A5057464314- Cancer-related Molecular Pathways
- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- RNA modifications and cancer
- RNA Interference and Gene Delivery
- Cancer-related gene regulation
- Cancer Cells and Metastasis
- Ovarian cancer diagnosis and treatment
- DNA Repair Mechanisms
- FOXO transcription factor regulation
- Genomics and Chromatin Dynamics
- Ubiquitin and proteasome pathways
- Mosquito-borne diseases and control
- 14-3-3 protein interactions
- Sirtuins and Resveratrol in Medicine
- Genetics and Neurodevelopmental Disorders
- RNA Research and Splicing
- Viral Infections and Vectors
- PARP inhibition in cancer therapy
- Virology and Viral Diseases
- Cancer-related molecular mechanisms research
- Cancer Mechanisms and Therapy
- Advanced biosensing and bioanalysis techniques
- Microtubule and mitosis dynamics
- Hedgehog Signaling Pathway Studies
Midwestern University
2023-2025
Yale University
2017-2021
University of New Haven
2020
City of Hope
2012-2018
Beckman Research Institute
2012-2018
Fred Hutch Cancer Center
1984
Breast cancer is the leading cause of cancer-related deaths among women in United States, and survival rates are lower for patients with metastases and/or triple-negative breast (TNBC; ER, PR, Her2 negative). Understanding mechanisms metastasis therefore crucial to identify new therapeutic targets develop novel treatments improve patient outcomes. A potential target TWIST1 transcription factor, which often overexpressed aggressive cancers a master regulator cellular migration through...
Abstract Epithelial ovarian cancer (EOC) is the most deadly gynaecologic malignancy due to late onset of symptoms and propensity towards drug resistance. Epithelial-mesenchymal transition (EMT) has been linked development chemoresistance in other cancers, yet little known regarding its role EOC. In this study, we sought determine transcription factor TWIST1, a master regulator EMT, on cisplatin resistance an EOC model. We created two Ovcar8-derived cell lines that differed only their TWIST1...
Advances in our understanding of the molecular mechanisms homologous recombination have come from a variety experimental approaches. Genetic analyses mutants defective identified genes whose functions are required for and led to concept alternative pathways recombination. Biochemical wild-type mutant cells enzymatic activities associated with some these therefore necessary Studies on transfer genetic information material parental DNA molecules recombinant shown cases be breakage reunion...
A growing body of evidence has demonstrated the promising anti-tumor effects resveratrol in ovarian cancer cells, including its inhibitory on STAT3 activation. Nonetheless, low bioavailability reduced attractiveness as a potential anti-cancer treatment. In contrast, pterostilbene, stilbenoid and analog, superior bioavailability, while possessing significant antitumor activity multiple solid tumors. this study, therapeutic pterostilbene was evaluated cells. Pterostilbene reduces cell...
Abstract Epithelial–mesenchymal transition (EMT) is a critical process involved in cancer metastasis and chemoresistance. Twist1 key EMT-inducing transcription factor, which upregulated multiple types of cancers has been shown to promote tumor cell invasiveness support progression. Conversely, p53 suppressor gene that frequently mutated cancers. This study demonstrates the ability wild-type (WT) degradation protein. By forming complex with E3 ligase Pirh2, WT promotes ubiquitination...
Background/Objectives: Oral squamous cell carcinoma (OSCC) is the most common form of head and neck cancer accounts for over 50,000 new cases annually in United States. The survival rates are markedly different localized OSCC versus metastatic disease, which five-year rate only 39%. Depending on its pathology stage at diagnosis, treatment may involve surgery, radiation, targeted therapy, or conventional chemotherapy. However, there an unmet need reliable biomarkers to predict response link...
Endometrial cancer is the most common gynecologic in United States and its incidence mortality has been rising over past decade. Few treatment options are available for patients with advanced recurring endometrial cancers. Novel therapies, which frequently toxic, difficult to establish this patient population tends be older plagued by comorbidities such as diabetes mellitus hypertension. Therefore, novel, non-toxic therapies urgently needed. Megestrol acetate a used drug patients. However,...
Epithelial ovarian cancer (EOC) remains the most lethal gynecologic malignancy, largely due to metastasis and drug resistant recurrences. Fifteen percent of tumors carry mutations in BRCA1 or BRCA2, rendering them vulnerable treatment with PARP inhibitors such as olaparib. Recent studies have shown that TGFβ can induce “BRCAness” BRCA wild-type cells. Given is a known driver epithelial mesenchymal transition (EMT), con-nection between EMT metastatic spread EOC...
Homologous recombination among repetitive sequences is an important mode of DNA repair in eukaryotes following acute radiation exposure. We have developed assay Saccharomyces cerevisiae that models how multiple double-strand breaks form chromosomal translocations by a nonconservative homologous mechanism, single-strand annealing, and identified the Rad52 paralog, Rad59, as factor. show through genetic molecular analyses Rad59 possesses distinct Rad52-dependent -independent functions, plays...
TWIST1 is a transcription factor that necessary for healthy neural crest migration, mesoderm development, and gastrulation. It functions as key regulator of epithelial-to-mesenchymal transition (EMT), process by which cells lose their polarity gain the ability to migrate. EMT often reactivated in cancers, where it strongly associated with tumor cell invasion metastasis. Early work on adult tissues focused its transcriptional targets how gave rise metastatic cells. In recent years, roles...
Epithelial ovarian cancer (EOC) remains the most lethal gynecologic malignancy, largely due to metastasis and drug resistant recurrences. Fifteen percent of tumors carry mutations in BRCA1 or BRCA2, rendering them vulnerable treatment with PARP inhibitors such as olaparib. Recent studies have shown that TGFβ can induce “BRCAness” BRCA wild-type cells. Given is a known driver epithelial mesenchymal transition (EMT), connection between EMT metastatic spread EOC other cancers, we asked if alter...
Most cancer deaths result from tumor cells that have metastasized beyond their tissue of origin, or developed drug resistance. Across many types, patients with advanced stage disease would benefit a novel therapy preventing reversing these changes. To this end, we investigated the unique WR domain transcription factor TWIST1, which has been shown to play role in driving metastasis and resistance.In study, identified evolutionarily well-conserved residues within TWIST1 used alanine...
Oral squamous cell carcinoma (OSCC) is the most common form of head and neck cancers that predominantly affects men accounts for over 50,000 new cancer cases annually in United States. Survival rates are markedly different localized OSCC versus metastatic disease, which five-year survival only 39%. Depending on pathology stage at diagnosis, treatment may involve surgery, radiation, targeted therapy, or conventional chemotherapy. However, there an unmet need reliable biomarkers to predict...
Abstract Breast cancer is the 2nd leading cause of related deaths among women in US with over 240,000 diagnoses and 40,000 expected 2014. Among more serious deadly forms breast are Triple Negative Cancers (TNBC) (ER-, PR-, HER2-). Mortality rates patients rise dramatically when these cancers spread beyond primary tumor site. Therefore reduction cell dispersion a key component to minimizing mortality rates. Epithelial-Mesenchymal Transition (EMT) process by which cells downregulate proteins...
Abstract PURPOSE: To determine the functional effects of resveratrol analog, pterostilbene (PTE), and its potential adjuvant role with cisplatin, in ovarian uterine cancer cells. EXPERIMENTAL DESIGN: The human cell lines (OVCAR8, SKOV3) (HEC1A, ECC1) were obtained from ATCC, grown according to standard protocols using recommended media. Standard proliferation (MTT) migration assays performed routine fashion, or without PTE +/- cisplatin treatment. For in-vitro Cell Proliferation Assay,...
Abstract Introduction: Epithelial ovarian cancer (EOC) is particularly deadly due to its fast rate of growth, dissemination, and chemoresistant recurrences. Novel therapies are urgently needed limit metastasis restore platinum sensitivity. We have investigated the role TWIST1, a basic helix-loop-helix transcription factor, in these processes. TWIST1 well-documented regulator from solid tumors, which has been linked stem cell phenotypes, drug resistance, poor prognosis several tumor types....
Abstract INTRODUCTION/PURPOSE: The vast majority of epithelial ovarian cancer (EOC) patients present with advanced metastatic disease, but will respond well to first line chemotherapy consisting a platinum drug and/or paclitaxel. Unfortunately, most these relapse disease that is both and resistant, leading five-year survival rate under 20%. Therefore, novel therapies can eliminate disseminated chemoresistant cells are urgently needed. Two potential targets supplement current treatment TWIST1...