A5007804227- Cancer-related Molecular Pathways
- MicroRNA in disease regulation
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Ovarian cancer diagnosis and treatment
- Cancer, Lipids, and Metabolism
- Cancer Research and Treatments
- Cancer Cells and Metastasis
- Immunotherapy and Immune Responses
- Cancer, Hypoxia, and Metabolism
- Nanoparticles: synthesis and applications
- Connective Tissue Growth Factor Research
- RNA Research and Splicing
- Extracellular vesicles in disease
- Molecular Biology Techniques and Applications
- Cancer-related gene regulation
- Virus-based gene therapy research
- Cancer Immunotherapy and Biomarkers
- Circular RNAs in diseases
- Genetics and Neurodevelopmental Disorders
- Kruppel-like factors research
- Air Quality and Health Impacts
- Renal and related cancers
- Healthcare and Environmental Waste Management
- Cell death mechanisms and regulation
Wayne State University
2020-2024
The Barbara Ann Karmanos Cancer Institute
2022-2024
Yale University
2016-2020
University of New Haven
2020
UNSW Sydney
2011-2012
Exposure to fetal bovine serum (FBS) is shown herein reduce the aggregate size of titanium dioxide (TiO(2)) nanoparticles, affecting uptake and consequent effect on A549 H1299 human lung cell lines. Initially, cellular FBS-treated TiO(2) was lower than that non-FBS-treated TiO(2). Expulsion particles then observed, followed by a second phase TiO(2), resulting in an increase content eventually exceeding amount cells exposed Surface adsorbed vitronectin clathrin-mediated endocytosis pathway...
Abstract Epithelial–mesenchymal transition (EMT) is a critical process involved in cancer metastasis and chemoresistance. Twist1 key EMT-inducing transcription factor, which upregulated multiple types of cancers has been shown to promote tumor cell invasiveness support progression. Conversely, p53 suppressor gene that frequently mutated cancers. This study demonstrates the ability wild-type (WT) degradation protein. By forming complex with E3 ligase Pirh2, WT promotes ubiquitination...
A successful modification of titanium dioxide (TiO2) nanoparticles surfaces by a grafting-to polymer technique combining catalytic chain transfer and thiol–ene click chemistry is reported. Vinylic end functional polymers were first prepared polymerization (CCTP) using oligo(ethylene glycol) methacrylate as monomer. The presence vinylic groups was then exploited to attach the thiol functionalized TiO2 via Michael nucleophilic reactions. X-ray photoelectron spectroscopy (XPS), attenuated total...
Cancer progression, invasiveness, and metastatic potential have been associated with the activation of cellular development program known as epithelial-to-mesenchymal transition (EMT). This process is to yield not only mesenchymal cells, but instead an array cells different degrees epithelial phenotypes high plasticity, usually referred E/M hybrid cells. The characteristics their importance in tumor key regulators microenvironment that support this phenotype are still poorly understood.In...
Chromobox protein homolog 7 (CBX7), a member of the Polycomb repressor complex, is potent epigenetic regulator and gene silencer. Our group has previously reported that CBX7 functions as tumor suppressor in ovarian cancer cells its loss accelerated formation carcinomatosis drove progression an mouse model. The goal this study to identify specific signaling pathways microenvironment down-regulate CBX7. Given adipocytes are integral component peritoneal cavity microenvironment, we hypothesize...
Cancer progression requires the acquisition of mechanisms that support proliferative potential and metastatic capacity. MNRR1 (also CHCHD2, PARK22, AAG10) is a bi-organellar protein in mitochondria can bind to Bcl-xL enhance its anti-apoptotic function, or respiratory chain complex IV (COX IV) increase mitochondrial respiration. In nucleus, it act as transcription factor promote expression genes involved biogenesis, migration, cellular stress response. Given regulate both apoptosis...
Introduction Ovarian cancer recurs in most High Grade Serous Cancer (HGSOC) patients, including initial responders, after standard of care. To improve patient survival, we need to identify and understand the factors contributing early or late recurrence therapeutically target these mechanisms. We hypothesized that HGSOC, response chemotherapy is associated with a specific gene expression signature determined by tumor microenvironment. In this study, sought determine differences immune...
Ovarian cancer accounts for most deaths from gynecologic malignancies. Although more than 80% of patients respond to first-line standard care, these responders present with recurrence and eventually succumb carcinomatosis chemotherapy-resistant disease. To improve patient survival, new modalities must, therefore, target or prevent recurrent Here we describe the first time a novel syngeneic mouse model high-grade serous ovarian (HGSOC), which allows immunotherapeutic interventions in course...
Ovarian cancer is the deadliest gynecologic malignancy. The omentum plays a key role in providing supportive microenvironment to metastatic ovarian cells as well immune modulatory signals that allow tumor tolerance. However, we have limited models closely mimic interaction between and adipose-rich tissues. To further understand cellular molecular mechanisms by which provides pro-tumoral microenvironment, developed unique 3D ex vivo model of cell-omentum interaction. Using human omentum, are...
Sialylation, the addition of negatively charged sialic acid sugars to terminal ends glycans, is upregulated in most cancers. Hypersialylation supports multiple pro-tumor mechanisms such as enhanced migration and invasion, resistance apoptosis immune evasion. A current gap knowledge lack understanding on how tumor microenvironment regulates cancer cell sialylation. The adipose niche a main component peritoneal cancers' microenvironment. This includes ovarian (OC), which causes deaths from all...
Ovarian and other peritoneal cancers have a strong tendency to metastasize into the surrounding adipose tissue. This study describes an effect of microenvironment on upregulation sialic acid-containing glycans in ovarian cancer (OC). Heterogeneous populations glycosylated OC tumors converged highly sialylated cell state that regulates tumorigenesis immune-dependent manner. We modeled by conditioning growth media with human patient-derived lines grown presence vs. absence conditioned (ACM)...
Background: Epithelial-mesenchymal transition (EMT) is a biological process where epithelial cells lose their adhesive properties and gain invasive, metastatic, mesenchymal properties. Maintaining the balance between stage essential for tissue homeo-stasis. Many of genes promoting transformation has been identified; however, our understanding responsible maintaining phenotype limited. Our objective was to identify in-hibiting EMT. Methods: RNA seq performed using an vitro model CTGF...
Background: Epithelial–mesenchymal transition (EMT) is a biological process where epithelial cells lose their adhesive properties and gain invasive, metastatic, mesenchymal properties. Maintaining the balance between stage essential for tissue homeostasis. Many of genes promoting transformation have been identified; however, our understanding responsible maintaining phenotype limited. Our objective was to identify inhibiting EMT. Methods: RNA seq performed using an vitro model CTGF...
Abstract Background: Tumor metastasis is the primary cause of mortality in patients with advanced ovarian cancer. The molecular mechanism behind cancer still not clear. Twist1 associated increased ability migration and tumor-initiation cells; therefore, understanding regulation Twist critical for prevention metastatic disease. Previous studies have shown an interaction between tumor suppressor p53. We propose that p53 functions as a normal inhibitor Twist1. hypothesize through their...
<p>TP53 mutation status and protein levels of p53 Twist1 ovarian cancer cell lines. * u = unknown.</p>
<p>Expression of p53-associated E3 ligase in cancer cell lines. (A) RNA expression MDM2, Pirh2, and E6-AP1, TOPORS assessed by QPCR R182 (p53high/Twist-) Tara (p53low/Twist+) cells. GADPH was used as reference gene. (B) Protein Pirh2 Western blot. β-actin loading control.</p>
<p>The effects of Î"b-Twist1 overexpression on spheroids formation and migration. (A) Representative images spheroid forming assay. (B) the migrated cells in migration (C) Quantification cells.*P< 0.05 compared to control group.</p>
<p>The protein levels of Twist1 (A) and p53 (B) in tumor samples. Protein were determined by the quantification western blot band intensity comparing to GAPDH bands as loading control.</p>
<p>Mdm2 does not interact with Twist1. (A) Mdm2 and Twist1 were transfected in HEK293T. Western blot was used to detected the effect of on protein level. (B) immunoprecipitated HEK293T cells that plasmids. p53 IP product by western blot. product.</p>