A5037692738- Plant Pathogens and Fungal Diseases
- Fungal Plant Pathogen Control
- Plant Pathogens and Resistance
- Plant Disease Resistance and Genetics
- Mycotoxins in Agriculture and Food
- Plant-Microbe Interactions and Immunity
- Yeasts and Rust Fungi Studies
- Mycorrhizal Fungi and Plant Interactions
- Forest Insect Ecology and Management
- Plant Disease Management Techniques
- Essential Oils and Antimicrobial Activity
- Entomopathogenic Microorganisms in Pest Control
- Phytochemistry and Biological Activities
- Nematode management and characterization studies
- Postharvest Quality and Shelf Life Management
- Plant and Fungal Interactions Research
- Parasite Biology and Host Interactions
- Nanocomposite Films for Food Packaging
- Cocoa and Sweet Potato Agronomy
- Pomegranate: compositions and health benefits
- Water Treatment and Disinfection
- Plant Stress Responses and Tolerance
- Genetic diversity and population structure
- Powdery Mildew Fungal Diseases
- Insect behavior and control techniques
Cyprus University of Technology
2009-2022
Benaki Phytopathological Institute
2015
Agricultural University of Athens
2015
University of California, Riverside
2007-2010
North Carolina State University
2009
Fusarium circinatum, the causal agent of pine pitch canker (PPC), is currently one most important threats Pinus spp. globally. This pathogen known in many pine-growing regions, including natural and planted forests, can affect all life stages trees, from emerging seedlings to mature trees. Despite importance PPC, global distribution F. circinatum poorly documented, this problem also true hosts within countries that are affected. The aim study was review with a particular focus on Europe. We...
Three new fungicides (i.e., azoxystrobin, fludioxonil, and pyrimethanil) are currently being introduced for postharvest management of citrus green mold in the United States. The effectiveness each fungicide was evaluated when applied alone (at 1,000 to 1,200 mg/liter) or mixtures 500 mg/liter component) lemon fruit that were wound-inoculated with imazalil/thiabendazole (TBZ)-sensitive -resistant isolates Penicillium digitatum. In laboratory studies aqueous solutions 9 21 h after inoculation,...
Abstract This article documents the addition of 512 microsatellite marker loci and nine pairs Single Nucleotide Polymorphism (SNP) sequencing primers to Molecular Ecology Resources Database. Loci were developed for following species: Alcippe morrisonia , Bashania fangiana, fargesii Chaetodon vagabundus Colletes floralis Coluber constrictor flaviventris Coptotermes gestroi Crotophaga major Cyprinella lutrensis Danaus plexippus Fagus grandifolia Falco tinnunculus Fletcherimyia fletcheri,...
Genetic and biochemical mechanisms of fludioxonil pyrimethanil resistance in isolates Penicillium digitatum were evaluated compared to those characterized other fungi. Resistant naturally occurring packinghouses not associated with crop losses. For the phenylpyrrole fludioxonil, EC50 values 0.02 0.04 microg/ml for sensitive, 0.08 0.65 moderately resistant (MR), > 40 highly (HR) isolates. Two fludioxonil-sensitive also significantly more sensitive unrelated dicarboximide fungicide iprodione,...
Plant pathogenic fungi are considered of significant economic importance for adversely affecting both quantitatively and qualitatively fresh processed produce. Extracts Salvia fruticosa were initially screened their antifungal activity, the ethyl acetate fraction, being most active, was further analyzed using HPLC-SPE-NMR hyphenation. The methoxylated flavones hispidulin, salvigenin, cirsimaritin diterpenes carnosic acid, carnosol, 12-methoxycarnosic acid identified as major components...
Grapevine trunk diseases (GTDs) are widespread worldwide, causing serious economic losses to the vitiviniculture industry. The etiology of complex pathogenic mycobiome associated with this group is critical implementing appropriate management strategies. Diseased grapevines exhibiting typical GTD symptoms were collected from vineyards in different provinces Cyprus, resulting 19 pycnidial isolates. Subsequent multilocus sequence analysis 6 genetic loci (ITS, LSU, SSU, b-tub, tef1-a, and rpb2)...
A new technique, the spiral gradient dilution method, was evaluated for determining 50% effective concentrations (EC 50 values) of fungicides inhibition mycelial growth and conidial germination various fungi. In this an agar medium is plated with a fungicide solution by means plater, which applies in 2.5-log continuous radial concentration gradient. Fungal inoculum then placed along lines across After incubation plates, distinct shapes were observed different fungus-fungicide interactions....
Three new fungicides, azoxystrobin, fludioxonil, and pyrimethanil, that belong to different chemical classes are highly effective in managing citrus green mold being registered for postharvest use the United States. Recirculating in-line drenches provided a significantly improved efficacy compared with standard low-volume spray applications. To prevent pathogen contamination of drench solutions, two oxidizing disinfectants, sodium hypochlorite hydrogen peroxide/peroxyacetic acid (HPPA) were...
Consumer concerns regarding high-quality produce, free of pesticide residues, direct research towards disease management strategies that minimise or even exclude the use synthetic chemistries in crop production. The efficacy a chitosan-based edible coating combined with acetonic extract Salvia fruticosa Mill. (ASF) was assessed against grey mould table grapes.HPLC-SPE-NMR and q-NMR analyses defined major constituents ASF to be flavonoids hispidulin, salvigenin cirsimaritin diterpenes...
Biotic and abiotic stresses, such as fungal infection drought, cause major yield losses in modern agriculture. Kresoxim-methyl (KM) belongs to the strobilurins, one of most important classes agricultural fungicides displaying a direct effect on several plant physiological developmental processes. However, impact KM treatment salt drought stress tolerance is unknown. In this study we demonstrate that pre-treatment Medicago truncatula plants results increased protection stress. Foliar...
For the first time in over 25 years, three new fungicides (azoxystrobin, fludioxonil, and pyrimethanil), all belonging to different chemical classes, are being registered for postharvest use against Penicillium decays of citrus fruit United States. Baseline sensitivities digitatum P. italicum were developed using isolates collected before commercial these fungicides. In a comparison methods, EC50 values obtained spiral gradient dilution method very similar those traditional agar dilutions...
Abstract Fusarium circinatum is a harmful pathogenic fungus mostly attacking Pinus species and also Pseudotsuga menziesii , causing cankers in trees of all ages, damping-off seedlings, mortality cuttings mother plants for clonal production. This listed as quarantine pest several parts the world trade potentially contaminated pine material such cuttings, seedlings or seeds restricted order to prevent its spread disease-free areas. Inspection plant often relies on DNA testing conventional...
ABSTRACT Fungicide resistance was identified in natural populations of Penicillium digitatum, the causal agent green mold citrus, to two three new postharvest fungicides before their commercial use. Using a air-sampling method where large pathogen citrus packinghouses were exposed agar plates with continuous, wide-range fungicide concentration gradient, isolates reduced sensitivity fludioxonil or pyrimethanil obtained. Resistance frequencies and calculated as 9.5 x 10(-7) 1.5 10(-5) 7.3...
To determine the effects of solar radiation on survival sporangia Pseudoperonospora cubensis , cause cucurbit downy mildew, detached P. were either exposed to sun or placed in shade from 08·00 17·00 h sunny and cloudy days at Raleigh, North Carolina, experiments conducted weekly 9 July 8 October 2008. Sporangia assessed for germination every hour following exposure was normalized based percentage unexposed same collection. Exposure a 9‐h period, shade, significantly ( P < 0·0001) reduced...
Potato cyst nematodes (PCN), Globodera pallida and rostochiensis , are obligate parasites of solanaceous plants, causing severe losses in several potato growing areas throughout the world. To date, management PCN is related to nematode population densities estimated as eggs per gram soil, without considering actual number viable juveniles within cysts. In classical nematology, standard method determine viability based on a staining assay, using Meldola's blue dye (MB) followed by microscopic...
Sweet basil (Ocimum basilicum L.) is an economically important annual aromatic plant, grown mostly for culinary use both fresh and dry consumption as a source of essential oil. In Cyprus, approximately 4 ha are annually, either in greenhouses year-round crop or open fields from April to November, the majority production exported European market. During May 2012, sweet cv. Genovese Gigante greenhouse operation area Limassol was severely affected by foliar disease, causing almost 100% losses....
During 2011, 96 sclerotial isolates of Rhizoctonia solani were collected from potato tubers all main potato-cultivating regions Cyprus. All found to be multinucleate. Characterization anastomosis groups (AG) based on hyphal reactions showed that 91 belonged AG3 and 5 AG4. Sequence analysis the internal transcribed spacer (ITS) (ITS1 ITS2) ribosomal DNA (rDNA) 68 confirmed prevalence AG3. In addition, phylogenetic type, distinctly separated tobacco while AG4 into two different subgroups (HGI...
We evaluated spray coverage on grape leaves and bunches by a high-volume sprayer (HVS), an air-assisted, low volume knapsack (LVS), motorized dust applicator modified to liquid formulations (common – CS). At the full development of vine canopy 1400, 250 150 L ha−1 were applied via HVS, CS LVS, respectively. Sprayer type, leaf or bunch orientation, position side significantly affected (P < 0.001). Mean was below 21% for CS, above 40 55% LVS Downy mildew infection 5% throughout season, with...
Besides well-known grapevine trunk disease (GTD)-related pathogens, there is an increased interest in wood-colonizing fungi that infect grapevines. During 2017-2018, a survey was conducted Cyprus and wood samples were collected from vines exhibiting typical GTD symptoms. Based on morphological multilocus phylogenetic analyses (ITS, LSU, bt2, tef1-a), four species the Sporocadaceae family described typified; two genus of Seimatosporium: Seim. cyprium sp. nov. vitis-viniferae Sporocadus: Spo....