A5073128953- Multiple Sclerosis Research Studies
- Systemic Lupus Erythematosus Research
- Spectroscopy Techniques in Biomedical and Chemical Research
- Ultrasound and Hyperthermia Applications
- Advanced Neuroimaging Techniques and Applications
- Peripheral Neuropathies and Disorders
- Advancements in Transdermal Drug Delivery
- Photoacoustic and Ultrasonic Imaging
- Dementia and Cognitive Impairment Research
- RNA regulation and disease
- Lipid Membrane Structure and Behavior
- RNA Research and Splicing
- Advanced MRI Techniques and Applications
- Digital Imaging for Blood Diseases
- Alzheimer's disease research and treatments
- Endoplasmic Reticulum Stress and Disease
- Cell death mechanisms and regulation
- Digestive system and related health
- Systemic Sclerosis and Related Diseases
- thermodynamics and calorimetric analyses
- Ultrasound Imaging and Elastography
- Neurogenesis and neuroplasticity mechanisms
- Molecular Biology Techniques and Applications
- Neurological Disease Mechanisms and Treatments
- RNA Interference and Gene Delivery
Amsterdam University of Applied Sciences
2018
University Medical Center
2018
University Hospital and Clinics
2018
Amsterdam UMC Location Vrije Universiteit Amsterdam
2013-2017
Center for Neurosciences
2015-2017
Amsterdam Neuroscience
2015-2016
Vrije Universiteit Amsterdam
2013-2015
The relevance of cortical grey matter pathology in multiple sclerosis has become increasingly recognized over the past decade.Unfortunately, a large part lesions remain undetected on magnetic resonance imaging using standard field strength.In vivo studies have shown improved detection by higher strengths up to 7 T.So far, systematic histopathological verification ultra-high pulse sequences been lacking.The aim this study was determine sensitivity T versus 3 for directly comparing them...
In multiple sclerosis, neuropathological studies have shown widespread changes in the cerebral cortex. vivo imaging is critical, because histopathological substrate of most measurements unknown.Using a novel magnetic resonance analysis technique, based on ratio T1- and T2-weighted signal intensities, we studied cortex large cohort patients early stages sclerosis. A total 168 with clinically isolated syndrome or relapsing-remitting sclerosis (Expanded Disability Status Scale: median = 1,...
Background: Cortical atrophy, assessed with magnetic resonance imaging (MRI), is an important outcome measure in multiple sclerosis (MS) studies. However, the underlying histopathology of cortical volume measures unknown. Objective: We investigated histopathological substrate MRI-measured MS using combined post-mortem and histopathology. Methods: brain donors underwent whole-brain in-situ MRI imaging. After MRI, tissue blocks were systematically sampled from superior inferior frontal gyrus,...
Cellular injury and death are ubiquitous features of disease, yet tools to detect them limited insensitive subtle pathological changes. Acridine orange (AO), a nucleic acid dye with unique spectral properties, enables real-time measurement RNA DNA as proxies for cell viability during exposure various noxious stimuli. This tool illuminates signatures modes death, such cells undergoing apoptosis versus necrosis/necroptosis. new approach also shows that cellular decreases necrotic, necroptotic,...
Multiple sclerosis (MS) is a demyelinating and neurodegenerative disease of the CNS. lesions include significant demyelination gray matter, which thought to be major contributor both physical cognitive impairment. Subpial (Type III) are most common demyelinated cortical lesions. We investigated features subpial in cerebral cortex samples from 11 patients with MS 6 nondemented non-MS controls. There were no differences neuron axon density between normally myelinated normal-appearing matter...
Using diffusion tensor imaging (DTI), it was previously found that demyelinated gray matter (GM) lesions have increased fractional anisotropy (FA) when compared to normal-appearing (NAGM) in multiple sclerosis (MS). The biological substrate underlying this FA change is so far unclear; both neurodegenerative changes and microglial activation been proposed as causal contributors.To test the hypothesis microglia responsible for cortical GM lesions.We investigated post-mortem DTI hemispheric,...
Grey matter (GM) atrophy is a prominent aspect of multiple sclerosis pathology and an important outcome in studies.GM measurement requires accurate GM segmentation.Several methods are used vivo for measuring volumes MS, but assessing their validity remains challenging.In this postmortem study, we evaluated the correlation between MRI cortical volume or thickness measured on histological sections.Sixteen MS brains were scanned situ using 3DT1-weighted these images to measure regional...
<h3>BACKGROUND AND PURPOSE:</h3> At 7T MR imaging, T2*-weighted gradient echo has been shown to provide high-resolution anatomic images of gray matter lesions. However, few studies have verified T2*WI lesions histopathologically or compared them with more standard techniques at ultra-high-field strength. This study aimed determine the sensitivity T2WI and sequences for detecting cortical GM in MS. <h3>MATERIALS METHODS:</h3> 7T, 2D multiecho spin-echo 3D gradient-echo were acquired from 27...
Multiple sclerosis white matter exhibits widespread biochemical lipid pathology of myelin measured by label-free spectral CARS microscopy.
The pathology of multiple sclerosis (MS) involves both the gray and white matter regions brain spinal cord. It is characterized by various combinations demyelination, inflammatory infiltration, axonal degeneration, later gliosis in chronic lesions. While acute plaques are well easily identified, evidence indicates that CNS MS patients may be globally altered, with subtle abnormalities found grossly normal appearing (NAWM) diffusely abnormal (DAWM) where histochemical stains advanced magnetic...
The pathology of multiple sclerosis involves the gray and white matter regions brain spinal cord often characterized by various combinations demyelination, inflammatory infiltration, axonal degeneration, later gliosis in chronic lesions. While acute lesions are well easily identified, evidence indicates that CNS MS patients may be globally altered, with subtle abnormalities found grossly normal appearing (NAWM) histochemical stains magnetic resonance imaging only indicating a general...
The classical distribution pattern for neurofibrillary tangles (NFT) is not seen in all Alzheimer’s disease (AD) patients (Murray, 2011). Of patients, 6-14% presents with non-amnestic symptoms such as language and visuospatial dysfunction (Dubois, 2014). There an association between the severity of NFT pathology parietal cortex (Tang Wai, 2004). We hypothesize that next to occurrence phosphorylated tau, amyloid beta microglia differ cortical amnestic (AAD) AD (NAAD) patients. AAD cases...