A5013913026- Pancreatic and Hepatic Oncology Research
- Cancer Genomics and Diagnostics
- IL-33, ST2, and ILC Pathways
- Immune Cell Function and Interaction
- MicroRNA in disease regulation
- Diabetes and associated disorders
- Epigenetics and DNA Methylation
- Cancer Immunotherapy and Biomarkers
- Meteorological Phenomena and Simulations
- Glycosylation and Glycoproteins Research
- Immune cells in cancer
- Precipitation Measurement and Analysis
- Chemical Synthesis and Analysis
- Colorectal Cancer Treatments and Studies
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Single-cell and spatial transcriptomics
- Atmospheric chemistry and aerosols
- Cancer Research and Treatments
- Immunotherapy and Immune Responses
- Mechanical Circulatory Support Devices
- Pancreatitis Pathology and Treatment
- Atmospheric aerosols and clouds
- Enzyme Structure and Function
- Sirtuins and Resveratrol in Medicine
- Building Energy and Comfort Optimization
University of Michigan–Ann Arbor
2009-2025
Michigan Medicine
2022
University of Pittsburgh Medical Center
1995-2020
University of Pittsburgh
2014-2018
Children's Hospital of Pittsburgh
2017
Colorado State University
2000-2005
Med Center
1996
Abstract The adult healthy human pancreas has been poorly studied given the lack of indication to obtain tissue from in absence disease and rapid postmortem degradation. We obtained pancreata brain dead donors, thus avoiding any warm ischemia time. 30 donors were diverse age race had no known disease. Histopathologic analysis samples revealed pancreatic intraepithelial neoplasia (PanIN) lesions most individuals irrespective age. Using a combination multiplex IHC, single-cell RNA sequencing,...
Abstract Pancreatic cancer is characterized by an extensive fibroinflammatory microenvironment. During carcinogenesis, normal stromal cells are converted to cytokine-high cancer-associated fibroblasts (CAF). The mechanisms underlying this conversion, including the regulation and function of fibroblast-derived cytokines, poorly understood. Thus, efforts therapeutically target CAFs have so far failed. Herein, we show that signals from epithelial expressing oncogenic KRAS—a hallmark pancreatic...
The aryl hydrocarbon receptor (AhR) is a ubiquitous nuclear with broad range of functions, both in tumor cells and immune within the microenvironment (TME). Activation AhR has been shown to have carcinogenic effect variety organs, through induction cellular proliferation migration, promotion epithelial-to-mesenchymal transition, inhibition apoptosis, among other functions. However, impact on cell function more complicated, pro- anti-tumorigenic roles identified. Although targeting cancer...
Aims: Mitochondrial stress and dysfunction within the intestinal epithelium are known to contribute pathogenesis of inflammatory bowel disease (IBD). However, importance mitophagy during inflammation remains poorly understood. The primary aim this study was investigate how protein BCL2/adenovirus E1B 19 kDa protein-interacting 3-like (BNIP3L/NIX) mitigates mitochondrial damage in hopes that these data will allow us target health as an adjunct immune-based treatment strategies. Results: In...
703 Background: Pancreatic ductal adenocarcinoma (PDAC) is the third-leading cause of cancer death in United States with a 5-year survival rate 13%. Recurrence rates are high following resection and optimization neoadjuvant adjuvant therapy remains under investigation, hypothesis that neoadjuvant/perioperative may convert borderline resectable tumors to prevent progression micro-metastases postoperative setting. Our study evaluates patient outcomes models predictive prognostic markers assist...
Structural and thermodynamic analysis of a family synthetic proteins with heterogeneous backbones yields new insights into the ability unnatural amino acids to be accommodated α-helices.
Introduction We have previously demonstrated that a pathologic downregulation of peroxisome proliferator-activated receptor–gamma coactivator 1-alpha (PGC1α) within the intestinal epithelium contributes to pathogenesis inflammatory bowel disease (IBD). However, mechanism underlying PGC1α expression and activity during IBD is not yet clear. Methods Mice (male; C57Bl/6, Villincre /+; Pgc1afl/fl mice, ) were subjected experimental colitis treated with nicotinamide riboside. Western blot,...
Pancreatic ductal adenocarcinoma (PDAC) is a devastating disease with 5-year survival rate of 12.5%. PDAC predominantly arises from non-cystic pancreatic intraepithelial neoplasia (PanIN) and cystic intraductal papillary mucinous neoplasm (IPMN). We used multiplex immunofluorescence computational imaging technology to characterize, map, compare the immune microenvironments (IMEs) its precursor lesions. demonstrate that IME IPMN was abundantly infiltrated CD8+ T cells PD-L1-positive...
The mimicry of protein tertiary structure by oligomers with unnatural backbones is a significant contemporary research challenge. Among common elements secondary found in natural proteins, sheets have proven the most difficult to address. Here, we report systematic comparison different strategies for peptide backbone modification β-sheets goal identifying best method replacing multi-stranded sheet fold. effective modifications examined led native-like folding behavior thermodynamic folded...
Abstract Significant controversy surrounds the adaptive infrared iris hypothesis put forth by Lindzen et al., whereby tropical anvil cirrus detrainment is hypothesized to decrease with increasing sea surface temperature (SST). This dependence would act as an iris, allowing more radiation escape into space and inhibiting changes in temperature. assumes that increased precipitation efficiency regions of higher temperatures will reduce detrainment. Tropical Rainfall Measuring Mission (TRMM)...
The “ Wakasa Bay Experiment” was conducted in order to refine error models for oceanic precipitation from the Advanced Microwave Sounding Radiometer-Earth Observing System (AMSR-E) measurements and develop algorithms snowfall. NASA P-3 aircraft equipped with microwave radiometers, covering a frequency range of 10.7–340 GHz, radars at 13.4, 35.6, 94 deployed Yokota Air Base Japan flights 14 January 3 February 2003. For four flight days (27–30 January) Gulfstream II provided by Core Research...
The incidence and prevalence of inflammatory bowel disease (IBD) are increasing worldwide. IBD is known to be multifactorial, but signaling within the intestinal epithelium a subsequent failure epithelial barrier have been shown play essential roles in pathogenesis. CaMKIV multifunctional protein kinase associated with inflammation cell cycle regulation. has extensively studied autoimmune diseases, role idiopathic not described. In this study, active was highly expressed humans ulcerative...
Despite advances in therapy over the past decades, metastatic colorectal cancer (mCRC) remains a highly morbid disease. While impact of MHC-I on immune infiltration mCRC has been well studied, data consequences MHC-II loss are lacking. Multiplex fluorescent immunohistochemistry (mfIHC) was performed 149 patients undergoing curative intent resection for and stratified into high low human leukocyte antigen isotype DR (HLA-DR) expressing tumors. Intratumoral HLA-DR expression found stromal...
Introduction Metastatic colorectal cancer (mCRC) remains a common and highly morbid disease, with recent increase in incidence patients younger than 50 years. There is an acute need to better understand differences tumor biology, molecular characteristics, other age-related the microenvironment (TME). Methods 111 undergoing curative-intent resection of liver metastases were stratified by age into those <50 years or >65 old, tumors subjected multiplex fluorescent...
<p>Supplementary Figures 11-14 and associated legends.</p>
<div>Abstract<p>The adult healthy human pancreas has been poorly studied given lack of indication to obtain tissue from the in absence disease and rapid postmortem degradation. We obtained pancreata brain dead donors thus avoiding any warm ischemia time. The 30 were diverse age race had no known disease. Histopathological analysis samples revealed PanIN lesions most individuals irrespective age. Using a combination multiplex immunohistochemistry, single cell RNA sequencing,...
<div>Abstract<p>The adult healthy human pancreas has been poorly studied given lack of indication to obtain tissue from the in absence disease and rapid postmortem degradation. We obtained pancreata brain dead donors thus avoiding any warm ischemia time. The 30 were diverse age race had no known disease. Histopathological analysis samples revealed PanIN lesions most individuals irrespective age. Using a combination multiplex immunohistochemistry, single cell RNA sequencing,...
<p>Supplementary Figures 1-10 and associated legends</p>
<p>Supplementary Figures 1-10 and associated legends</p>
<p>Supplementary Figures 11-14 and associated legends.</p>