The oncoprotective role of food-derived polyphenol antioxidants has been described but the implicated mechanisms are not yet clear. In addition to polyphenols, phenolic acids, found at high concentrations in a number plants, possess antioxidant action. main acids foods derivatives 4-hydroxybenzoic acid and 4-hydroxycinnamic acid. This work concentrates on antiproliferative action caffeic acid, syringic sinapic protocatechuic ferulic 3,4-dihydroxy-phenylacetic (PAA) T47D human breast cancer...

Maternal thyroid dysfunction, especially in early pregnancy, may lead to pregnancy complications and adverse birth outcomes. Few population-based prospective studies have evaluated these effects results are discrepant. We examined the association of function autoimmunity with The study used data from mother-child cohort "Rhea" Crete, Greece. A total 1170 women singleton pregnancies participated this analysis. serum samples first trimester were tested for hormones (TSH, free T4, T3)...

Breast cancer (one of the most common malignancy in Western societies), as well esophagus, stomach, lung, bladder, and prostate cancer, depend on environmental factors diet for growth evolution. Dietary micronutriments have been proposed effective inhibitory agents initiation, progression, incidence. Among them, polyphenols, present different foods beverages, retained attention recent years. Red wine is a rich source their antioxidant tumor arresting effects demonstrated vitro vivo systems....

The effect of different wine antioxidant polyphenols (catechin, epicatechin, quercetin, and resveratrol) on the growth three prostate cancer cell lines (LNCaP, PC3, DU145) was investigated. A dose- time-dependent inhibition by found at nanomolar concentrations. proliferation LNCaP PC3 cells preferentially inhibited flavonoids quercetin), whereas resveratrol most potent inhibitor DU145 growth. Possible mechanisms action were investigated: 1) competition for androgen binding in revealed...

Abstract Estrogens are known modulators of monocyte/macrophage functions; however, the underlying mechanism has not been clearly defined. Recently, a number estrogen receptor molecules and splice variants were identified that exert different sometimes opposing actions. We assessed expression receptors explored their role in mediating estrogenic anti-inflammatory effects on human primary monocytes. report only expressed receptor-α 36-kDa variant G-protein coupled 30/G-protein 1,...

The neurosteroid dehydroepiandrosterone (DHEA), produced by neurons and glia, affects multiple processes in the brain, including neuronal survival neurogenesis during development aging. We provide evidence that DHEA interacts with pro-survival TrkA pro-death p75NTR membrane receptors of neurotrophin nerve growth factor (NGF), acting as a neurotrophic factor: (1) anti-apoptotic effects were reversed siRNA against or specific inhibitor; (2) [3H]-DHEA binding assays showed it bound to membranes...

The neuroactive steroids dehydroepiandrosterone (DHEA), its sulfate ester DHEA (DHEAS), and allopregnanolone (Allo), produced by the CNS adrenals, appear to exert a protective effect in hippocampal cortical neuron ischemia- excitotoxicity-induced injury. We hypothesized that they may also play role on adrenal medulla, an important part of sympathetic nervous system, tissue adjacent their primary site production. DHEA, DHEAS, Allo protected rat chromaffin cells pheochromocytoma PC12 cell...

Recent findings have shown that, in addition to the genomic action of steroids, through intracellular receptors, short-time effects could be mediated binding membrane sites. In present study prostate cancer LNCaP cells, we report that dihydrotestosterone and non-internalizable analog testosterone-BSA increase rapidly release prostate-specific antigen (PSA) culture medium. Membrane testosterone sites were identified ligand on preparations, flow cytometry, confocal laser microscopy fluorescent...

The human prostate cancer cell line LNCaP bears functional membrane testosterone receptors, which modify the actin cytoskeleton and increase secretion of prostate-specific antigen (PSA) within minutes. Membrane steroid receptors are, indeed, a newly identified element action that is different from classical intracellular sites. In present work, using nonpermeable analog (testosterone-BSA), we investigated signaling pathway triggered by receptors' activation leads to reorganization. We report...

Nongenomic androgen actions imply mechanisms different from the classical intracellular receptor (iAR) activation. We have recently reported identification of a membrane (mAR) on LNCaP human prostate cancer cells, mediating testosterone signal transduction within minutes. In present study we provide evidence that activation mAR by nonpermeable, BSA-coupled results in 1) inhibition cell growth (with 50% inhibitory concentration 5.08 nM, similar to affinity for sites); 2) induction cells both...

Abstract Adipose tissue represents a rich source of multipotent stem cells. Mesenchymal cells, isolated from this source, can differentiate to other cell types in vitro and therefore be used for number regenerative therapies. Our view adipose has recently changed, establishing adipocytes as new members the immune system, they produce proinflammatory cytokines (such IL-6 TNFα chemokines, addition adipokines (leptin, adiponectin, resistin) molecules associated with innate system. In paper, we...

The beneficial role of olive oil consumption is nowadays widely recognized. However, it not clear whether its health effects are due to the presence monounsaturated lipids and/or antioxidant fraction microconstituents present in oil. aim study was analyze exact modification metabolic factors (glucose and circulating lipids) explore polyphenols. In work, we have performed a network meta-analysis 30 human intervention studies, considering direct indirect interactions impact each constituent....

Abstract: In the present study we examined interaction of opiates with δ and μ opioid binding sites in bovine adrenal medulla. [ 3 H][D‐Ala 2 , D‐Leu 5 ]‐enkephalin ([ H]DADLE) presence saturating concentrations morphiceptin was used to analyze site interactions, whereas either H]DADLE [D‐Ser Leu ]‐enkephalinThr 6 (DSLET) or Me‐Phe 4 Gly ‐ol]‐enkephalin H]DAGO) for determination sites. Both were found interact stereo selectively opiates. The affected differentially by proteolytic enzymes...

To characterize the relationship of TSH receptor-adenylate cyclase system to differentiation in human thyroid cancers, adenylate and binding were studied membranes from primary metastatis carcinomas varying histological types (n = 33) normal thyroids 12). Membranes differentiated 23) exhibit wide patient variability; some show entirely TSH-binding characteristics, other decreased stimulation which is accompanied by either a or decrease site concentration. With respect concentration adenylat...

Recent studies suggest an association between chronic inflammation, modulating the tissue microenvironment, and tumor biology. Tumor environment consists of tumor, stromal endothelial cells infiltrating macrophages, T lymphocytes, dendritic cells, producing array cytokines, chemokines growth factors, accounting for a complex cell interaction regulation differentiation, activation, function survival surrounding responsible progression spreading or induction antitumor immune responses...