A5046891424- Bacterial biofilms and quorum sensing
- Antibiotic Resistance in Bacteria
- Nanoparticles: synthesis and applications
- Streptococcal Infections and Treatments
- Image Processing Techniques and Applications
- Cell Image Analysis Techniques
- Graphene and Nanomaterials Applications
- Tracheal and airway disorders
- Inhalation and Respiratory Drug Delivery
- Antimicrobial Resistance in Staphylococcus
- Enterobacteriaceae and Cronobacter Research
- Neonatal and Maternal Infections
- Phytochemicals and Antioxidant Activities
- Gut microbiota and health
- Bacterial Identification and Susceptibility Testing
- Phytochemistry and Biological Activities
- Advanced Drug Delivery Systems
- Urinary Tract Infections Management
- Nosocomial Infections in ICU
- Advanced Nanomaterials in Catalysis
- Orthopedic Infections and Treatments
- Pneumonia and Respiratory Infections
- Clostridium difficile and Clostridium perfringens research
- Airway Management and Intubation Techniques
- Bacteriophages and microbial interactions
Jagiellonian University
2014-2024
Czech Academy of Sciences, Institute of Microbiology
2020
Faculty (United Kingdom)
2020
The interaction between metal nanoparticles and bacteria belongs to the central issues in a dynamically growing bionanotechnological research. Herein, we investigated adhesion efficiency of gold (30 nm) for various bacterial strains, both Gram-positive (Bacillus subtilis, Staphylococcus carnosus) Gram-negative (Neisseria subflava, Stenotrophomonas maltophilia). thorough microscopic (SEM/TEM) observations revealed that do not penetrate into cells but adhere walls. Large differences adhered...
In microbiology it is diagnostically useful to recognize various genera and species of bacteria. It can be achieved using computer-aided methods, which make the recognition processes more automatic thus significantly reduce time necessary for classification. Moreover, in case diagnostic uncertainty (the misleading similarity shape or structure bacterial cells), such methods minimize risk incorrect recognition. this article, we apply state art method texture analysis classify This uses deep...
Non-cytotoxic, temperature-responsive and antibacterial poly(di(ethylene glycol)methyl ether methacrylate) - POEGMA188 based nanocomposite coatings attached to a glass surface were successfully prepared using ATRP polymerization. The thickness, morphology wettability of the resulting analyzed ellipsometry, AFM contact angle measurements, respectively. strong impact thicknesses grafted brush content AgNPs on temperature-induced changes was demonstrated. In addition temperature-dependent...
In this work we have investigated the effect of oxygen plasma treatment graphenic surfaces and introduction functional groups on changes in function, wettability, surface free energy bacterial adhesion. The parameters were adjusted (generator power: <60 W, exposure time: <20 min) to limit modifications without changing bulk structure. parent modified thoroughly characterized by μRaman spectroscopy, thermogravimetry, scanning electron microscopy, contact angle, X-ray photoelectron function...
(1) Background: Due to the commonness of tracheotomy procedures and wide use biomaterials in form tracheostomy tubes (TTs), problem biomaterial-associated infections (BAIs) is growing. Bacterial colonization TTs results development biofilms on surface biomaterials, which may contribute invasive tracheostomized patients. (2) Methods: Clinical strains K. pneumoniae, isolated from TTs, were characterized according their ability biofilms, as well resistance antibiotics, whether they harbored...
Abstract Background The aim of this study was to investigate the epidemiology and resistance methicillin-resistant Staphylococcus aureus (MRSA) isolates from long-term care facilities (LTCF) residents analyze potential risk factors for MRSA occurrence, defined as colonization and/or infection. Methods Point prevalence (PPS) prospective incidence continuous (CS) carried out on a group 193 in 2009-2010. Results Overall occurred (with or without infection) among 17.6% residents. There 16 cases...
Lactobacilli play an important role in maintaining vaginal health and protection against bacterial infections the genital tract. The aim of this study is to show dynamics changes rectal Lactobacillus flora during pregnancy by using Sanger sequencing method. included 31 healthy pregnant women without clinical signs genitourinary infections. material was taken three trimesters swabs grown on MRS agar quantitatively estimate number spp. [CFU/ml]. Afterwards, 3 8 morphologically different...
Bone infections are a serious problem to cure, as systemic administration of antibiotics is not very effective due poor bone vascularization. Therefore, many drug delivery systems investigated solve this problem. One the potential solutions from poly(L-actide-co-glycolide) (PLGA) nanoparticles suspended in gellan gum injectable hydrogel. However, loading capacity and release kinetics system based on hydrophilic drugs (e.g., gentamycin) hydrophobic polymers PLGA) may always be satisfying. To...
Background: Bacterial biofilm on the surface of tracheostomy tubes (TTs) is a potential reservoir potentially pathogenic bacteria, including S. aureus. For this reason, our study aimed to investigate production in vitro and presence icaAD MSCRAMM genes clinical aureus strains derived from TTs, with respect antibiotic resistance genetic variability. Methods: The clonality was analyzed by PFGE method. assessment drug based EUCAST recommendations. isolates were evaluated for microtiter plate...
Implant-related infections are a worldwide issue that is considered very challenging. Conventional therapies commonly end up failing; thus, new solutions being investigated to overcome this problem. The in situ delivery of the drug at implant site appears be more sufficient compared systemic antibiotic therapy. In study, we manufactured porous zirconia scaffolds using foam replication method. To improve their overall bioactivity, they were coated with calcium phosphate (CaP) layer containing...
Ionizing radiation is used as a therapeutic option in the treatment of certain neoplastic lesions located, among others, pelvic region. The doses employed often result adverse effects manifesting themselves primarily form genital tract infections patients or diarrhea. data available literature indicate disorders microbial ecosystem caused by ionizing radiation, which leads to problems mentioned above. In present study, we examined influence on 52 selected strains bacteria: Lactobacillus...
Abstract Carbapenem-resistant Klebsiella pneumoniae (CRKP) is an important bacterium of nosocomial infections. In this study, CRKP strains, which were mainly isolated from fecal samples 14 patients in three wards the hospital Silesia Voivodship, rapidly increased February to August 2018. Therefore, we conducted microbiological and molecular studies isolates analyzed. Colonized had critical underlying diseases comorbidities; one developed bloodstream infection, five died (33.3%). Antibiotic...
Group B streptococcus (GBS) is one of the uropathogens that causes urinary tract infections (UTIs). The aims this article were molecular characterization, an analysis antimicrobial susceptibility profiles, adherence to bladder endothelial cells, and detection immunoreactive proteins 94 clinical strains GBS isolated from adult Polish patients with UTI. Antibiotic susceptibilities determined by disk diffusion. Serotyping Alp family genes studied using multiplex PCR. Genetic profiles...
The aim of the study was a retrospective analysis frequency group B streptococci (Streptococcus agalactaie; GBS) carriage in pregnant women from region Krakow, together with an their drug resistance, carried out between 2008-2012. included 3363 35 and 37 weeks gestation, studied accordance guidelines Polish Gynecological Society (2008). A high percentage who are carriers demonstrated. Each year covered by study, it range 25-30%, average value equal to 28%. results confirm need for taking...
Abstract The objective of the study was to perform a comparative analysis phenotypic and genetic similarity, determination resistance profiles, detection toxin-encoding genes molecular typing Clostridium perfringens isolates originating from patients with gas gangrene. encompassed three clinical microbiological diagnosis gangrene who were hospitalized in one hospitals Kujawsko-Pomorskie province same period time between 8 th April 2015 20 2015. C. perfrin- gens studied had identical...
Preliminary microbiological diagnosis usually relies on microscopic examination and, due to the routine culture and bacteriological examination, lasts up 11 days. Hence, many deep learning methods based images were recently introduced replace time-consuming examination. They shorten by 1–2 days but still require iterative obtain monoculture samples. In this work, we present a feasibility study for further shortening time analyzing polyculture images. It is possible with multi-MIL, novel...
<b>Introduction:</b> In hospitalized patients, tracheostomy tubes (TTs) are susceptible to colonization by biofilm- producing potentially pathogenic microorganisms (PPMs). Contact with TTs, which situated in a critical region of the body enormous microbial exposure, may lead emer-gence resistant respiratory infections.</br></br> <b>Objective:</b> Our study aimed isolate and identify Gram-positive Gram-negative PPMs, mark their antibiotic resistance...
The interaction between bacteria and nanoparticles is currently a central topic in bionanotechnology.
In this work, we automatically distinguish between different clones of the same bacteria species (Klebsiella pneumoniae) based only on microscopic images. It is a challenging task, previously seemed unreachable due to high clones' similarity. For purpose, apply multi-step algorithm with attention-based deep multiple instance learning, which returns parts image crucial prediction. Except for obtaining accuracy, introduce extensive explainability persistence homology, increasing...
Solid lipid nanoparticles are currently one of the most widely investigated types drug delivery carriers. Considering fact that their clinical translation boosted after approval two COVID-19 mRNA vaccines, it is crucial to fully explain how processing parameters affect properties obtained and loading efficiency. The following study aimed evaluate influence different on stearic acid based fabricated using emulsification/solvent diffusion method. It was found type organic solvent used has a...
Abstract Gram-negative bacilli Klebsiella pneumoniae are among the most important pathogens responsible for healthcare-associated infections (HAIs). These bacteria often have high pathogenic and epidemic potential, contributing to infection outbreaks worldwide. K. is part of natural microbiota humans. At same time, as an opportunistic microorganism, when host organism weakened, it can cause serious such pneumonia, urinary tract infections, septic intra-organ abscesses. Widespread...