A5069394638- Plant Pathogens and Fungal Diseases
- Mycorrhizal Fungi and Plant Interactions
- Advanced biosensing and bioanalysis techniques
- bioluminescence and chemiluminescence research
- Plant and fungal interactions
- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Resistance
- CRISPR and Genetic Engineering
- Genetically Modified Organisms Research
- Genetic diversity and population structure
- RNA and protein synthesis mechanisms
- Lipoproteins and Cardiovascular Health
- Biosensors and Analytical Detection
- Pharmacogenetics and Drug Metabolism
- Advanced Biosensing Techniques and Applications
- Yeasts and Rust Fungi Studies
- Species Distribution and Climate Change
- Innovative Microfluidic and Catalytic Techniques Innovation
- PARP inhibition in cancer therapy
- Microbial Natural Products and Biosynthesis
- Plant and animal studies
- Ecology and Vegetation Dynamics Studies
- Plant Ecology and Taxonomy Studies
- Cytomegalovirus and herpesvirus research
- Polyomavirus and related diseases
Biotest (Germany)
2022-2024
Goethe University Frankfurt
2015-2019
Institute of Clinical Cancer Research
2016-2018
Hellenic Telecommunications Organization (Greece)
2008
Space Hellas (Greece)
2007
National and Kapodistrian University of Athens
2003-2004
University of Patras
2003
The highly specific molecular recognition properties of oligonucleotides are combined with the unique optical gold nanoparticles for development a dry-reagent strip-type biosensor that enables visual detection double stranded DNA within minutes. assay does not require instrumentation and avoids multiple incubation washing steps performed in most current assays. Gold nanoparticle reporters oligo(dT) attached to their surface form an integral part strip. Biotinylated PCR products (233 bp or...
Root endophytic fungi are found in a great variety of plants and ecosystems, but the ecological drivers their biogeographic distribution poorly understood. Here, we investigate occurrence root endophytes non-mycorrhizal plant genus Microthlaspi, effect environmental factors geographic distance structuring communities at continental scale. We sampled 52 populations across northern Mediterranean central Europe used cultivation approach to study communities. Cultivation roots yielded 2601...
Summary There is increasing knowledge on the diversity of root‐endophytic fungi, but limited information their lifestyles and dependence hosts hampers our understanding ecological functions. We compared biogeographical patterns cultivable noncultivable root endophytes to assess whether occurrence determined by distinct factors. The endophytic in roots nonmycorrhizal Microthlaspi spp. growing across Europe was assessed using high‐throughput sequencing ( HTS ) with a previous dataset based...
Summary Fungi are prolific producers of natural products routinely screened for biotechnological applications, and those living endophytically within plants attract particular attention because their purported chemical diversity. However, the harnessing biosynthetic potential is hampered by a large often cryptic phylogenetic ecological diversity, coupled with lack large‐scale products' dereplication studies. To guide efforts to discover new chemistries among root‐endophytic fungi, we...
ABSTRACT Background Establishing the contribution of host plant genotype to assembly root-associated microbial communities can be challenging, since multiple ecological factors may confound effects purely due host, and species often do not occur sympatrically. Aims We aimed measure relative importance on driving natural root-endophytic fungal communities, as compared other habitat conditions that depend local environment. Methods Species annual wild Brassicaceae cultivated Brassica napus...
Hemoglobinopathies are the most common inherited diseases worldwide. Various methods for genotyping of hemoglobin, beta (HBB) gene mutations have been reported, but there is need a high sample-throughput, cost-effective method simultaneous screening several mutations. We report that combines detectability and dynamic range chemiluminescence with allele-discrimination ability probe extension reactions 15 HBB in dry-reagent format.We genotyped IVSI-110G>A, CD39C>T, IVSI-1G>A, IVSI-6T>C,...
Studying community structure and dynamics of plant-associated fungi is the basis for unravelling their interactions with hosts ecosystem functions. A recent sampling revealed that only a few fungal groups, as defined by internal transcribed spacer region (ITS) sequence similarity, dominate culturable root endophytic communities nonmycorrhizal Microthlaspi spp. plants across Europe. Strains these display broad phenotypic functional diversity, which suggests genetic variability masked ITS...
Plant symbioses with fungal root endophytes span a continuum from mutualistic to parasitic outcomes, and are highly variable depending on the genotype of each symbiont. The abiotic context in which interactions occur also seems influence outcome plant–endophyte symbioses, but we lack understanding its relative importance. We aimed assess if changes variables determine effects plant growth. used vitro co-cultivation assays test impact selection endophytic strains diverse lineages growth...
We report a general procedure for the preparation of biomolecular conjugates that combine molecular recognition properties oligonucleotides with high detectability photoprotein aequorin. Central to conjugation protocols is use recombinant aequorin fused hexahistidine tag. In one protocol, an amino-modified oligonucleotide was treated homobifunctional cross-linker carrying two N-hydroxysuccinimide ester groups, and derivative allowed react (His)6-aequorin. A second strategy involved...
Hepatitis B immunoglobulin (HBIG) in combination with a potent nucleos(t)ide analog is considered the standard of care for prophylaxis against hepatitis virus (HBV) reinfection after liver transplantation HBV-associated disease.