A5082939824- Cancer, Hypoxia, and Metabolism
- Advanced Biosensing Techniques and Applications
- Epigenetics and DNA Methylation
- Mass Spectrometry Techniques and Applications
- Nanopore and Nanochannel Transport Studies
- Bioinformatics and Genomic Networks
- Microfluidic and Capillary Electrophoresis Applications
- Estrogen and related hormone effects
- Healthcare and Environmental Waste Management
Al-Zaytoonah University of Jordan
2021-2023
Lactate dehydrogenase (LDH) is a key enzyme in the last step of glycolysis, playing role pyruvate-to-lactate reaction. It associated with prognosis and metastasis many cancers, including breast cancer. In this study, we investigated changes LDH gene expression lactate concentrations culture media during tamoxifen resistance development MCF-7 cell line, examined LDHB promoter methylation levels. An upregulation 2.9 times was observed around IC50 concentration treated cells, while fluctuation...
Breast cancer is one of the most prevalent cancers worldwide usually treated with Tamoxifen. Tamoxifen resistance development challenging issue in an initially responsive breast tumor, and mechanisms are still under investigation. The objective this study to develop validate a selective, sensitive, simultaneous high performance liquid chromatography–tandem mass spectrometry method explore changes substrates metabolites supernatant media developed MCF-7 cells. We focus on determination...
Breast cancer is the second leading cause of death in women after lung cancer. The first-line treatment metastatic breast premenopausal relies on tamoxifen. development tamoxifen resistance not fully understood. In this study, capillary electrophoresis with capacitively coupled contactless conductivity detector was developed to monitor changes lactate and pyruvate levels supernatant media three models MCF-7 tamoxifen-resistant cells correlate these metabolites dehydrogenase genes expression...
The rate of smokers in Jordan has been among the highest globally. Electronic Nicotine Delivery systems (ENDs) are considered helpful smoking cessation but also have potential for metals exposure resulting from their transfer metallic coils to e-liquid upon use. Metal is associated with severe health outcomes. We sought assess levels toxic (Cr, Cd, Pb, Ni and Al) two most popular used ENDs users Jordan. validated inductively coupled plasma-optical emission spectroscopy (ICP-OES) limit...