A5050587638- Cytomegalovirus and herpesvirus research
- Herpesvirus Infections and Treatments
- Toxoplasma gondii Research Studies
- Immune Cell Function and Interaction
- interferon and immune responses
- Viral-associated cancers and disorders
- Immune Response and Inflammation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- T-cell and B-cell Immunology
- HIV Research and Treatment
- Viral gastroenteritis research and epidemiology
- 3D Printing in Biomedical Research
- Animal Disease Management and Epidemiology
- Virus-based gene therapy research
- Respiratory viral infections research
- Immune cells in cancer
- Click Chemistry and Applications
- Erythrocyte Function and Pathophysiology
- Phagocytosis and Immune Regulation
- Mycobacterium research and diagnosis
- Cell Image Analysis Techniques
- FOXO transcription factor regulation
- Cellular Mechanics and Interactions
- Influenza Virus Research Studies
- Cancer Cells and Metastasis
University of Arizona
2017-2024
Louisiana State University Health Sciences Center Shreveport
2013-2018
Louisiana State University Health Sciences Center New Orleans
2012
ABSTRACT In the United States (US), biosafety and biosecurity oversight of research on viruses is being reappraised. Safety in virology paramount frameworks should be reviewed periodically. Changes made with care, however, to avoid impeding science that essential for rapidly reducing responding pandemic threats as well addressing more common challenges caused by infectious diseases. Decades uniquely positioned US able respond COVID-19 crisis astounding speed, delivering life-saving vaccines...
The establishment of human cytomegalovirus (HCMV) latency and persistence relies on the successful infection hematopoietic cells, which serve as sites viral contribute to spread. Here, using blocking antibodies pharmacological inhibitors, we document that HCMV activation epidermal growth factor receptor (EGFR) downstream phosphatidylinositol 3-kinase (PI3K) mediates entry into CD34
We have established that HCMV acts as a specific ligand engaging and activating cellular integrins on monocytes. As result, integrin signaling via Src activation leads to the functional of paxillin required for efficient viral entry biological changes in monocytes needed dissemination. These biological/molecular allow use "vehicles" systemic spread establishment lifelong persistence. However, it remains unresolved how specifically induces this observed monocyte activation. It was previously...
Reactivation from latency requires reinitiation of viral gene expression and culminates in the production infectious progeny. The major immediate early promoter (MIEP) human cytomegalovirus (HCMV) drives crucial lytic cycle transactivators but is silenced during hematopoietic progenitor cells (HPCs). Because MIEP has poor activity HPCs, it unclear how are expressed reactivation. It been presumed that reinitiated via de-repression MIEP. We demonstrate transcripts arising reactivation...
The ability of human cytomegalovirus (HCMV) to reactivate from latent infection hematopoietic progenitor cells (HPCs) is intimately linked cellular differentiation. HCMV encodes UL7 that our group has shown secreted infected and induces angiogenesis. In this study, we show a ligand for Fms-like tyrosine kinase 3 receptor (Flt-3R), well-known critical factor in HPC We observed directly binds Flt-3R downstream signaling cascades, including phosphatidylinositol 3-kinase (PI3K)/Akt...
Innate immune responses are crucial for limiting virus infection. However, viruses often hijack our best defenses viral objectives. Human Cytomegalovirus (HCMV) is a beta herpesvirus which establishes life-long latent Defining the virus-host interactions controlling latency and reactivation vital to control of disease risk posed by reactivation. We defined an interaction between UL138, pro-latency HCMV gene, host deubiquitinating complex, UAF1-USP1. UAF1 scaffold protein pivotal activity...
Significance Human cytomegalovirus (HCMV) infection is lifelong and persistent. Periodic reactivation of poses serious disease risk for immune-compromised patients. A critical driver expression viral genes from the major immediate early locus. Recent paradigm-shifting evidence shows that driven promoters distinct those drive replication in permissive cells. Understanding contextual control these how they specifically respond to cellular cues establishing future therapies prevent...
ABSTRACT Monocytes play a key role in the hematogenous dissemination of human cytomegalovirus (HCMV) to target organ systems. To infect monocytes and reprogram them deliver infectious virus, HCMV must overcome biological obstacles, including short life span their antiviral proapoptotic response infection. We have shown that virally induced upregulation cellular Mcl-1 promotes early survival HCMV-infected monocytes, allowing cells an apoptotic checkpoint at around 48 h postinfection (hpi)....
Human cytomegalovirus (HCMV) infects peripheral blood monocytes and triggers biological changes that promote viral dissemination persistence. We have shown HCMV induces a proinflammatory state in infected monocytes, resulting enhanced monocyte motility transendothelial migration, prolonged survival, differentiation toward long-lived M1-like macrophage phenotype. Our data indicate these changes, the absence of de novo gene expression replication, through engagement activation epidermal growth...
Significance Human cytomegalovirus (HCMV)-infected monocytes play a critical role in the hematogenous dissemination of virus, establishment persistence, and progression HCMV-mediated diseases. Here we identify unique HCMV nuclear translocation pathway monocytes. moves from early endosomes to trans-Golgi network, then recycling before its translocation. Signaling (via c-Src) induced by viral binding gH/gL/UL128-131 complex integrins triggers this pathway. Because is degraded without signal,...
Human cytomegalovirus (HCMV) is a beta herpesvirus that persists indefinitely in the human host through latent infection. The polycistronic UL133-UL138 gene locus of HCMV encodes genes regulating latency and reactivation. While UL138 pro-latency, restricting virus replication CD34+ hematopoietic progenitor cells (HPCs), UL135 overcomes this restriction required for By contrast, UL136 expressed with later kinetics multiple proteins differential roles Like UL135, largest isoform, UL136p33,...
ABSTRACT Human cytomegalovirus (HCMV) is beta herpesvirus that persists indefinitely in the human host through a protracted, latent infection. The polycistronic UL133-UL138 gene locus of HCMV encodes genes regulating latency and reactivation. While UL138 pro-latency, restricting virus replication CD34+ hematopoietic progenitor cells (HPCs), UL135 overcomes this restriction for By contrast, UL136 expressed with later kinetics multiple protein isoforms differential roles Like UL135, largest...
Cellular motility is an important biological process for both unicellular and multicellular organisms. It essential movement of organisms towards a source nutrients or away from unsuitable conditions, as well in tissue development, immune surveillance wound healing, just to mention few roles(1,2,3). Deregulation this can lead serious neurological, cardiovascular immunological diseases, exacerbated tumor formation spread(4,5). Molecularly, actin polymerization receptor recycling have been...
Innate immune responses are crucial for limiting virus infection. However, viruses often hijack our best defenses viral objectives. Human Cytomegalovirus (HCMV) is a beta herpesvirus which establishes life-long latent Defining the virus-host interactions controlling latency and reactivation vital to control of disease risk posed by reactivation. We defined an interaction between UL138, pro-latency HCMV gene, host deubiquintase complex, UAF1-USP1. UAF1 scaffold protein pivotal activity...
Liver X receptor (LXR) signaling broadly restricts virus replication; however, the mechanisms of restriction are poorly defined. Here, we demonstrate that cellular E3 ligase LXR-inducible degrader low-density lipoprotein (IDOL) targets human cytomegalovirus (HMCV) UL136p33 protein for turnover. UL136 encodes multiple proteins differentially impact latency and reactivation. is a determinant targeted rapid turnover by proteasome, its stabilization mutation lysine residues to arginine results...