One-step fabrication of transparent three-dimensional (3D) microfluidic to millifluidic devices was demonstrated using a commercial 3D printer costing $2300 with 500 mL clear resin for $138. It employs dynamic mask projection stereolithography, allowing fast concept-to-chip time. The fully automated system allows models up 43 mm × 27 180 (x y z) at printing speeds 20 mm/h in height regardless the design complexity. minimal cross sectional area 250 μm achieved monolithic microchannels and 200...

Three-dimensional (3D) printing has emerged as a potential revolutionary technology for the fabrication of microfluidic devices. A direct experimental comparison three 3D technologies dominating microfluidics was conducted using Y-junction device, design which optimized each printer: fused deposition molding (FDM), Polyjet, and digital light processing stereolithography (DLP-SLA). Printer performance evaluated in terms feature size, accuracy, suitability mass manufacturing; laminar flow...

A microchip solid-phase extraction method for purification of DNA from biological samples, such as blood, is demonstrated. Silica beads were packed into glass microchips and the immobilized with sol-gel to provide a stable reproducible solid phase onto which could be adsorbed. Optimization loading conditions established higher recovery at pH 6.1 than 7.6. This lower also allowed flow rate increased, resulting in decrease time 25 min less 15 min. Using this procedure, template genomic human...

A silica-based solid-phase extraction system suitable for incorporation into a microchip platform (ν-total analytical system; ν-TAS) would find utility in variety of genetic analysis protocols, including DNA sequencing. The procedure utilized is based on adsorption the onto bare silica. involves three steps: (i) presence chaotropic salt, (ii) removal contaminants with an alcohol/water solution, and (iii) elution adsorbed small volume buffer polymerase chain reaction (PCR) amplification....

Abstract Poor sensitivity is considered to be one of the major limitations electrophoretic separation methods, particularly when compared traditional liquid chromatographic techniques. To address this issue, various in‐line preconcentration techniques have been developed over past 15 years, ranging in power and complexity, there are now a number well understood approaches routinely capable providing 10 000‐ 100 000‐fold increase sensitivity, as several that can pushed above million....

Abstract Capillary electrophoresis has been alive for over two decades now; yet, its sensitivity is still regarded as being inferior to that of more traditional methods separation such HPLC. As such, it unsurprising overcoming this issue generates much scientific interest. This review continues update series reviews, first published in Electrophoresis 2007, with an 2009 and covers material through June 2010. It includes developments the fields stacking, covering all from field‐amplified...

One of the largest impediments in development microfluidic-based smart sensing systems is manufacturability integrated, complex devices. Here we propose multimaterial 3D printing for fabrication such devices a single step. A microfluidic device containing an integrated porous membrane and embedded liquid reagents was made by applied analysis nitrate soil. The manufacture sealed realized as print within 30 min. body printed transparent acrylonitrile butadiene styrene (ABS) contained 400 μm...

A relative lack of printable materials with tailored functional properties limits the applicability three-dimensional (3D) printing. In this work, a diamond–acrylonitrile butadiene styrene (ABS) composite filament for use in 3D printing was created through incorporation high-pressure and high-temperature (HPHT) synthetic microdiamonds as filler. Homogenously distributed diamond filaments, containing either 37.5 or 60 wt % microdiamonds, were formed preblending powder ABS, followed by...

A commercial portable capillary electrophoresis (CE) instrument has been used to separate inorganic anions and cations found in postblast residues from improvised explosive devices (IEDs) of the type frequently terrorism attacks. The purpose this analysis was identify used. CE modified for use with an in-house miniaturized light-emitting diode (LED) detector enable sensitive indirect photometric detection be employed 15 (acetate, benzoate, carbonate, chlorate, chloride, chlorite, cyanate,...

Abstract Poor sensitivity is still considered to be one of the major limitations electrophoresis, which surprising given power, flexibility and versatility many approaches on‐line concentration that have developed over last 20 years. This a very active area interest this review will cover developments in field two years since ( Electrophoresis 2007, 28, 254–281) through June 2008. It includes fields stacking, covering all methods from field‐amplified sample stacking large volume ITP, dynamic...

Abstract Novel CE methods have been developed on portable instrumentation adapted to accommodate a capacitively coupled contactless conductivity detector for the separation and sensitive detection of inorganic anions cations in post‐blast explosive residues from homemade devices. The presented combine sensitivity speed analysis wide range ions used this study. Separate were employed cations. anion method utilised low 70 mM Tris/70 CHES aqueous electrolyte (pH 8.6) with 90 cm capillary coated...

Multimaterial 3D printing provides a unique capability for the creation of highly complex integrated devices where complementary functionality is realized using differences in material properties. Using single and automated print process, microfluidic were fabricated containing (i) an optically transparent structure fluorescence detection, (ii) electrodes electrokinetic transport, (iii) primary membrane to remove particulates macromolecules including proteins, (iv) secondary concentrate...

Fluidic behavior in microfluidic devices is dictated by low Reynolds numbers, complicating mixing. Here, the effect of orientation extruded filament on fluidic investigated fused deposition modeling (FDM) printed devices. Devices were with orientations at 0°, 30°, 60°, and 90° to direction flow. The extent mixing was observed when pumping yellow blue solutions into inlets a Y-shaped device, measuring two colored under different angles flow rates 25, 50, 100 μL/min. 60° showed highest...

Multimaterial 3D printing facilitates the rapid production of complex devices with integrated materials varying properties and functionality. Herein, multimaterial fused deposition modeling (MM-FDM) was applied to fabrication low-cost passive sampler porous membranes. Using MM-FDM printing, device body produced using black polylactic acid, Poro-Lay Lay-Felt filament used for membranes (rubber-elastomeric polymer, after removal a water-soluble poly(vinyl alcohol) component). The resulting...

Three-dimensional (3D) printing techniques have greatly simplified prototype manufacturing and complex design. However, most commercially available stereolithography (SLA) material components are based on (meth)acrylate-based resin systems that several disadvantages associated with their use, such as inhibition of polymerization by oxygen, solvent resistance, the inability to modify surfaces post printing. Polymerization via a thiol–acrylate mechanism can help overcome many these drawbacks;...