A5087112572- Microfluidic and Capillary Electrophoresis Applications
- Advancements in Transdermal Drug Delivery
- Nicotinic Acetylcholine Receptors Study
- Heavy Metals in Plants
- 3D Printing in Biomedical Research
- Microfluidic and Bio-sensing Technologies
- Cancer, Hypoxia, and Metabolism
- Advanced Biosensing Techniques and Applications
- Neuroscience and Neuropharmacology Research
- Biosensors and Analytical Detection
- Innovative Microfluidic and Catalytic Techniques Innovation
- Heavy metals in environment
- Biochemical Analysis and Sensing Techniques
- Healthcare and Environmental Waste Management
- Drug Solubulity and Delivery Systems
- Epigenetics and DNA Methylation
- Ocular Infections and Treatments
- Gold and Silver Nanoparticles Synthesis and Applications
- Histone Deacetylase Inhibitors Research
- Salivary Gland Disorders and Functions
- Pressure Ulcer Prevention and Management
- Pharmacological Effects of Natural Compounds
- Seaweed-derived Bioactive Compounds
- Phytochemistry and Biological Activities
- Analytical Chemistry and Chromatography
Al-Zaytoonah University of Jordan
2018-2025
King Hussein Medical Center
2024
Jordan University of Science and Technology
2021-2022
University of Benin
2021
University of Tasmania
2013-2017
Abstract Herein, a library of gold nanorods (GNR) decorated with polyethylene glycol-thiol (PEG-SH) containing different functionalities were synthesized and characterized by optical absorption spectroscopy, zeta potential, dynamic light scattering (DLS), transmission electron microscope (TEM) proton nuclear magnetic resonance ( 1 H-NMR). The colloidal stability GNR when exposed to skin, their preferential accumulation into excised human skin layers investigated. Confocal laser scanning...
This study explores the development of buccal films as an innovative drug delivery system for hypertension management, combining candesartan cilexetil (CC) and hydrochlorothiazide (HCTZ). Utilizing a solvent casting technique, were prepared with various polymers, including PVA, PVP, CMC Na, enhanced plasticizers to improve solubility bioavailability. The evaluated physicochemical mechanical properties, disintegration time, content uniformity. Results demonstrated that visually uniform,...
Abstract Tamoxifen resistance is emerging as a big challenge in endocrine therapy of luminal A breast cancer patients. In this study, we aimed to determine the molecular changes PI3K/AKT/PTEN signaling pathway during tamoxifen-resistance development using gradually increased doses tamoxifen one model, while fixing treatment dose at 35 μM for several times second model. An upregulation AKT/PI3K genes was noticed 30 concentration cells treated with gradual increase doses. significant AKT1 seen...
Tamoxifen is commonly used in the treatment of hormonal-positive breast cancer. However, 30%-40% tumors treated with tamoxifen develop resistance; therefore, an important step to overcome this resistance understand underlying molecular and metabolic mechanisms. In present work, we profiling determine potential biomarkers resistance, gene expression levels enzymes these metabolites then correlated survival patients receiving tamoxifen.
Lactate dehydrogenase (LDH) is a key enzyme in the last step of glycolysis, playing role pyruvate-to-lactate reaction. It associated with prognosis and metastasis many cancers, including breast cancer. In this study, we investigated changes LDH gene expression lactate concentrations culture media during tamoxifen resistance development MCF-7 cell line, examined LDHB promoter methylation levels. An upregulation 2.9 times was observed around IC50 concentration treated cells, while fluctuation...
Tobacco smoking is a preventable main cause of fatal diseases. Accurate measurements the effects it has on neurotransmitters are essential in developing new strategies for cessation. Moreover, neurotransmitter levels can aid drugs that counteract smoking. The aim this study to develop and validate fast, simultaneous sensitive method measuring rat brain after exposure tobacco cigarettes. selected include dopamine, GABA, serotonin, glutamine glutamate. based high-performance liquid...
Glaucoma is considered the second most common cause of blindness in patients above age 50. Lack adherence to glaucoma medications frequently results undesirable complications, specifically and disability.
Cigarette smoke withdrawal can cause anxiety-like behavior and modulate neurotransmitter-related proteins in the brain. We examined effects of cigarette with without aspirin treatment on concentrations neurotransmitters, including dopamine, serotonin, glutamate, glutamine, GABA amygdala hippocampus. Sprague-Dawley rats were randomly assigned to four different groups: (1) control group exposed only standard room air, (2) treated saline vehicle, (3) (30 mg/kg), (4) mg/kg). exposure was...
Paracetamol (acetaminophen) is one of the most commonly used antipyretic and analgesic drugs worldwide.It drug choice for patients with bronchial asthma, hemophilia, salicylate hypersensitivity, peptic ulcer, pregnant or breastfeeding women who cannot be treated nonsteroidal anti-inflammatory drugs.It marketed manufactured by many pharmaceutical companies, which necessitates requirement quality control investigation.A post-market evaluation was conducted on five commercial paracetamol...
Abstract Electronic nicotine delivery systems (ENDs) are gaining popularity in Jordan as alternatives to tobacco cigarettes with an estimation of 10% smokers switching ENDs. Since is toxic and highly addictive substance, it important develop validate easy rapid analytical method accurately measure level e-liquids. A simple high performance liquid chromatography–photodiode array detection (HPLC–PDA) was developed validated for determination the actual content 11 most popular e-liquids brands...
Breast cancer is one of the most prevalent cancers worldwide usually treated with Tamoxifen. Tamoxifen resistance development challenging issue in an initially responsive breast tumor, and mechanisms are still under investigation. The objective this study to develop validate a selective, sensitive, simultaneous high performance liquid chromatography–tandem mass spectrometry method explore changes substrates metabolites supernatant media developed MCF-7 cells. We focus on determination...
Abstract Purpose Development and validation of a selective analytical method to accurately precisely quantify nicotine cotinine levels in rat's plasma after exposure tobacco cigarettes water-pipe. Methods An easy HPLC-Photodiode-Array Detection (PDA) was developed validated for simultaneous determination 15 rats (10 products 5 control rats). Nicotine were extracted one step from using acetonitrile concentrated lowest volume nitrogen stream. Results The offered rapid analysis time 14 min with...
Abstract Early stage pharmacological studies rely on in vitro methodologies for screening and testing compounds. Conventional assays based endpoint measurements provide limited information because the lack temporal resolution may not determine effect at its maximum. We developed an on-line, automated system near real-time monitoring of extracellular content from five parallel suspension cultures, combining cell density with a high-resolution separations every 12 minutes 4 days. Selector...
Breast cancer is the second leading cause of death in women after lung cancer. The first-line treatment metastatic breast premenopausal relies on tamoxifen. development tamoxifen resistance not fully understood. In this study, capillary electrophoresis with capacitively coupled contactless conductivity detector was developed to monitor changes lactate and pyruvate levels supernatant media three models MCF-7 tamoxifen-resistant cells correlate these metabolites dehydrogenase genes expression...