A5029149320- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Agricultural Science and Fertilization
- Crop Yield and Soil Fertility
- Agricultural pest management studies
- Viral Infections and Immunology Research
- Agricultural Economics and Practices
- Microbial infections and disease research
- Animal Disease Management and Epidemiology
- Genetic and Environmental Crop Studies
- Agronomic Practices and Intercropping Systems
- Antibiotic Resistance in Bacteria
- Agricultural Systems and Practices
- Mycobacterium research and diagnosis
- Vector-Borne Animal Diseases
- Virus-based gene therapy research
- Rice Cultivation and Yield Improvement
- Herpesvirus Infections and Treatments
- Tree Root and Stability Studies
- Vibrio bacteria research studies
- Genetics and Plant Breeding
- Plant Virus Research Studies
- Veterinary medicine and infectious diseases
- Animal health and immunology
- Agricultural Practices and Plant Genetics
National Institute of High Security Animal Diseases
2018-2024
Arizona State University
2023
Weatherford College
2022
Indian Institute of Technology Ropar
2021
Chaudhary Charan Singh Haryana Agricultural University
2021
Central Sheep and Wool Research Institute
2012-2020
University of Toronto
2016
Indian Institute of Technology Indore
2014
Indian Institute of Technology Roorkee
2010-2013
Chandra Shekhar Azad University of Agriculture and Technology
2006
African swine fever (ASF) is the most dreaded disease of pigs, which can cause mortality up to 100%. Following outbreaks with high in pigs two states north-east India, namely Arunachal Pradesh and Assam early 2020, we confirmed first occurrence domestic India by real-time PCR, virus isolation nucleotide sequencing. Genetic analyses three independent genomic regions (B646L gene encoding p72 protein, E183L p54 protein central variable region (CVR) B602L gene) showed that Indian ASF viruses are...
The study focused on developing an indigenous, one-step reverse transcription (RT)-PCR assay for detecting the porcine transmissible gastroenteritis virus (TGEV) genome. In summary, a gene construct and two sets of primers were designed by aligning N sequences from various TGEV strains, which subsequently synthesized. was sub-cloned into pTZ57R/T vector, enabling synthesis in vitro transcribed (IVT) RNA, served as TGEV-positive control RT-PCR protocol optimization. optimization involved...
African swine fever (ASF), considered as the most dreadful disease due to its very high mortality, emerged in India 2020. The complete genome analysis of ASF viruses isolated during first outbreaks showed a few unique non-synonymous mutations MGF 369-11L, 505-4R, K205R and B263R genes. Frame shifts protein coding sequences were observed DP60R, ASFV-G_ACD 00190, 110-10-L-MGF110-14L fusion, 360-14L I267L genes Indian compared ASFV/Georgia/2007. Complete based phylogenetic p72-genotype-II...
Post-transcriptional regulation of mRNAs plays an essential role in the control gene expression. are regulated ribonucleoprotein (RNP) complexes by RNA-binding proteins (RBPs) along with associated protein and noncoding RNA (ncRNA) cofactors. A global understanding post-transcriptional any cell type requires identification components all its RNP complexes. We have previously shown that these can be purified immunoprecipitation using anti-RBP synthetic antibodies produced phage display. To...
Present study was carried out to know the seroprevalence of BHV-1 in population cattle and buffaloes from Chhattisgarh, India. A total 464 serum samples were collected different districts Chhattisgarh. The screened by Avidin-Biotin Enzyme Linked Immunosorbent Assay kit that recorded an overall 34.69%. Out 422 samples, 158 (37.44%) found positive compared 3 (7.14%) 42 buffaloes. In age groups, there variability prevalence BHV-1. Animals above 9 years showed highest seropositivity (45.9%)...
The present study was planned to detect the genetic elements of Mannheimia haemolytica and Pasteurella multocida in pneumonic sheep lungs. Pneumonia diagnosed on basis gross pathological lesions. Lung tissues were collected at necropsy (n = 96) subjected isolation total DNA. M. haemolytica-specific PHSSA Rpt2 genes, P. multocidaspecific KMT1 Omp87 genes amplified using polymerase chain reaction (PCR). A housekeeping gene targeting cellular mitochondrial 12S ribosomal DNA used as an internal...
Porcine reproductive and respiratory syndrome (PRRS) is an economically important transboundary viral disease of pigs confronting the swine industry worldwide. This study was aimed to assess pathogenic potential PRRS virus belonging genotype 2 that emerged in India 2013. Nine 6-week-old piglets were inoculated intranasally with × 105.75 TCID50/ml PRRSV (Ind-297221/2013). Three kept as uninfected controls. Blood nasal swabs collected daily up 7 days post-infection (dpi) on alternate...
The present study was planned to isolate Escherichia coli from pneumonic and septicemic sheep goats determine antimicrobial resistance (AMR) patterns of the isolates evaluate them for presence extended-spectrum ß-lactamase (ESBL) using phenotypic molecular methods. Pneumonic lung tissues heart blood samples were collected by performing necropsy on (n = 96) 08). processed used bacterial isolation. E. 58) including 53 34 5 3 recovered identified cultural biochemical characteristics 16S rRNA...
Objective of the present study was to isolate bovine herpes virus Type 1 (BHV-1) from semen infected bull and adapt it onto embryonated eggs Madin-Darby kidney (MDBK) cell line. Further, identified by agar gel immunodiffusion (AGID) test.
The present study was planned to detect the genes encoding carbapenemases, ESBLs and class 1 integron-integrase among bacteria obtained from retail goat meat. Fermenting non-fermenting bacterial isolates (n = 57), recovered 61 meat samples, were identified by 16S rRNA gene sequencing. Antimicrobial susceptibility of tested broth dilution method using ceftazidime, cefotaxime, meropenem imipenem. Plasmids isolated for their physical characters. subjected screening carbapenemase, ESBL intI1...
The present study was aimed to detect the carbapenemase, extended-spectrum β-lactamase (ESBL), and intI1 gene of class 1 integron among fermenting (n = 61) nonfermenting 10) bacterial isolates recovered from water samples 128). Isolates were identified by 16S rRNA sequencing. These showed reduced-susceptibility third-generation cephalosporins carbapenems. varied in number size plasmids (2 kb >20 kb). Plasmid DNA screening 5·6, 7, 11·2 26·7% prevalence blaKPC , blaNDM blaSHV blaTEM genes...
The aim of this study was to isolate the antimicrobial-resistant enterobacteria from cattle feces and identify extended-spectrum beta-lactamase (ESBL) carbapenemase gene among them. A total 70 fecal swab samples were collected Durg, Chhattisgarh, India, cultured in nutrient broth. Bacterial isolation recovered 120 gram-negative bacteria. Antimicrobial susceptibility testing showed 90 (75%) coliform isolates (66 Escherichia coli, 20 Klebsiella sp., two each Enterobacter sp. Citrobacter sp.)...