A floc-forming, Gram-stain-negative, petroleum hydrocarbon-degrading bacterial strain, designated Buc(T), was isolated from a hydrocarbon-contaminated site in Hungary. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Buc(T) formed distinct phyletic lineage within the genus Zoogloea. Its closest relative found to be Zoogloea caeni EMB43(T) (97.2% sequence similarity) followed by oryzae A-7(T) (95.9%), ramigera ATCC 19544(T) (95.5%) and resiniphila DhA-35(T) (95.4%)....

Alkaliphilic strains characterized by optimal growth at pH 9.0 and 5 % (w/v) NaCl designated K1-25(T) H3-93 were isolated from extremely shallow soda ponds located in Hungary. Cells of both Gram-stain-positive, non-motile, straight rods formed central, ellipsoidal endospores with swollen sporangia. The isolates aerobic, catalase-positive, oxidase-negative contained a peptidoglycan type A1γ based on meso-diaminopimelic acid. In strains, menaquinone-7 (MK-7) was the predominant isoprenoid...

A strain designated PYM3-14T was isolated from the drinking water network of Budapest (Hungary) and studied by polyphasic taxonomic methods. The straight-rod-shaped cells stained Gram-negative, were aerobic non-motile. Phylogenetic analysis 16S rRNA gene sequence revealed a clear affiliation with members family Xanthomonadaceae within class Gammaproteobacteria. showed closest similarities to Arenimonas daechungensis CH15-1T (96.2 %), oryziterrae YC6267T (95.2 %) Lysobacter brunescens UASM DT...

A Gram-negative-staining, short-rod-shaped, floc-forming bacterium, designated strain RB3-7(T), was isolated from a laboratory-scale activated sludge system treating coke plant effluent. Comparative analysis of the 16S rRNA gene sequence demonstrated that novel isolate distantly related (≤ 95.8 % similarity) to Ottowia thiooxydans K11(T) within family Comamonadaceae. Strain RB3-7(T) catalase- and oxidase-positive non-motile. The predominant fatty acids were C₁₆:₀, cyclo C₁₇:₀, C₁₈:₁ω7c...

A Gram-stain-negative, rod-shaped bacterium (strain K-39 T ) was isolated from the thermophilic phase of composting process for oyster mushroom substrate preparation. The strain grew at 40–80 °C (optimum, 65–75 °C), pH 5–9 7), in media containing up to 1.5 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that formed a distinct lineage within genus Thermus . Its closest cultivated relative islandicus PRI 3838 (96.8 similarity). DNA G+C content 71.3 mol%. new could...

A novel bacterium, PB3-7B(T), was isolated on phenol-supplemented inorganic growth medium from a laboratory-scale wastewater purification system that treated coke plant effluent. 16S rRNA gene sequence analysis revealed strain PB3-7B(T) belonged to the family Alcaligenaceae and showed highest pairwise similarity Parapusillimonas granuli Ch07(T) (97.5%), Candidimonas bauzanensis BZ59(T) (97.3%) Pusillimonas noertemannii BN9(T) (97.2%). Strain rod-shaped, motile oxidase- catalase-positive. The...

A novel alphaproteobacterium, strain RAM11T, belonging to the family Rhizobiaceae was isolated from pool water of a thermal bath in Budapest, Hungary. Based on 16S rRNA gene sequence RAM11T shows highest similarity values Ensifer adhaerens Casida (97.44 %), (syn. Sinorhizobium) americanus CFNEI 156T (96.87 %) and Rhizobium azooxidifex Po 20/26T (96.76 %). The new bacterium is strictly aerobic, its optimum growth occurs at 20-37 °C, between pH 7 9 without NaCl. It motile due single polar...

Because of the selectivity commonly used media it is very difficult to cultivate bacteria inhabiting ultrapure waters under laboratory conditions. In this study 5 new (synthetic and complex) were developed reveal bacterial community water originated from purification system a Hungarian power plant which was studied already with using traditional media. Composition tends reproduce nutrient deficient conditions investigated water, therefore highly oligotrophic. Altogether 122 strains isolated...

Thermal baths are unique aquatic environments combining a wide variety of natural and anthropogenic ecological factors, which also appear in their microbiological state. There is limited information on the microbiology thermal complexity, tracking community shifts from wells to pools. In present study, microbial well pool waters Gellért bath was studied detail by cultivation-based techniques. To isolate bacteria, 10% R2A minimal synthetic media (with "bath water") with agar-agar gellan gum...

Many industries use ultrapure water (UPW) for their operation. In spite of purification efforts, microbial contaminations are the major cause manufacturing problems in these systems. our experiments, system a power plant was investigated: microbiologically critical points were located by checking number and diversity bacteria to determine optimum operational unit chemical intervention: most contaminated site mixed-bed ion-exchange resin containing unit. Biocides tested against previously...

Chlorine has a strong antibacterial property and is the disinfectant most frequently used in swimming pools. Therefore, microbiota community oral cavity of those who practice water sports assumed to be special due their regular immersion water. Adverse changes composition may have serious health consequences. We aimed compare microbiome between polo players non-athletes. hypothesized that differed non-athletes.Altogether, 124 (62 males 62 females, aged 9 20 years) 16 non-athlete youths...

Eudiaptomus gracilis is the most abundant member of zooplankton, plays a key role in food web Lake Balaton (Hungary). In present study composition bacterial communities this copepod was investigated based on cultivation and molecular cloning. The cultivated strains from gut homogenate samples belonged to four different clades: Firmicutes, Actinobacteria, Bacteriodetes Proteobacteria. Clone library showed high species diversity, Proteobacteria, representatives Deinococcus-Thermus lineage...

A novel Gram-stain-positive bacterial strain, designated as K13T, was isolated from compost and characterized using a polyphasic approach to determine its taxonomic position. On the basis of 16S rRNA gene sequence analysis, strain showed highest similarity (93.8 %) Paenibacillus nanensis MX2-3T. Cells K13T were aerobic, motile rods. The major fatty acids anteiso C15 : 0 (34.4 %), iso C16 (17.3 (10.0 %). menaquinone MK-7, polar lipid profile included diphosphatidylglycerol,...

A new betaproteobacterium, CGII-59m2T, was isolated from an activated sludge bioreactor which treated landfill leachate. The 16S rRNA gene sequence analysis revealed that strain CGII-59m2T belonged to the family Alcaligenaceae and shared highest pairwise similarity values with Parapusillimonas granuli LMG 24012T (97.7 %), various species of genus Bordetella (97.3-97.0 %) Candidimonas nitroreducens 24812T (97.0 %). Cells were rod-shaped, non-motile, oxidase- catalase-positive. predominant...

A Gram-negative bacterium, designated P2(T), was isolated from the biofilm developed on inner surface of an ultrapure cooling water system in a Hungarian power plant and characterized by polyphasic approach. Phylogenetic analysis based 16S rRNA gene sequences revealed that strain P2(T) affiliated with family 'Flexibacteraceae' phylum Bacteroidetes. Its closest relative Flectobacillus lacus CL-GP79(T) (88.7 % sequence similarity) followed Arcicella rosea TW5(T) (86.5 %), aquatica NO-502(T)...

Three Gram-positive, rod-shaped bacterial strains were isolated from the drinking water supply system of Hungarian capital, Budapest. Phylogenetic analysis on basis 16S rRNA gene sequence comparison revealed that isolates represented a distinct cluster within clade genus Nocardioides and most closely related to pyridinolyticus OS4(T), aquiterrae GW-9(T), sediminis MSL-01(T) N. hankookensis DS-30(T). The peptidoglycan based LL-2,6-diaminopimelic acid, major menaquinone MK-8(H₄), cellular...

Ultrapure waters (UPWs) containing low levels of organic and inorganic compounds provide extreme environment. On contrary to that microbes occur in such form biofilms on surfaces, thus may induce corrosion processes many industrial applications. In our study, refined saltless water (UPW) produced for the boiler a Hungarian power plant was examined before after storage (sampling inlet [TKE] outlet [TKU] tank) with cultivation culture independent methods. Our results showed increased CFU...

A Gram-stain-positive bacterium, designated TSL3T, was isolated from faeces of a porcupine, Hystrix indica, the Budapest Zoo and Botanical Garden, Hungary. On basis 16S rRNA gene sequence analysis, strain is phylogenetically related to family Micrococcaceae. The highest similarity found with Micrococcus terreus V3M1T (96.50 %) followed by Arthrobacter humicola KV-653T (96.43 %). Cells TSL3T were aerobic, non-motile coccoid-shaped. main fatty acids anteiso-C15 : 0 (54.4 %), iso-C16 (18.2 iso...

A novel bacterium, CAI-18bT, was isolated from a bioreactor that treated landfill leachate using an oligotrophic growth medium. Phylogenetic analysis based on the 16S rRNA gene sequence revealed strain CAI-18bT is member of genus Rufibacter, showing 97.1 % pairwise similarity to Rufibacter roseus H359T, 96.4 tibetensis 1351T, glacialis MDT1-10-3T and 96.0 immobilis MCC P1T. Strain rod-shaped, motile, oxidase- catalase-positive. The predominant fatty acids were iso-C15 : 0 (24.1 %) iso-C17 1...

A new aerobic betaproteobacterium, strain SA-152T, was isolated from the water of a crater lake. 16S rRNA gene sequence analysis revealed that SA-152T belonged to family Oxalobacteraceae (order Burkholderiales ) and phylogenetically related Solimicrobium silvestre S20-91T with 97.09 % Herminiimonas arsenicoxydans ULPAs1T 96.00 pairwise similarity. Cells were rod-shaped, non-motile, oxidase-negative catalase-positive. Its fatty acid profile dominated by two acids, C16 : 1 ω7c 0, major...

Abstract Objectives. Adverse changes in the constitution of oral cavity microbiota may have serious health consequences. We hypothesized that community water polo players differ from others. Our aims were to determine composition microbiome elite and compare their non-athletes. Materials Methods Elite (n: 29) non-athletes 16) aged between 16–20 studied. The was determined a saliva sample, DNA isolated by QIAmp Blood Mini Kit. 16S rRNA gene amplicon sequencing method used analyse community....