A5026207221- GDF15 and Related Biomarkers
- Nutrition and Health in Aging
- Cancer-related gene regulation
- Electrocatalysts for Energy Conversion
- Genetic Syndromes and Imprinting
- Macrophage Migration Inhibitory Factor
- Microbial bioremediation and biosurfactants
- Epigenetics and DNA Methylation
- Wnt/β-catenin signaling in development and cancer
- Muscle Physiology and Disorders
- Metalloenzymes and iron-sulfur proteins
- Chemical Synthesis and Analysis
- Connective tissue disorders research
- Microbial metabolism and enzyme function
- Algal biology and biofuel production
- Microbial Community Ecology and Physiology
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Hydrogen Storage and Materials
- Autoimmune Bullous Skin Diseases
- RNA modifications and cancer
- Biochemical Analysis and Sensing Techniques
- Kidney Stones and Urolithiasis Treatments
- Single-cell and spatial transcriptomics
- Chemical Analysis and Environmental Impact
- Silk-based biomaterials and applications
UNSW Sydney
2015-2024
Galapagos (France)
2008-2009
DNA methylation is a critical molecular mark involved in cellular differentiation and cell-specific processes. Single-cell whole genome profiling methods hold great potential to resolve the profiles of individual cell-types. Here we present method that couples single-cell combinatorial indexing (sci) with enzymatic conversion (sciEM) unmethylated cytosines. The sciEM facilitates single-cells highly correlated bisulfite-based workflows (r2 > 0.99) whilst improving sequencing alignment rates,...
Tropomyosin (Tpm) is an α helical coiled-coil dimer that forms a co-polymer along the actin filament. Tpm involved in regulation of actin's interaction with binding proteins as well stabilization filament and its assembly kinetics. Recent studies show multiple isoforms also define functional properties distinct populations within cell. Subtle structural variations conserved are key to their specificity. Therefore, we purified characterized comprehensive set 8 (Tpm1.1, Tpm1.12, Tpm1.6,...
Imine reductases (IREDs) have been identified as an important class of biocatalysts to synthesize chiral amines with substantial promise for industrial application. Here, we report the promiscuous imine reductase activity a glucose dehydrogenase (GDH). Starting from enzyme GDH Rd1bb, commercial variant typically used NAD(P)H regeneration, eight rounds directed evolution were convert this into highly active IRED chemo- and stereoselective manufacture neprilysin inhibitor precursor, improved...
Soluble hydrogenases (SH) are enzymes that catalyse the oxidation of molecular hydrogen. The SH enzyme from Cupriavidus necator H16 is relatively oxygen tolerant and makes an attractive target for potential application in biochemical hydrogen fuel cells. Expression can be mediated by derepression hox promoter system under heterotrophic conditions. However, overall impact derepression, a transcriptomic perspective, has never been previously reported.Derepression hydrogenase gene expression...
Summary We report herein the purification of a chloroform ( CF )‐reducing enzyme, TmrA, from membrane fraction strict anaerobe Dehalobacter sp. strain UNSWDHB to apparent homogeneity with an approximate 23‐fold increase in relative purity compared crude lysate. The obtained by ultracentrifugation was solubilized Triton X‐100 presence glycerol, followed anion exchange chromatography. molecular mass purified TmrA determined be 44.5 kD SDS ‐ PAGE and MALDI‐TOF/TOF. dehalogenase reductively...
Growth Differentiation Factor-15 (GDF15) is a divergent TGF-beta superfamily cytokine that overexpressed by most cancers and induced anticancer therapy. Transgenic animal models suggest it protects from cancer development but the mechanisms are uncertain. We investigated role of immunity in GDF15 reduction prostate (PCa) growth. The C57BL/6 transgenic TRAMP prone mice were bred with immunodeficient and/or systemically GDF15. developed novel orthotopic PCa model which primary tumor cells...
Reductive dehalogenases (RDases) are key enzymes involved in the respiratory process of anaerobic organohalide respiring bacteria (ORB). Heterologous expression RDases is desirable for structural and functional studies; however, there few reports successful these enzymes. Dehalobacter sp. strain UNSWDHB an ORB, whose preferred electron acceptor chloroform. This study describes efforts to express recombinant reductive dehalogenase (TmrA), derived from UNSW DHB, using heterologous hosts...
The soluble hydrogenase (SH) from Ralstonia eutropha H16 is a promising candidate enzyme for H2-based biofuel application as it favours H2 oxidation and relatively oxygen-tolerant. In this report, bioprocess development studies undertaken to produce purify an active SH are described, based on the methods previously reported [1], [2], [3], [4]. Our modifications are: •Upstream method optimizations were heterotrophic growth media cell lysis involving ultrasonication.•Two anion exchangers (Q...
Abstract The pemphigoid disease epidermolysis bullosa acquisita (EBA) is an autoimmune blistering skin characterized by autoantibodies against type VII collagen (COL7), immune cell infiltrates at the dermal–epidermal junction and subepidermal blistering. Proteases, particularly granzyme B (GzmB), have been established as therapeutic targets for treatment of EBA other diseases. We investigated impact novel GzmB inhibitor SNT‐6935 on anti‐COL7 IgG‐induced in a well‐established ex vivo model....
Drug resistance among parasitic nematodes has resulted in an urgent need for the development of new therapies. However, high re-discovery rate anti-nematode compounds from terrestrial environments necessitates a repository future drug research. Marine epiphytes are hypothesised to produce nematicidal as defence against bacterivorous predators, thus representing promising yet underexplored source discovery. The marine epiphytic bacterium Pseudoalteromonas tunicata is known several bioactive...
Abstract DNA methylation is a critical molecular mark involved in cellular differentiation and cell-specific processes. Single-cell whole genome profiling methods hold great potential to resolve the profiles of individual cell-types. Here we present method that couples single-cell combinatorial indexing (sci) with enzymatic conversion (sciEM) unmethylated cytosines. The facilitates single-base resolution single-cells highly correlated bisulfite-based workflows (r 2 >0.99) whilst improving...