要なホルモンで膵臓ランゲルハンス島のβ細胞で合成される．インスリンの分泌や合成が低下すると，高血糖が持続する糖尿病を発症することが知られている．したがって，膵臓β細胞で，インスリン合成がどのように行われ，生理的にどのように正確に制御されているのかを理解することは，インスリン合成の観点からだけでなく，糖尿病の予防および治療の観点からも非常に重要である．インスリン前駆体のプロインスリンは，小胞体と呼ばれる細胞小器官で合成され，3つのジスルフィド結合形成を介して酸化的に正しく折り畳まれる．これまで，プロインスリンの折り畳み酵素やそれらの酵素群の発現制御の機構は未解明のままであった．今回，私たちは遺伝子改変マウスと膵臓由来β培養細胞の解析により，小胞体ストレスセンサーIRE1αが膵臓β細胞で5つの酵素遺伝子を転写誘導することで，プロインスリンの折り畳みを促進していることを見いだした(1)．
For many of the novel antiepileptics, immunoassays, used for routine therapeutic drug monitoring (TDM), cannot be used. We could monitor eight antiepileptics using an LC/MS method since July 2017. The purpose this study was to evaluate significant changes associated with transition from outsourcing in-hospital antiepileptics. number measurements significantly increased during first (p<0.01) and second (p<0.001) years as compared that one year prior which outsourced. proportion all 19.7%,...
Background: It is well known that the possibility of abuse must be considered when examining pediatric burns; however, in reality, many these are accidental injuries. Some burns due to lack supervision or childcare, which should negligence.Methods: We reviewed age, department at first consultation, and social worker intervention from medical records 189 burn patients aged < 12 years between January 2016 2019.Results: Twenty-seven percent (50 children) were 12–23 months. The most common...
直腸pm癌の原発巣の病理組織学的所見がどの程度予後に反映されているかを検討した.1975年から1988年の間に当院で手術を行った直腸pm癌62例を対象とした.転移再発した14例を予後不良群-A群-, 転移再発を認めない48例を予後良好群-B群-として2群を比較した.A群では高分化型腺癌3例, 中分化型腺癌11例であり, B群ではそれぞれ29例, 19例であった.固有筋層への浸潤程度pm1, pm2, pm3に分けるとA群ではそれぞれ2例, 4例, 8例, B群ではそれぞれ18例, 19例, 11例であった.静脈侵襲陽性率はA群で71.4%, B群で35.4%, リンパ節転移陽性率はA群で64.3%, B群で12.5%であった.これらについては2群間に有意差を認めたが, INFとリンパ管侵襲は, 2群間に有意差を認めなかった.以上の結果より, 直腸pm癌においては癌の分化度, 固有筋層への浸潤程度, 静脈侵襲, リンパ節転移が予後を推測する上で重要な因子であることが示唆される.
線毛運動異常症 (ICS) の20歳女性と嚢胞性線維症 (CF) の11歳男子における肺緑膿菌感染 増悪の治療経験を比較検討した。ICS例は息切れ・発熱を主訴とし, 著明な発熱・炎症反応亢進・胸部レントゲンにおける肺野の浸潤影を特徴とした。これに対して, CF例は息切れ・排疾困難を主訴 とし, 発熱を伴わず呼吸困難が強かった。炎症反応は中等度陽性であり, 胸部レントゲンでは肺門 付近の陰影を主体としていた。気管支拡張症における肺緑膿菌感染増悪の形態は, 肺炎を主体とする型と, 喀疾による気道閉塞 を主体とする型があると考えられ, 肺炎型では抗生剤・ガンマグロブリン療法等が治療の中心であ るが, 気道閉塞型では抗生剤投与以外に理学療法・吸入療法による排疾促進が重要であった。