A5090925322- Neuropeptides and Animal Physiology
- Cancer, Stress, Anesthesia, and Immune Response
- Receptor Mechanisms and Signaling
- Microtubule and mitosis dynamics
- Sphingolipid Metabolism and Signaling
- Cellular transport and secretion
- Estrogen and related hormone effects
- Cellular Mechanics and Interactions
- Ion Transport and Channel Regulation
- Phytochemicals and Antioxidant Activities
- Cell death mechanisms and regulation
- Hormonal and reproductive studies
- Mass Spectrometry Techniques and Applications
- HER2/EGFR in Cancer Research
- Tea Polyphenols and Effects
- Protein Kinase Regulation and GTPase Signaling
- Pharmacological Receptor Mechanisms and Effects
- Protein Hydrolysis and Bioactive Peptides
- NF-κB Signaling Pathways
- Sirtuins and Resveratrol in Medicine
- Endoplasmic Reticulum Stress and Disease
- Cell Adhesion Molecules Research
- Caveolin-1 and cellular processes
- Prostate Cancer Treatment and Research
- Multiple Sclerosis Research Studies
Centre National de la Recherche Scientifique
2008-2022
Institut de Pharmacologie et de Biologie Structurale
2012-2022
Université de Toulouse
2008-2022
Université Toulouse III - Paul Sabatier
2012-2022
Laboratoire de Biologie Cellulaire et Moléculaire du Contrôle de la Prolifération
2008-2018
La Ligue Contre le Cancer
2017
Inserm
1996-2012
Centre National pour la Recherche Scientifique et Technique (CNRST)
2012
Institut Curie
2005-2012
Institute of Experimental Endocrinology of the Slovak Academy of Sciences
2006-2008
The oncoprotective role of food-derived polyphenol antioxidants has been described but the implicated mechanisms are not yet clear. In addition to polyphenols, phenolic acids, found at high concentrations in a number plants, possess antioxidant action. main acids foods derivatives 4-hydroxybenzoic acid and 4-hydroxycinnamic acid. This work concentrates on antiproliferative action caffeic acid, syringic sinapic protocatechuic ferulic 3,4-dihydroxy-phenylacetic (PAA) T47D human breast cancer...
Breast cancer (one of the most common malignancy in Western societies), as well esophagus, stomach, lung, bladder, and prostate cancer, depend on environmental factors diet for growth evolution. Dietary micronutriments have been proposed effective inhibitory agents initiation, progression, incidence. Among them, polyphenols, present different foods beverages, retained attention recent years. Red wine is a rich source their antioxidant tumor arresting effects demonstrated vitro vivo systems....
The effect of different wine antioxidant polyphenols (catechin, epicatechin, quercetin, and resveratrol) on the growth three prostate cancer cell lines (LNCaP, PC3, DU145) was investigated. A dose- time-dependent inhibition by found at nanomolar concentrations. proliferation LNCaP PC3 cells preferentially inhibited flavonoids quercetin), whereas resveratrol most potent inhibitor DU145 growth. Possible mechanisms action were investigated: 1) competition for androgen binding in revealed...
Abstract BCMA (B cell maturation) is a nonglycosylated integral membrane type I protein that preferentially expressed in mature B lymphocytes. Previously, we reported human malignant myeloma line not primarily present on the surface but lies perinuclear structure partially overlaps Golgi apparatus. We now show transiently or stably transfected cells, located surface, as well perinulear Golgi-like structure. also overexpression of 293 cells activates NF-κB, Elk-1, c-Jun N-terminal kinase, and...
Recent findings have shown that, in addition to the genomic action of steroids, through intracellular receptors, short-time effects could be mediated binding membrane sites. In present study prostate cancer LNCaP cells, we report that dihydrotestosterone and non-internalizable analog testosterone-BSA increase rapidly release prostate-specific antigen (PSA) culture medium. Membrane testosterone sites were identified ligand on preparations, flow cytometry, confocal laser microscopy fluorescent...
The BCMA gene is a new discovered by the molecular analysis of t(4;16) translocation, characteristic human T cell lymphoma. It has no significant similarity with any known protein or motif, so that its function was unknown. This report describes cloning murine cDNA and genomic counterpart. mouse organized into three exons, like gene, lies in chromosome 16, 16B3 band, counterpart 16p13 where lies. Murine encodes 185 amino acids (184 residues for human), potential central transmembrane segment...
The Ras family GTPases RalA and RalB have been defined as central components of the regulatory machinery supporting tumor initiation progression. Although it is known that Ral proteins mediate oncogenic signaling physically functionally interact with vesicle trafficking machinery, their mechanistic contribution to transformation unknown. Here, we directly evaluated relative effector pathways cell motility directional migration. Through loss-of-function analysis, find not limiting for...
Nongenomic androgen actions imply mechanisms different from the classical intracellular receptor (iAR) activation. We have recently reported identification of a membrane (mAR) on LNCaP human prostate cancer cells, mediating testosterone signal transduction within minutes. In present study we provide evidence that activation mAR by nonpermeable, BSA-coupled results in 1) inhibition cell growth (with 50% inhibitory concentration 5.08 nM, similar to affinity for sites); 2) induction cells both...
The neuroprotective role of estrogen (E2) is supported by a multitude experimental and epidemiological data, although its mode action not fully understood. present work was conducted to study the underlying mechanisms action, using rat cell line PC12, an established model for neuronal apoptosis survival. Our results show that E2 (but androgens or progestins) prevent growth inhibition PC12 cells, induced serum deprivation. Several were investigated: 1) intracellular receptors (ERs) have been...
Targeting the signaling lipid S1P might suppress tumor growth by slowing mitosis.
A new casomorphin pentapeptide (αS1-casomorphin) has been isolated from the sequence of human αS1-casein [αS1-casein-(158–162)], with Tyr-Val-Pro-Phe-Pro. This peptide was found to bind high affinity all three subtypes κ-opioid receptor (κ1–κ3). When amidated at C-terminus, αS1-casomorphin amide binds Δ- and κ3-opioid sites. Both its inhibit in a dose-dependent reversible manner proliferation T47D breast cancer cells. anti-proliferative activity greater for αS1-casomorphin, which most potent...
Abstract Genomic signaling mechanisms require a relatively long time to get into action and represent the main way through which steroid hormones affect target cells. In addition, steroids may rapidly activate cellular functions by non-genomic involving membrane sites. Understanding in depth molecular of represents an important frontier for developing new more selective pharmacologic tools endocrine therapies. present study, we report that membrane-impermeable testosterone-bovine serum...
Gem is a protein of the Ras superfamily that plays role in regulating voltage-gated Ca2+ channels and cytoskeletal reorganization. We now report GTP-bound interacts with membrane-cytoskeleton linker Ezrin its active state, binds to cells. The coexpression induces cell elongation accompanied by disappearance actin stress fibers collapse most focal adhesions. same morphological effect elicited when cells expressing alone are stimulated serum requires expression ERM proteins. show endogenous...
Opioids and somatostatin analogs have been implicated in the modulation of renal water handling, but whether their action is accomplished through central and/or peripheral mechanisms remains controversial. In different cell systems, on other hand, opioids inhibit proliferation. present study, we used an established line, derived from opossum kidney (OK) proximal tubules, order to characterize opioid receptors investigate tubular epithelial tissue. Our results show presence one class binding...
Opioids and somatostatin analogs have been implicated in the modulation of renal water handling, but whether their action is accomplished through central and/or peripheral mechanisms remains controversial. In different cell systems, on other hand, opioids inhibit proliferation. present study, we used an established line, derived from opossum kidney (OK) proximal tubules, order to characterize opioid receptors investigate tubular epithelial tissue. Our results show presence one class binding...
Opioids decrease cell proliferation in different systems including breast, prostate, lung, kidney, and intestine, through an interaction with opioid as well other membrane-receptor (somatostatin, cholinergic), unidentified mechanism. Recently, we have reported of taxol membrane sites (BBRC 235, 201–204, 1997), involvement opioids to the modification actin cytoskeleton renal OK cells (J Cell Biochem. [1998] 70:60–69), indicating a possible action effect. In present work, examined effect two...