Abstract Plant innate immune response to pathogen infection includes an elegant signaling pathway leading reactive oxygen species generation and resulting hypersensitive (HR); localized programmed cell death in tissue surrounding the initial site limits spread. A veritable symphony of cytosolic molecules (including Ca2+, nitric oxide [NO], cyclic nucleotides, calmodulin) have been suggested as early components HR signaling. However, specific interactions among these secondary messengers...

Consumer demands for foods promoting health while preventing diseases have led to development of functional that contain probiotic bacteria. Fermented dairy products are good substrates delivery, but the large number lactose intolerant people, their high fat and cholesterol content also due growing vegetarianism consumers seeking alternatives. Therefore, researches been widely studied feasibility bacteria in non-dairy such as fruits, vegetables, cereals. This review describes application...

The phytopathogenic bacterium Pantoea stewartii subsp. synthesizes stewartan exo/capsular polysaccharide (EPS) in a cell density-dependent manner governed by the EsaI/EsaR quorum-sensing (QS) system. This study analyzes biofilm development and host colonization of WT QS regulatory mutant strains P. stewartii. First, we show that synthesis EPS, system, is required for proper bacterial adhesion spatially defined, 3D biofilms. Second, nonvirulent lacking esaI gene adheres strongly to surfaces...

The saprophytic yeast-like fungus Aureobasidium pullulans has been well documented for over 60 years in the microbiological literature. It is ubiquitous distribution, being found a variety of environments (plant surfaces, soil, water, rock surfaces and manmade surfaces), with worldwide distribution from cold to warm climates wet/humid regions arid ones. Isolates strains A. produce wide range natural products international literature which have regarded as safe biotechnological environmental...

The distinct sensorial characteristics of local cheeses influence consumer preferences, and make an essential contribution to the economy. Microbial diversity in cheese is among fundamental contributors qualitative characteristics. However, knowledge regarding existence microbial patterns associated with regional production practices ripened remains limited. present research was conducted test hypothesis that background metagenome could be used as a marker their origin. We compared Irish...

Wine production in Cyprus has strong cultural ties with the island’s tradition, influencing local and foreign consumers’ preferences contributing significantly to Cyprus’ economy. A key contributor wine quality sensorial characteristics development is microbiota that colonizes grapes performs alcoholic fermentation. Still, microbial patterns of wines produced different geographic regions ( terroir ) remain unknown. The present study investigated diversity five terroirs Cyprus, two from PGI...

Table olives are one of the most well-known traditionally fermented products and their global consumption is exponentially increasing. In directly brining, table produced spontaneously, without any debittering pre-treatment. Up to date, fermentation process remains empirical inconstant, as it affected by physicochemical attributes fruit, tree fruit management pro post-harvest, influencing also natural microbiota on olive drupes. present study, whole cracked Picual were at industrial scale...

Τable olive is one of the main fermented vegetable worldwide and can be processed as treated or natural product. Lactic Acid Bacteria (LAB) are responsible for fermentation olives. The aim this work was to study technological characteristics potential probiotic properties LAB isolated from Cypriot green table This first comprehensive report on isolation characterization isolates retrieved From a collection 92 spontaneously fermenting olives, 64 g positive were firstly identified genus level...

Table olives are one of the most established Mediterranean vegetables, having an exponential increase consumption year by year. In natural-style processing, produced spontaneous fermentation, without any chemical debittering. This natural fermentation process remains empirical and variable since it is strongly influenced physicochemical parameters microorganism presence in olive drupes. present work, Cypriot green cracked table were processed directly brine (natural olives), using three...

The knowledge about the microbial diversity of different olives varieties from diverse regions in Mediterranean basin is limited. This work aimed to determine three fermented olive varieties, collected Cyprus, via Next Generation Sequencing (NGS) analysis. Olives were spontaneously for 120 days, DNA was extracted final products, and subjected 16S rRNA gene ITS1 loci metabarcoding analysis determination bacterial fungal communities, respectively. Results revealed that profile studied similar,...

Potato cyst nematodes (PCN), Globodera pallida and rostochiensis , are obligate parasites of solanaceous plants, causing severe losses in several potato growing areas throughout the world. To date, management PCN is related to nematode population densities estimated as eggs per gram soil, without considering actual number viable juveniles within cysts. In classical nematology, standard method determine viability based on a staining assay, using Meldola's blue dye (MB) followed by microscopic...

In general, it is a common practice among dairy producers to store the milk in refrigerator directly after milking, order preserve and prevent development of spoilage microbes. However, impact keeping overnight on microbial diversity has been poorly investigated. This study aimed provide snapshot bacterial composition goat direct storage at −80 °C being kept 4 then °, using high-throughput sequencing (HTS). Goat samples from four different farms were analyzed, reveal that differed between...

Human food-borne diseases caused by pathogenic bacteria have been significantly increased in the last few decades causing numerous deaths worldwide. The standard analyses used for their detection significant limitations regarding cost, special facilities and equipment, highly trained staff, a long procedural time that can be crucial foodborne pathogens with high hospitalization mortality rates, such as Listeria monocytogenes. This study aimed to develop biosensor could detect L....

Sweet basil (Ocimum basilicum L.) is an economically important annual aromatic plant, grown mostly for culinary use both fresh and dry consumption as a source of essential oil. In Cyprus, approximately 4 ha are annually, either in greenhouses year-round crop or open fields from April to November, the majority production exported European market. During May 2012, sweet cv. Genovese Gigante greenhouse operation area Limassol was severely affected by foliar disease, causing almost 100% losses....

A new laboratory technique combining shoot‐tip grafting in vitro and biological indexing on indicator plants was explored for the detection of citrus exocortis related viroids. Τhree methods were used compared with classical method. With vivo method, diagnosis is based expression symptoms indicators 11–14 weeks after inoculation. In contrast, first microindexing citron seedlings by graft inoculation, possible 12 days inoculation; second cuttings 20 third injection 40 Inoculated Etrog...

Cyprus traditional sausages from the Troodos mountainous region of Pitsilia gained protected geographical indication (PGI) designation European Committee (EU 2020/C 203/06). Still, we lack authentication protocols for distinction “Pitsilia” industrially produced sausages. Microbial activity is an essential contributor to sausages’ sensorial characteristics, but whether microbial patterns might be associated with area production unclear. In present research, applied high-throughput sequencing...