Here, a dual-wavelength ratiometric electrochemiluminescence (ECL) approach is reported based on resonance energy transfer (RET) from graphite-like carbon nitride nanosheet (g-C3N4 NS) to Ru(bpy)32+ for sensitive detection of microRNA (miRNA). In this approach, Au nanoparticles (Au NPs) functionalized g-C3N4 NS nanohybrid (Au-g-C3N4 NH) coated glassy electrode (GCE) could exhibit strong and stable ECL emissions with emission peak centered at 460 nm. The such wavelength matched well the...

Multifunctional theranostic nanoplatforms (NPs) in response to environment stimulations for on-demand drug release are highly desirable. Herein, the near-infrared (NIR)-absorbing dye, indocyanine green (ICG), and antitumor drug, doxorubicin (DOX), were efficiently coencapsulated into thermosensitive liposomes based on natural phase-change material. Folate conjugated gadolinium (Gd) chelate-modified liposome shells enhance active targeting magnetic resonance performance of NPs while...

Selenocysteine (Sec) is the 21st naturally occurring amino acid and has emerged as an important sensing target in recent years. However, fluorescent detection of Sec living systems challenging. To date, very few probes have been reported most them respond fluorescence to visible region. In this paper, a promising near-infrared probe for was developed. This works aqueous solution over wide pH range under mild conditions can be used rapid, highly selective sensitive with significant turn-on...

Quantification of trace serum circulate microRNAs is extremely important in clinical diagnosis but remains a great challenge. Herein we developed an ultrasensitive platform for microRNA 141 (miR-141) detection based on silver coated gold nanorods (Au@Ag NRs) etching process accompanied by surface plasmon resonance (SPR) shift. Both SPR absorption and scattering responses were monitored. Combined amplification cascades catalyzed hairpin assembly (CHA) hybridization chain reaction (HCR) with...

Abstract The success of phototheranostics is hampered by some intrinsic defects, such as limited light penetration depth, heat resistance tumor cells to photothermal therapy (PTT) induced shock protein (HSP) and stress against photodynamic (PDT) caused hypoxia microenvironment tumor. Herein, a second near infrared (NIR‐II) excitation phototheranostic nanomedicine has been fabricated integrating the semiconducting polymer, azo compound, HSP inhibitor into thermosensitive liposome, followed...

The development of effective and safe tumor nanotheranostics remains a research imperative. Herein, microenvironment (TME)-responsive Fe(III)-porphyrin (TCPP) coordination nanoparticles (FT@HA NPs) were prepared using simple one-pot method followed by modification with hyaluronic acid (HA). FT@HA NPs specifically accumulated in CD44 receptor-overexpressed tissues through the targeting property HA upon endocytosis cells. After cell internalization, intracellular acidic microenvironments high...

DNA/RNA strand displacement is one of the most fundamental reactions in DNA and RNA circuits nanomachines. In this work, we reported an exploration dynamic process toehold-mediated via core–satellite plasmon rulers at single-molecule level. Applying with unlimited lifetime, single-strand triggered by invader that resulted stepwise leaving satellite from core was continuously monitored changes scattering signal for hours. The kinetics vitro three different toehold lengths have been...

Phototherapy, including photothermal therapy (PTT) and photodynamic (PDT), has been considered as a noninvasive option for cancer therapy. However, insufficient penetration depth, tumor hypoxia, single treatment method severely limit the effectiveness of treatment. Methods: In this study, multifunctional theranostic nanoplatform fabricated based on Au/Ag-MnO2 hollow nanospheres (AAM HNSs). The Au/Ag alloy HNSs were first synthesized by galvanic replacement reaction then MnO2 nanoparticles...

Abstract The phototheranostics in the second near‐infrared window (NIR‐II) have proven to be promising for precise cancer theranostics. However, non‐responsive and “always on” imaging mode lacks selectivity, leading poor diagnosis specificity. Herein, a tumor microenvironment (TME) activated NIR‐II phototheranostic nanoplatform (Ag 2 S‐Fe(III)‐DBZ Pdots, AFD NPs) is designed based on principle of Förster resonance energy transfer (FRET). NPs are fabricated through self‐assembly Ag S QDs...

Gold nanodendrites (Au NDs) exhibit extremely strong electromagnetic field located around multiple tip branches due to a plasmon coupling effect. In this work, novel LSPR-enhanced ECL emission from CdTe nanocrystals (NCs) by Au NDs for the detection of nucleic acid is reported. This system composed thin film NCs on glassy carbon electrode (GCE) as anodic emitter and enhancer. DNA tetrahedron embedded with stem-loop hairpin structure one edge was applied switch regulate distance between NDs....

Photodynamic therapy (PDT) is becoming a promising therapeutic regimen but limited by the hypoxic microenvironment in solid tumors and undesirable post-treatment phototoxicity side effects on normal tissues. To overcome these restrictions enhance antitumor effect, near-infrared (NIR) light-activated, cancer cell-specific, hypoxia prodrug-loaded chlorin e6 liposomes were developed for tumor selective combination guided multimodal imaging. The photothermal agent indocyanine green (ICG)...

Theoretical and experimental studies of plasmon enhanced electrochemiluminescence CdS QDs by gold nanoparticle monomers dimers.

We report a new strategy for detection of the methylation level and position in Hsp53 tumor suppressor gene based on electrochemiluminescence signal amplification combined with conformation-switched hairpin DNA probe improving selectivity.

Subcellular organelle mitochondria are becoming a key player and driver of cancer. Mitochondrial targeting phototheranostics has attracted increasing attention for precise cancer therapy. However, those phototheranostic systems still face great challenges, including complex multiple components, light scattering, insufficient therapeutic efficacy. Herein, molecular fluorophore IR-TPP-1100 was tactfully designed by engineering mitochondria-targeted fluorescence imaging-guided phototherapy in...

Abstract The precise detection of multiple microRNAs (miRNAs) is great significance to the classification various cell subtypes. However, miRNA hindered by signal leakage, false‐positive signals and low expression miRNAs. Herein, a DNA nanodevice in second near infrared region (NIR‐II, 1000‐1700 nm) (DND‐II) developed for dual miRNAs bioimaging based on “AND” logic‐gate amplification strategy. spatiotemporally controlled logic‐gate‐based DND‐II fabricated thermosensitive liposomes integrated...

An injectable hydrogel sustained drug release system could be a promising technique for in situ treatment. Herein, an was prepared photothermal-chemo therapy of cancer based on the thermosensitive liposomal (Lip-Gel). The Lip-Gel fabricated by encapsulation NIR-II photothermal agent (DPP-BTz) and chemotherapy drugs (GEM) liposomes then combined with precursor solution. used as flowing solution at room temperature transferred into cross-linked gel structure physiological temperature. After...

Innovative techniques to measure microRNA (miRNA) in vivo could greatly improve the fundamental understanding of complex cellular processes. Herein, we report a novel method for real-time, quantitative miRNA detection inside living cells based on core–satellite plasmon rulers (PRs). This approach allows statistical analysis single hybridization event caused by target miRNA. We investigated hundreds satellite leaving events and found that distribution time range one strand displacement is...