One-step fabrication of transparent three-dimensional (3D) microfluidic to millifluidic devices was demonstrated using a commercial 3D printer costing $2300 with 500 mL clear resin for $138. It employs dynamic mask projection stereolithography, allowing fast concept-to-chip time. The fully automated system allows models up 43 mm × 27 180 (x y z) at printing speeds 20 mm/h in height regardless the design complexity. minimal cross sectional area 250 μm achieved monolithic microchannels and 200...

Lubricin is a glycoprotein found in articular joints which has long been recognized as being an important biological boundary lubricant molecule and, more recently, impressive antiadhesive that readily self-assembles into well ordered, polymer brush layer on virtually any substrate. The lubricin possesses overabundance of anionic charge, property atypical among molecules, enables its use coating for applications involving electrokinetic processes such electrophoresis and electroosmosis....

Tobacco smoking is a preventable main cause of fatal diseases. Accurate measurements the effects it has on neurotransmitters are essential in developing new strategies for cessation. Moreover, neurotransmitter levels can aid drugs that counteract smoking. The aim this study to develop and validate fast, simultaneous sensitive method measuring rat brain after exposure tobacco cigarettes. selected include dopamine, GABA, serotonin, glutamine glutamate. based high-performance liquid...

Control of the dielectric breakdown PDMS was achieved by limiting current during process. This enabled tuning nanochannel pore size and hence their permeability for molecules different molecular weights. method analysis drug quinine from whole blood in 3 min using a simple, disposable microfluidic device.

Abstract The extraction of target analytes from biological samples is a bottleneck in analysis. A microfluidic device featuring an electrokinetic size and mobility trap was formed by two nanojunctions different pore to extract concentrate analytical targets complex samples. seamlessly coupled with electrophoretic separation for quantitative applied the analysis ampicillin levels blood within 5 min linear response over range 2.5–20 μg mL −1 . This covers recommended treating sepsis, critical...

Breast cancer is one of the most prevalent cancers worldwide usually treated with Tamoxifen. Tamoxifen resistance development challenging issue in an initially responsive breast tumor, and mechanisms are still under investigation. The objective this study to develop validate a selective, sensitive, simultaneous high performance liquid chromatography–tandem mass spectrometry method explore changes substrates metabolites supernatant media developed MCF-7 cells. We focus on determination...

Abstract Thromboembolism because of coronavirus infection (COVID-19) resulted in increasing the number sudden deaths after recovery. So, patients were switched to edoxaban tosylate monohydrate (EDTM) therapy low molecular weight heparin shorten hospitalization period. In response increase demand for EDTM, we developed a home-made screen-printed electrode determine EDTM its pure form and pharmaceutical dosage form. Optimized conditions achieved with utilizing EDTM-Phosphotungstic acid...

Extracellular Ca2+ ([Ca2+]ex) is an important regulator of various physiological and pathological functions, including intercellular communication for synchronized cellular activities (e.g., coordinated hormone secretion from endocrine tissues). Yet it rarely possible to concurrently quantify the dynamic changes [Ca2+]ex related bioactive molecules with high accuracy temporal resolution. This work aims develop a multiplexed microfluidic platform enable monitoring oscillatory hormone(s) in...

High solids content complicates in situ analysis of chemical processing, biological suspensions, and environmental streams. In most cases, analytical methods require at least one pre-treatment step a small volume sample before particle-free fluid can be analyzed. We have developed continuous sampler that "sip" solution from turbulent high stream (a slurry). An open microfluidic chip with an extended slit opening shields the internal laminar flow turbulence outside. Unlike other chips, our...

Breast cancer is the second leading cause of death in women after lung cancer. The first-line treatment metastatic breast premenopausal relies on tamoxifen. development tamoxifen resistance not fully understood. In this study, capillary electrophoresis with capacitively coupled contactless conductivity detector was developed to monitor changes lactate and pyruvate levels supernatant media three models MCF-7 tamoxifen-resistant cells correlate these metabolites dehydrogenase genes expression...

Abstract The extraction of target analytes from biological samples is a bottleneck in analysis. A microfluidic device featuring an electrokinetic size and mobility trap was formed by two nanojunctions different pore to extract concentrate analytical targets complex samples. seamlessly coupled with electrophoretic separation for quantitative applied the analysis ampicillin levels blood within 5 min linear response over range 2.5–20 μg mL −1 . This covers recommended treating sepsis, critical...

Methylglyoxal (MGO) is a genotoxic α-dicarbonyl compound. Recently, it was found to be formed in glycerol preparations during storage through auto-oxidation. A simple fluorimetric determination of the carcinogenic degradation product glycerol, MGO, developed and validated. The proposed method based on derivatization MGO with 4-carbomethoxybenzaldehyde (CMBA) ammonium acetate yield fluorescent imidazole derivative that can measured at 415 nm after excitation 322 nm. optimized conditions were...

Abstract A green spectrofluorimetric method was introduced for the determination of selected genotoxic impurities; 2-aminopyridine and 3-aminopyridine in different pharmaceutical raw materials dosage forms. The relied on native fluorescence these impurities acidic medium. experimental conditions were carefully studied optimized, validated according to International Council Harmonisation (ICH) guidelines. linear range both analytes 2.50–100 ng/mL with good coefficients 0.9995 0.9992 detection...

Abstract A precise, sensitive eco-friendly, simple, rapid, and derivative spectrofluorimetric method was developed to quantify edoxaban tosylate monohydrate in pure form pharmaceutical dosage form. Sudden death due pulmonary embolism as a consequence of coronavirus infection (covid-19) is an emerging problem. As result, the world health organization introduced new guidelines treat patients with COVID-19 oral anticoagulants. Edoxaban anticoagulant that doesn’t require hospitalization after...

Zwei Nanoknotenpunkte unterschiedlicher Porengröße sind die Hauptbestandteile der Größen- und Mobilitätsfallen, M. C. Breadmore Mitarbeiter in ihrer Zuschrift auf S. 7467 ff. beschreiben. Die Fallen wurden für Extraktion, Aufkonzentration Reinigung von Pharmazeutika aus Körperflüssigkeiten vor Auftrennung durch Mikrochip-Elektrophorese entwickelt. Therapeutische Mengen des Antibiotikums Ampicillin konnten Blutproben innerhalb 5 min nachgewiesen werden.

Breast cancer is among the most common types worldwide. The first and second line treatment protocols for various stages of breast in females rely on tamoxifen. Until now, development tamoxifen resistance not entirely understood. In this study, an automated sequential injection capillary electrophoresis with capacitively coupled contactless conductivity detector was developed online levels monitoring both lactate pyruvate from supernatant media MCF-7 cells developing resistance. Changes...

Trace levels of cadmium, lead and mercury heavy metals are toxic to the human nervous system. These occur naturally as a result inappropriate agricultural industrial practices. In response this problem, novel modified electrode was constructed for simultaneous determination mercury. The prepared by incorporating zirconium oxide nanoparticles (ZrO 2 NPs) multi-walled carbon nanotubes (MWCNTs) into paste electrode. Transmission electron microscope electrochemical impedance spectroscopy were...

Abstract High solids content complicates in situ analysis of chemical processing, biological suspensions, and environmental streams. In most cases, analytical methods require at least one pre‐treatment step a small volume sample before particle‐free fluid can be analyzed. We have developed continuous sampler that “sip” solution from turbulent high stream (a slurry). An open microfluidic chip with an extended slit opening shields the internal laminar flow turbulence outside. Unlike other...