To address an important challenge in the engineering of antioxidant nanoparticles, present work devised a surface-to-bulk migration oxygen vacancies radical-scavenging cerium-oxide nanoparticles. The study highlights significance surface intended cellular internalization and, subsequently, radical scavenging activity nanoparticles inside cells. findings advise future development therapeutic nanomaterials to also include particles for enhanced defects not only accessibility their but also,...

The work describes the interactions of nanosilver (NAg) with bacterial cell envelope components at a molecular level and how this associates reactive oxygen species (ROS)-mediated toxicity nanoparticle. Major structural changes were detected in biomolecules as result damages functional moieties, such saccharides, amides, phosphodiesters. NAg exposure disintegrates glycan backbone major wall component peptidoglycan, causes complete breakdown lipoteichoic acid, disrupts phosphate-amine fatty...

Treatment of bacterial biofilms are difficult and in many cases, expensive. Bacterial naturally more resilient to antimicrobial agents than their free-living planktonic counterparts, rendering the community growth harder control. The present work described risks long-term use an important alternative antimicrobial, silver nanoparticles (NAg), for first time, on dominant mode growth.NAg could inhibit formation as well eradicating already grown biofilm Pseudomonas aeruginosa, a pathogen...

The increasing biological use of graphene-based materials has prompted research inquiries on their effects microorganisms. work herein reported different types microbiological activity reduced graphene oxide (RGO). At relatively high concentrations (200 and 400 μg/mL), RGO exhibited antibacterial the model bacterium Escherichia coli, while at lower (10 50 interestingly, no effect was observed. Instead, an increase in viable population after exposure observed, verified by colony counting...

This research explores the adaptive defense mechanisms of a nanosilver-resistant pathogen (NAg R ) to protect and fight off complex antimicrobial targeting silver nanoparticles. The Gram-negative bacterium Acinetobacter baumannii upregulated expression outer membrane proteins for cell surface defense, as well capsule synthesis genes. Increased abundance surface-attached biofilm colonies in NAg was linked phenotypically indicated increase integrity, with also forming more EPS, biopolymer...

Flow cytometry, using fluorescein-bound specific antibodies and propidium iodide, was shown to be effective in detecting Legionella spp. cooling tower waters. The procedure quicker less labor intensive than fluorescent microscopy. use of these procedures also identified qualitative differences, perhaps related infectivity, populations.

To date, efforts to study the resistance mechanisms of carbapenem-resistant Acinetobacter baumannii have been largely focused on two major globally distributed clones (GC1 and GC2). ST85 is an emerging sequence type, unlike other clones, it associated with carriage bla NDM gene.

The present work describes the evolution of a resistance phenotype to multitargeting antimicrobial agent, namely, silver nanoparticles (nanosilver; NAg), in globally prevalent bacterial pathogen Acinetobacter baumannii. Gram-negative bacterium has recently been listed as critical priority requiring novel treatment options by World Health Organization. Through prolonged exposure important nanoparticle, developed mutations genes that encode protein subunits organelle structures are involved...

Available DNA isolation methods for Plasmodium involve numerous processing steps, adding to the cost and conferring risk of contamination. Here we devise a simple cost-effective method direct extraction from dried filter paper spot (DBS), appropriate resource-limited setups.The protocol involves freezing thawing DBS, neither any purification step nor chemical reagent. The was assessed in terms quantity, PCR detection sensitivity, time requirement, effectiveness, labor intensiveness degree...

Precise cell division in coordination with DNA replication and segregation is of utmost importance for all organisms. The earliest stage the assembly a protein FtsZ into ring, known as Z at midcell. What still eludes us, however, how bacteria precisely position ring Work B. subtilis over last two decades has identified link between early stages division. A recent model proposed that progression leads to an increased ability form This arose through studies examining mutants blocked different...

Abstract Objectives To determine the genetic context of genes conferring antibiotic resistance on carbapenem-resistant Acinetobacter baumannii Cl415, recovered in 2017 at El Youssef Hospital Centre Akkar Governorate, North Lebanon. Methods Antibiotic phenotype for 22 antibiotics was determined using disc diffusion or MIC determination. The whole-genome sequence Cl415 a combination Illumina MiSeq and Oxford Nanopore (MinION) platforms. Complete genome assembled Unicycler determinants ISs were...

The antimicrobial properties of copper are well-known but maintaining a low oxidation state Cu in particles is difficult. Herein, CuxP were synthesized through phosphorization Cu(OH)2, to lock its monovalent (as Cu3P). We found that the could be achieved at temperatures as 200°C, with stable surface presence Cu(I) on resulting particles. can act one-electron reducing agent for molecular oxygen, generate highly reactive hydroxyl radical. In this study, displayed antibacterial activities...

Bacteria are present everywhere—all around and within us. Are you scared of them? Do not be, because most bacteria actually good for Only a small number them can occasionally cause infections, making us sick. infections by dividing quickly inside the human body, that is, process one cell into two cells at fast pace. To fuel growth division, need to find their favorite food be able (digest) it correctly. Like humans love eat candies, choices is simple sugar called glucose. We have found when...