A5007540741- Nanoparticles: synthesis and applications
- Viral Infectious Diseases and Gene Expression in Insects
- Monoclonal and Polyclonal Antibodies Research
- Nutrition and Health in Aging
- GDF15 and Related Biomarkers
- Protein purification and stability
- Macrophage Migration Inhibitory Factor
- Metalloenzymes and iron-sulfur proteins
- Nanoparticle-Based Drug Delivery
- Microbial bioremediation and biosurfactants
- Electrocatalysts for Energy Conversion
- Advanced Polymer Synthesis and Characterization
- RNA Interference and Gene Delivery
- Microfluidic and Bio-sensing Technologies
- Bacteriophages and microbial interactions
- Glycosylation and Glycoproteins Research
- Coral and Marine Ecosystems Studies
- Chemical Synthesis and Analysis
- Biodiesel Production and Applications
- Animal Genetics and Reproduction
- Healthcare and Environmental Waste Management
- T-cell and B-cell Immunology
- Advanced Nanomaterials in Catalysis
- Nanoplatforms for cancer theranostics
- Graphene and Nanomaterials Applications
UNSW Sydney
2015-2024
EMBL Australia
2018
The University of Sydney
1989-2017
Westmead Institute for Medical Research
2017
AgroParisTech
2011
The work investigates the source of toxicity copper oxide nanoparticles (CuO NPs) with respect to its leaching characteristic and speciation. Complexation-mediated CuO NPs by amino acids was identified as toward Escherichia coli, model microorganism used in current study. leached copper-peptide complex induces a multiple-fold increase intracellular reactive oxygen species generation reduces fractions viable cells, resulting overall inhibition biomass growth. cytotoxicity leachate is however...
Macrophage inhibitory cytokine-1 (MIC-1/GDF15) is a member of the TGF-b superfamily, previously studied in cancer and inflammation. In addition to regulating body weight, MIC-1/GDF15 may be used predict mortality and/or disease course cancer, cardiovascular (CVD), chronic renal heart failure, as well pulmonary embolism. These data suggested that marker all-cause mortality. To determine whether serum estimation predictor mortality, we examined cohort 876 male subjects aged 35-80 years,...
Upon contact with plasma or other protein-containing biological fluids, the surface of nanoparticles is immediately decorated proteins forming a biologically active protein corona. The fates and functions are determined by physiological responses toward these nanoparticle–protein corona complexes as effective unit nanoparticles. In this article, we review representative studies on effects particle physicochemical characteristics along profiles in medium formation importantly, how dynamic...
Macrophage inhibitory cytokine-1 (MIC-1/GDF15) is associated with cardiovascular disease, inflammation, body weight regulation and cancer. Its serum levels facilitate the diagnosis prognosis of cancer vascular disease. Furthermore, its are a powerful predictor all-cause mortality, suggesting fundamental role in biological processes ageing. In cancer, data available suggest that MIC-1/GDF15 antitumorigenic, but this may not always be case as disease progresses. Cancer promoting effects due,...
The TGF-b superfamily cytokine MIC-1/GDF15 circulates in all humans and when overproduced cancer leads to anorexia/cachexia, by direct action on brain feeding centres. In these studies we have examined the role of physiologically relevant levels regulation appetite, body weight basal metabolic rate. gene knockout mice (MIC-1−/−) weighed more had increased adiposity, which was associated with spontaneous food intake. Female MIC-1−/− exhibited some additional alterations reduced energy...
Reversible antimicrobial photoswitching of nanosilver on a semiconductor support using facile and optically selective approach is demonstrated. The non-intrusive cyclical irradiation by UV-A (300–400 nm) visible light (>450 result in controlled oxidation state dynamics the nanosilver, which turn allows its activity to be switched off repeatedly (see image). Detailed facts importance specialist readers are published as "Supporting Information". Such documents peer-reviewed, but not...
Exposure to fetal bovine serum (FBS) is shown herein reduce the aggregate size of titanium dioxide (TiO(2)) nanoparticles, affecting uptake and consequent effect on A549 H1299 human lung cell lines. Initially, cellular FBS-treated TiO(2) was lower than that non-FBS-treated TiO(2). Expulsion particles then observed, followed by a second phase TiO(2), resulting in an increase content eventually exceeding amount cells exposed Surface adsorbed vitronectin clathrin-mediated endocytosis pathway...
Macrophage inhibitory cytokine-1 (MIC-1/GDF15) modulates food intake and body weight under physiological pathological conditions by acting on the hypothalamus brainstem. When overexpressed in disease, such as advanced cancer, elevated serum MIC-1/GDF15 levels lead to an anorexia/cachexia syndrome. To gain a better understanding of its actions brainstem we studied induced neuronal activation identified induction Fos protein. Intraperitoneal injection human mice activated neurons area postrema...
Abstract Polymersomes provide a good platform for targeted drug delivery and the creation of complex (bio)catalytically active systems research in synthetic biology. To realize these applications requires both spatial control over encapsulation components polymersomes means to report where are polymersomes. address twin challenges, we synthesized protein–polymer bioconjugate PNIPAM‐ b ‐amilFP497 composed thermoresponsive poly( N ‐isopropylacrylamide) (PNIPAM) green‐fluorescent protein...
In this era of increasing antibiotic resistance, the use alternative antimicrobials such as silver has become more widespread. Superior antimicrobial activity been provided through fabrication nanoparticles or nanosilver (NAg), which imparts cytotoxic actions distinct from those bulk silver. wake recent discoveries bacterial resistance to NAg and its rising incorporation in medical consumer goods wound dressings dietary supplements, we argue that there is an urgent need monitor prevalence...
The use of a nonviral magnetic vector, comprised iron oxide nanoparticles (MNP), polyethylenimine (PEI), and plasmid DNA, for transfection BHK21 cells under field is presented. Four different vector configurations were studied by systematically varying the mixing order MNP, PEI, DNA. assembly has significant effects on its size, surface charge, cellular uptake, level gene expression. Mixing MNP with PEI first improved stability, giving narrow aggregate size distribution positive charge at...
The synthesis of well-defined functional nanoparticles for the encapsulation hydrophobic and hydrophilic drugs is described. Nanoparticles were built from amphiphilic copolymers consisting P(OEG-A) homopolymers chain extended with vinyl benzyl chloride (VBC) pentafluorophenyl acrylate (PFP-A) comonomers. Subsequently, pendant chlorine atoms, introduced into chains by VBC units, substituted using sodium methanethiosulfonate, yielding copolymer methanethiosulfonate (MTS) functionality....
Background. Elevated macrophage inhibitory cytokine-1 (MIC-1/GDF15) levels in serum mediate anorexia and weight loss some cancer patients similarly elevated occur chronic kidney disease (CKD). Serum MIC-1/GDF15 is also inflammatory diseases predicts atherosclerotic events independently of traditional risk factors. The relationship between inflammation, decreasing body mass index (BMI) increased mortality CKD not well understood being actively investigated. may link these features CKD....
The natural ability of Bacillus sp. to adapt nanosilver cytotoxicity upon prolonged exposure is reported for the first time. combined adaptive effects resistance and enhanced growth are induced under various intensities nanosilver-stimulated cellular oxidative stress, ranging from only minimal redox imbalance lethal levels ROS stimulation. An important implication present work that such lead ultimate domination nanosilver-resistant in microbiota, which continuously applied. As a service our...
The human and mouse antibody repertoires are formed by identical processes, but like all small animals, mice only have sufficient lymphocytes to express a part of the potential repertoire. In this study, we determined how heavy chain two strains generated. Analysis IgM- IgG-associated VDJ rearrangements generated high-throughput sequencing confirmed presence 99 functional immunoglobulin variable (IGHV) genes in C57BL/6 genome, inferred 164 IGHV BALB/c genome. Remarkably, five sequences were...
The TGF-b superfamily cytokine MIC-1/GDF15 circulates in the blood of healthy humans. Its levels rise substantially cancer and other diseases this may sometimes lead to development an anorexia/cachexia syndrome. This is mediated by a direct action on feeding centres hypothalamus brainstem. More recent studies germline gene deleted mice also suggest that play role physiological regulation energy homeostasis. To further characterize homeostasis man, we have examined diurnal food associated...
The work describes the interactions of nanosilver (NAg) with bacterial cell envelope components at a molecular level and how this associates reactive oxygen species (ROS)-mediated toxicity nanoparticle. Major structural changes were detected in biomolecules as result damages functional moieties, such saccharides, amides, phosphodiesters. NAg exposure disintegrates glycan backbone major wall component peptidoglycan, causes complete breakdown lipoteichoic acid, disrupts phosphate-amine fatty...
DNA methylation is a critical molecular mark involved in cellular differentiation and cell-specific processes. Single-cell whole genome profiling methods hold great potential to resolve the profiles of individual cell-types. Here we present method that couples single-cell combinatorial indexing (sci) with enzymatic conversion (sciEM) unmethylated cytosines. The sciEM facilitates single-cells highly correlated bisulfite-based workflows (r2 > 0.99) whilst improving sequencing alignment rates,...
The order of assembly a magnetic nanoparticle (MNP) vector comprised the same components (MNP, PEI, and plasmid DNA) on entry mechanism, intracellular localization, viability BHK21 cells was investigated. Cellular uptake measurements under four different inhibiting conditions, such as low temperature, depleted cellular ATP, nystatin treatment, hypertonic environment, show that mechanism MNP mediated via clathrin endocytosis. Despite component assembly, all vectors were taken up by through...