A5018041238- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Phytoplasmas and Hemiptera pathogens
- Plant Pathogens and Fungal Diseases
- Cocoa and Sweet Potato Agronomy
- Insect symbiosis and bacterial influences
- Legume Nitrogen Fixing Symbiosis
- Agriculture, Soil, Plant Science
- Listeria monocytogenes in Food Safety
- Food Safety and Hygiene
- Salmonella and Campylobacter epidemiology
- Plant Virus Research Studies
- Identification and Quantification in Food
- Food Quality and Safety Studies
- Studies on Chitinases and Chitosanases
- Banana Cultivation and Research
- Phytochemical compounds biological activities
- Plant Disease Management Techniques
- Nutrition, Health and Food Behavior
- Peptidase Inhibition and Analysis
- Microbial Metabolism and Applications
- Microbial Inactivation Methods
- Infections and bacterial resistance
- Amino Acid Enzymes and Metabolism
- Microbial infections and disease research
University of California, Davis
2015-2024
Plant (United States)
2020-2021
University of California, Riverside
2020
Kangwon National University
2010-2012
Abstract ‘ Candidatus Liberibacter’ species are insect‐transmitted, phloem‐limited α‐Proteobacteria in the order of Rhizobiales . The citrus industry is facing significant challenges due to huanglongbing, associated with infection from Liberibacter asiaticus’ (Las). In gain greater insight into Ca. biology and genetic diversity, we have performed genome sequencing comparative analyses diverse Ca species, including those that can infect citrus. Our phylogenetic analysis differentiates...
Clavibacter michiganensis subsp. is a gram-positive bacterial pathogen that proliferates in the xylem vessels of tomato, causing canker disease. In this study, we sequenced and assembled genomes 11 C. strains isolated from infected tomato fields California as well five colonize endophytically but are not pathogenic host. The analysis supported monophyletic nature revealed genetic diversity among strains, consistent with multiple introduction events. Two endophytes clustered phylogenetically...
The citrus industry is facing an unprecedented crisis due to Huanglongbing (HLB, aka greening disease), a bacterial disease associated with the pathogen Candidatus Liberibacter asiaticus (CLas) that affects all commercial varieties. Transmitted by Asian psyllid (ACP), CLas colonizes phloem, leading reduced yield and fruit quality, eventually tree decline death. Since adequate curative measures are not available, key step in HLB management restrict spread of identifying infected trees...
Gram-positive bacteria are prominent members of plant-associated microbial communities. Although many hypothesized to be beneficial, some causative agents economically important diseases crop plants. Because the features fundamentally different relative those Gram-negative bacteria, evolution and ecology as well mechanisms used colonize infect plants also differ. Here, we discuss recent advances in our understanding Gram-positive, provide a framework for future research directions on these...
Although much is known about the responses of model plants to microbial features, we still lack an understanding extent variation in immune perception across members a plant family. In this work, analyzed Citrus and wild relatives, surveying 86 Rutaceae genotypes with differing leaf morphologies disease resistances. We found that features vary both within between members. Species 2 subtribes, Balsamocitrinae Clauseninae, can recognize flagellin (flg22), cold shock protein (csp22), chitin,...
"Candidatus Liberibacter spp." are insect-vectored, fastidious, and vascular-limited phytopathogens. They the presumptive causal agents of potato zebra chip, tomato vein clearing, devastating citrus greening disease worldwide. There is an urgent need to develop new strategies control them. In this study, we characterized a dual-specificity serine/tyrosine phosphatase (STP) that well conserved among thirty-three geographically diverse "
The Japanese Erwinia strain Ejp617 is a plant pathogen that causes bacterial shoot blight of pear in Japan. Here, we report the complete genome sequence isolated from Nashi pears Japan to provide further valuable insight among related species.
Clavibacter michiganensis is the causal agent of bacterial canker tomato, which causes significant economic losses because lack resistant tomato varieties. Chemical control with streptomycin or cupric bactericides last defensive line in disease management. Streptomycin an aminoglycoside antibiotic that inhibits protein synthesis and targets 30S ribosomal RpsL. has been used to multiple plant diseases. However, identification characterization resistance C. have remained unexplored. In this...
Huanglongbing (HLB) is the most devastating and widespread citrus disease. All commercial varieties are susceptible to HLB-associated bacterium,
Abstract Background Spiroplasma citri comprises a bacterial complex that cause diseases in citrus, horseradish, carrot, sesame, and also infects wide array of ornamental weed species. S. is transmitted persistent propagative manner by the beet leafhopper, Neoaliturus tenellus North America Circulifer haematoceps Mediterranean region. Leafhopper transmission pathogen’s host range serve as drivers genetic diversity. This diversity was examined silico comparing genome sequences seven strains...
Clavibacter michiganensis is a Gram-positive bacterial pathogen that proliferates in the xylem vessels of tomato, causing wilt and canker symptoms. Accurate detection crucial step confirming outbreaks developing management strategies. A major problem with existing methods are false-positive -negative results. Here, we report use comparative genomics 37 diverse strains, including 21 strains sequenced this study, to identify specific sequences C. targets. Genome-wide phylogenic analyses...
ABSTRACT Pseudomonas syringae pv. tomato race 1 strains have evolved to overcome genetic resistance in tomato. Here, we present the draft genome sequences of two P. strains, A9 and 407, isolated from diseased plants California.
Climate change is predicted to increase the prevalence of vector-borne disease due expansion insect populations. 'Candidatus Liberibacter solanacearum' a phloem-limited pathogen associated with multiple economically important diseases in solanaceous crops. Little known about strategies and pathogenicity factors 'Ca. L. uses colonize its vector host. We determined effector repertoire by predicting proteins secreted general secretory pathway across four different haplotypes, investigated...
The potential antiviral effects of the culture filtrates (CF) from Serratia marcescens strain Gsm01 against yellow Cucumber mosaic virus (CMV-Y) were investigated. filtrate S. applied on Chenopodium amaranticolor showed high inhibitory activity, likewise no necrosis appeared when tobacco plants 2 days before CMV-Y inoculation. When challenge inoculated with for eighteen days, disease incidence in did not exceed 59%, whereas 100% control severely infected. results double antibody...
Japanese Erwinia pyrifoliae strains cause bacterial shoot blight of pear (BSBP) in Japan. The genetics remains largely unknown relative to the abundant genomic information available for other strains. We compared genome and Korean E. along with those amylovora tasmaniensis. Comparisons strain revealed numerous gene insertions/deletions, rearrangements, inversions central regions chromosomes. Approximately 80% (2843) coding DNA sequences (CDSs) are shared by these two genomes which represent...
A total of 142 samples comprising vegetables, soil, and water collected from different agricultural farms (five provinces) were analyzed for aerobic bacteria (aerobic plate count [APC]), Bacillus cereus, Escherichia coli, Listeria monocytogenes, Salmonella spp., Staphylococcus aureus. The average APC in all the ranged <TEX>$4.72{\times}10^5$</TEX> to <TEX>$8.62{\times}10^8\;CFU/g$</TEX> (mL). prevalence B. E. L. S. aureus was 17.60%, 2.11 %, 1.4%, 0%, % respectively, their counts averaged...
A rapid high-throughput, specific and sensitive PCR-based assay coupled with DNA hybridization technique for the detection of deep bark canker (DBC) pathogenBrenneria rubrifaciens directly from mixed bacterial cells and in planta was developed. These result shows that genetic markers have a powerful potential to detect B. crude samples at field conditions. Key words: Brenneria rubrifaciens, canker, PCR, rubrifacine.
We designed a sensitive and specific PCR-based method with enterobacterial repetitive intergenic consensus (ERIC) primer to detect Erwinia pyrifoliae, which cause shoot blight in Asian pear, from mixed culture infected plant materials. The primers specifically detected only E. pyrifoliae showed no cross-reactivity other bacterial phytopathogens.
Abstract Huanglongbing (HLB) is the most devastating and widespread citrus disease. All commercial varieties are susceptible to HLB-associated bacterium, Candidatus Liberibacter asiaticus ( C Las), which resides in phloem. The phloem part of plant vascular system involved sugar transport. To investigate response Las, we enriched for proteins surrounding an HLB sweet orange variety, Washington navel Citrus sinensis (L) Osbeck). Quantitative proteomics revealed global changes proteome after...