A5055249346- Neural dynamics and brain function
- Memory and Neural Mechanisms
- Neuroscience and Neuropharmacology Research
- Neuroscience and Neural Engineering
- Advanced Memory and Neural Computing
- EEG and Brain-Computer Interfaces
- Sleep and Wakefulness Research
- Neural Networks and Applications
- Stress Responses and Cortisol
- Zebrafish Biomedical Research Applications
- Photoreceptor and optogenetics research
- Receptor Mechanisms and Signaling
- Olfactory and Sensory Function Studies
- Memory Processes and Influences
- Ion channel regulation and function
- Spatial Cognition and Navigation
- Functional Brain Connectivity Studies
- Architecture and Computational Design
- Visual perception and processing mechanisms
- Blind Source Separation Techniques
- 3D Printing in Biomedical Research
- Creativity in Education and Neuroscience
- Genetics and Neurodevelopmental Disorders
- Tactile and Sensory Interactions
- Cystic Fibrosis Research Advances
Neuroelectronics Research Flanders
2016-2025
VIB-KU Leuven Center for Brain & Disease Research
2016-2025
KU Leuven
2015-2025
IMEC
2015-2022
VIB-KU Leuven Center for Microbiology
2017-2022
Charité - Universitätsmedizin Berlin
2021
Vlaams Instituut voor Biotechnologie
2021
Institute of Cognitive and Brain Sciences
2009-2013
Massachusetts Institute of Technology
2003-2013
Netherlands Institute for Neuroscience
2002-2005
Measuring the dynamics of neural processing across time scales requires following spiking thousands individual neurons over milliseconds and months. To address this need, we introduce Neuropixels 2.0 probe together with newly designed analysis algorithms. The has more than 5000 sites is miniaturized to facilitate chronic implants in small mammals recording during unrestrained behavior. High-quality recordings long were reliably obtained mice rats six laboratories. Improved site density...
Spatial learning requires the hippocampus, and replay of spatial sequences during hippocampal sharp wave-ripple (SPW-R) events quiet wakefulness sleep is believed to play a crucial role. To test whether coordination VTA reward prediction error signals with these replayed could contribute this process, we recorded from neuronal ensembles hippocampus as rats performed appetitive tasks subsequently slept. We found that many responsive (RR) neurons coordinated wakefulness-associated SPW-R recent...
We present a high electrode density and channel count CMOS (complementary metal-oxide-semiconductor) active neural probe containing 1344 neuron sized recording pixels (20 µm × 20 µm) 12 reference 80 µm), densely packed on 50 thick, 100 wide, 8 mm long shank. The electrodes or consist of dedicated in-situ circuits for signal source amplification, which are directly located under each electrode. supports the simultaneous all 1356 with sufficient to noise ratio typical neuroscience...
A fundamental task in neuroscience is to understand how neural ensembles represent information. Population decoding a useful tool extract information from neuronal populations based on the ensemble spiking activity. We propose novel Bayesian paradigm decode unsorted spikes rat hippocampus. Our approach uses direct mapping between spike waveform features and covariates of interest avoids accumulation sorting errors. nonparametric, encoding model-free for representing stimuli, extracts all...
The tetrode, a bundle of four electrodes, has proven to be valuable tool for the simultaneous recording multiple neurons in-vivo. differential amplitude action potential signatures over channels tetrode allows isolation single-unit activity from multi-unit signals. ability precisely control stereotaxic location and depth is critical studying coordinated neural across brain regions. In combination with micro-drive array, it possible achieve precise placement stable many tetrodes course days...
Chronic recording of large populations neurons is a valuable technique for studying the function neuronal circuits in awake behaving rats. Lightweight devices carrying high density array tetrodes allow simultaneous monitoring activity tens to hundreds individual neurons. Here we describe protocol fabrication micro-drive with twenty one independently movable micro-drives. This device has been used successfully record from hippocampal and cortical our lab. We show how prepare custom designed,...
Abstract In this study, we analyzed in detail the topographic organization of subiculoparahippocampal projection rat. The anterograde tracers Phaseolus vulgaris leucoagglutinin‐L and biotinylated dextran amine were injected into subiculum at different septotemporal transverse levels. Deep layers ento‐, peri‐, postrhinal cortices are main recipients subicular projections, but all cases noted that a small fraction projections also terminates superficial II III. Analysis fiber patterns...
Abstract The hippocampal formation communicates with the neocortex mainly through adjacent entorhinal cortex. Neurons projecting to are found in superficial layers of cortex and largely segregated from neurons receiving output, which located deep layers. We studied communication between anaesthetized rat using field potential recordings, current source density analysis single unit measurements. that subiculum stimulation was able excite This response followed by sinks Both responses were...
See Lenck-Santini (doi:10.1093/awx205) for a scientific commentary on this article. Epileptic seizures represent altered neuronal network dynamics, but the temporal evolution and cellular substrates of activity patterns associated with spontaneous are not fully understood. We used simultaneous recordings from multiple neurons in hippocampus neocortex rats chronic lobe epilepsy to demonstrate that subsets cells discharge highly stereotypical sequential pattern during ictal events, these were...
Neuropixels probes contain thousands of electrodes across one or more shanks and are sufficiently small to allow chronic recording neural activity in freely behaving animals. However, the joint increase number miniaturization probe package has led a compromise which groups share single read-out channel only fraction can be read out at any given time. Experimenters then face challenge selecting subset (i.e., map) that both covers brain regions interest adheres restrictions underlying...
The hippocampus is known to encode spatial information and reactivate experienced trajectories during sharp-wave ripple events. These events are thought be key time-points at which about learned transferred the neocortex for long-term storage. It unclear, however, how this may integrated in downstream cortical regions. In study, we performed high-density probe recordings across full depth of medial prefrontal cortex simultaneously rats while they were performing a task navigation. We find...
The hippocampus is known to encode spatial information and reactivate experienced trajectories during sharp-wave ripple events. These events are thought be key time-points at which about learned transferred the neocortex for long-term storage. It unclear, however, how this may integrated in downstream cortical regions. In study, we performed high-density probe recordings across full depth of medial prefrontal cortex simultaneously rats while they were performing a task navigation. We find...
There is controversy concerning whether orthodromic action potentials originate from the apical or basal dendrites of CA1 pyramidal cells in vivo. The participation initialization and propagation population spikes urethan-anesthetized rats vivo was studied using simultaneously recorded field current source density (CSD) analysis. CSD analysis revealed that antidromic spike, evoked by stimulation alveus, invaded succession, axon initial segment (stratum oriens), cell body ∼200 μm proximal...
The entorhinal cortex has long been recognized as an important interface between the hippocampal formation and neocortex. notion of bidirectional connections have led to suggestion that output originating in CA1 subiculum may reenter subfields via cortex. To investigate this, we used simultaneous multi-site field potential recordings current source density analysis rat vivo. Under ketamine/xylazine anesthesia, found repetitive stimulation or Schaffer collaterals facilitated responses, such a...
Understanding how neuronal assemblies underlie cognitive function is a fundamental question in system neuroscience. It poses the technical challenge to monitor activity of populations neurons, potentially widely separated, relation behaviour. In this paper, we present new which aims at simultaneously recording from large population neurons multiple separated brain regions freely behaving animals.The concept device combine benefits two existing electrophysiological techniques, i.e....
We present a high density CMOS neural probe with active electrodes (pixels), consisting of dedicated in-situ circuits for signal source amplification. The complete contains 1356 neuron sized (20×20 μm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> ) pixels densely packed on 50 thick, 100 wide and 8 mm long shank. It allows simultaneous high-performance recording from 678 possibility to simultaneously observe all the increased noise....
Closed-loop experiments provide unique insights into brain dynamics and function. To facilitate a wide range of closed-loop experiments, we created an open-source software platform that enables high-performance real-time processing streaming experimental data.We wrote Falcon, C++ multi-threaded in which the user can load execute arbitrary graph. Each node Falcon graph is mapped to single thread nodes communicate with each other through thread-safe buffers. The framework allows for easy...
Communication in neural circuits across the cortex is thought to be mediated by spontaneous temporally organized patterns of population activity lasting ~50 -200 ms. Closed-loop manipulations have unique power reveal direct and causal links between such their contribution cognition. Current brain-computer interfaces, however, are not designed interpret multi-neuronal spiking at millisecond timescale. To bridge this gap, we developed a system for classifying ensemble closed-loop setting...
Previous studies have highlighted an important role for hippocampal sharp-wave ripples in spatial alternation learning, as well modulating activity the medial prefrontal cortex (mPFC). However, direct influence of on mPFC during learning has not been investigated. Here, we train Long Evans rats a three-arm radial maze to perform sequence alternations. Three sequences needed be learned, and while sequence, was inhibited either directly following hippocampus (on-time condition) or with...
Storing and accessing memories is required to successfully perform day-to-day tasks, for example engaging in a meaningful conversation. Previous studies both rodents primates have correlated hippocampal cellular activity with behavioral expression of memory. A key role has been attributed awake replay - sequential reactivation neurons representing trajectory through space. However, it unclear if impacts immediate future behavior, gradually creates stabilizes long-term over long period time...