A5103192568- Bacillus and Francisella bacterial research
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Salmonella and Campylobacter epidemiology
- Poxvirus research and outbreaks
- Bacterial Identification and Susceptibility Testing
- Escherichia coli research studies
- Virus-based gene therapy research
- Animal Disease Management and Epidemiology
- Microbial Inactivation Methods
- Antibiotic Resistance in Bacteria
- Yersinia bacterium, plague, ectoparasites research
- Leptospirosis research and findings
- HIV Research and Treatment
- Vibrio bacteria research studies
- Infant Nutrition and Health
- Glycosylation and Glycoproteins Research
- Research in Cotton Cultivation
- Vector-Borne Animal Diseases
- Humic Substances and Bio-Organic Studies
- Blood groups and transfusion
- Metabolomics and Mass Spectrometry Studies
- Animal health and immunology
ICAR - Central Institute for Research on Cotton Technology
2025
ICMR National Institute for Research in Environmental Health
2021-2023
Indian Veterinary Research Institute
2015-2023
Translational Health Science and Technology Institute
2022-2023
National Institute of High Security Animal Diseases
2016-2022
Sanjay Gandhi Post Graduate Institute of Medical Sciences
2022
Defence Research and Development Establishment
2016-2021
Centre for Development of Advanced Computing
2004
Abstract Antimicrobial resistant Klebsiella pneumoniae ( K. ), as being a pathogen of critical clinical concern, urgently demands effective therapeutic options. However, the discovery novel antibiotics over last three decades has declined drastically and necessitates exploring strategies. Metabolomic modulation been promising approach for development therapeutics to deal with AMR; however, only limited efforts have made to-date, possibly due unavailability suitable metabolites extraction...
Porcine reproductive and respiratory syndrome (PRRS) is an economically important transboundary viral disease of pigs confronting the swine industry worldwide. This study was aimed to assess pathogenic potential PRRS virus belonging genotype 2 that emerged in India 2013. Nine 6-week-old piglets were inoculated intranasally with × 105.75 TCID50/ml PRRSV (Ind-297221/2013). Three kept as uninfected controls. Blood nasal swabs collected daily up 7 days post-infection (dpi) on alternate...
Emerging antimicrobial resistance (AMR) among Gram-positive pathogens, specifically in Staphylococcus aureus (S. aureus), is becoming a leading public health concern demanding effective therapeutics. Metabolite modulation can improve the efficacy of existing antibiotics and facilitate development However, it remained unexplored for drug-resistant S. (gentamicin methicillin-resistant), primarily due to dearth optimal metabolite extraction protocols including protocol AMR-associated...
We describe the implementation of GBTK (Grid BLAST Tool Kit), a lightweight application specific grid framework for BLAST, geographically distributed high performance computing (HPC) systems. This was built on concept synchronized Web services using RPC (remote procedure calls) encoded as XML (extensible Markup Language).
Anthrax, caused by Bacillus anthracis is known to occur globally since antiquity. Besides being an important biothreat agent, it public health importance pathogen also in countries like India. B. secretes three distinct toxins, namely protective antigen (PA), lethal factor (LF) and edema (EF). PA the central moiety of anthrax toxin complex therefore has been a molecule choice for vaccine development. four different domains with functions. In this study, major were cloned expressed bacterial...
Background: Anthrax, a disease of bioterrorism and public health importance is caused by the Gram positive, spore-forming bacterium, Bacillus anthracis. Anthrax toxin, tripartite toxin composed protective antigen (PA), lethal factor (LF) or edema (EF). PA major protein which facilitates entry component either factor. Recombinant has been suitable target for anthrax vaccine worldwide. However, instead full PA, its domains are reported to provide protection. LF also contributes...
Category A classified Bacillus anthracis is highly fatal pathogen that causes anthrax and creates challenges for global security public health. In this study, development of a safe ideal next-generation subunit vaccine has been evaluated in mouse model.Protective antigen (PA) BA3338, surface layer homology (SLH) domain possessing protein were cloned, expressed heterologous system purified by IMAC. Recombinant PA BA3338 with alum administered alone or combination. The humoral cell-mediated...
Anthrax, caused by Bacillus anthracis is an important disease of biowarfare and public health importance. It imperative to develop a simple system which can detect differentiate B. from other closely related species. The surface array protein (Sap), secreted during the early growth phase bacteria be biomarker for detection anthracis. In present study, we have developed rapid flow through membrane ELISA Polyclonal antibodies were used sandwich plate ELISA, could 3.9 ng/ml recombinant Sap....
Protective antigen (PA) produced by Bacillus anthracis is a highly immunogenic protein. Therefore, it has significant importance in serodiagnosis as well vaccine candidate for anthrax. In the present study, codons PA gene were optimised and synthesised its expression Escherichia coli. Various conditions scaled up production of rPA. The final yield affinity chromatography purified protein was 40.8 mg/l during batch fermentation. For further purification, diafiltered subjected to anion...
Detection of spores Bacillus anthracis, the causative agent anthrax in human and animals environment is cumbersome due to presence other closely related species. The Extractable Antigen 1 (EA1), an extracellular protein considered as a biomarker for detection B. anthracis spores. In present work, we have cloned expressed recombinant EA1 soluble form Escherichia coli. Optimisation culture conditions cultivation media was carried out achieve enhanced expression protein. Further, batch...
Background: Anthrax, caused by Bacillus anthracis is known to occur globally since antiquity. In human, the disease manifests itself in one of three forms: cutaneous, gastrointestinal or pulmonary depending upon route spore entry. Cutaneous anthrax a public health importance also country like India. Therefore, there need develop an improved, simple, sensitive, specific, user-friendly, field usable, cost-effective and universal detection method for serodiagnosis human animals. Methods &...