A5083643337- Computational Drug Discovery Methods
- Synthesis and biological activity
- Analytical Chemistry and Chromatography
- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Cholinesterase and Neurodegenerative Diseases
- Food Quality and Safety Studies
- Neuroscience and Neuropharmacology Research
- Malaria Research and Control
- Free Radicals and Antioxidants
- Eicosanoids and Hypertension Pharmacology
- Antibiotic Resistance in Bacteria
- Tryptophan and brain disorders
- Synthesis and Characterization of Heterocyclic Compounds
- Fatty Acid Research and Health
- Microfluidic and Capillary Electrophoresis Applications
- Biopolymer Synthesis and Applications
- Pharmacological Effects of Natural Compounds
- Advanced biosensing and bioanalysis techniques
- Chromatography in Natural Products
- Antibiotics Pharmacokinetics and Efficacy
- Fermentation and Sensory Analysis
- Inflammatory mediators and NSAID effects
- Tea Polyphenols and Effects
- Alzheimer's disease research and treatments
Comenius University Bratislava
2019-2024
Institute of Neuroimmunology of the Slovak Academy of Sciences
2022-2024
Slovak Academy of Sciences
2022-2024
University of Ss. Cyril and Methodius in Trnava
2019-2022
Lipids represent a large group of biomolecules that are responsible for various functions in organisms. Diseases such as diabetes, chronic inflammation, neurological disorders, or neurodegenerative and cardiovascular diseases can be caused by lipid imbalance. Due to the different stereochemical properties composition fatty acyl groups molecules most classes, quantification lipids development lipidomic analytical techniques problematic. Identification species from complex matrices is...
POM analysis and related approaches are significant tools based on calculating various physico-chemical properties predicting biological activity, ADME parameters, toxicity of a molecule. These methods used to evaluate molecule's potential become drug candidate. Avenanthramides (AVNs) promising secondary metabolites specific Avena spp. (oat). They comprise the amides anthranilic acid linked polyphenolic acids with or without post-condensation molecule transformation. natural compounds have...
A library of novel 4-{[(benzyloxy)carbonyl]amino}-2-hydroxybenzoic acid amides was designed and synthesized in order to provide potential acetyl- butyrylcholinesterase (AChE/BChE) inhibitors; the vitro inhibitory profile selectivity index were specified. Benzyl(3-hydroxy-4-{[2-(trifluoromethoxy)phenyl]carbamoyl}phenyl)carbamate best AChE inhibitor with concentration IC50 = 36.05 µM series, while benzyl{3-hydroxy-4-[(2-methoxyphenyl)carbamoyl]phenyl}-carbamate most potent BChE (IC50 22.23 µM)...
Nowadays, lipidomics plays a crucial role in the investigation of novel biomarkers various diseases. Its implementation into field clinical analysis led to identification specific lipids and/or significant changes their plasma levels patients suffering from cancer, Alzheimer’s disease, sepsis, and many other diseases pathological conditions. Profiling determination concentrations could also be helpful case drug therapy management, especially combination with therapeutic monitoring (TDM)....
A series of nineteen novel ring-substituted N-arylcinnamanilides was synthesized and characterized. All investigated compounds were tested against Staphylococcus aureus as the reference strain, two clinical isolates methicillin-resistant S. (MRSA), Mycobacterium tuberculosis. (2E)-N-[3-Fluoro-4-(trifluoromethyl)phenyl]-3-phenylprop-2-enamide showed even better activity (minimum inhibitory concentration (MIC) 25.9 12.9 µM) MRSA than commonly used ampicillin (MIC 45.8 µM). The screening cell...
Many biologically active metabolites of the essential amino acid L-tryptophan (Trp) are associated with different neurodegenerative diseases and neurological disorders. Precise reliable methods for their determination needed. Variability in physicochemical properties makes analytical process challenging. In this case, chemical modification analyte derivatization could come into play. Here, we introduce a novel fast reversed-phase ultra-high-performance liquid chromatography (RP-UHPLC)...
Due to the urgent need of innovation in antimalarial therapeutic arsenal, a series thirty-seven ring-substituted N-arylcinnamanilides prepared by microwave-assisted synthesis were subjected primary screening against chloroquine-sensitive strain P. falciparum 3D7/MRA-102. The lipophilicity all compounds was experimentally determined as logarithm capacity factor k, and these data subsequently used discussion structure-activity relationships. Among screened compounds, fourteen derivatives...
Abstract Methylxanthines such as caffeine, theophylline, theobromine are significant and widespread psychoactive substances. We developed the isocratic method with optimum composition of mobile phase 90 % water: 10 acetonitrile confirmed repeatability retention times peak areas. The HPLC was applied to determine content methylxanthines in selected types black green teas available on market. Of (tea bags), highest concentration found Ceylon tea (18.98 mg.L −1 ). caffeine a cup Earl Gray...
Biogenic amines are low-molecular-weight nitrogen compounds that formed primarily by the decarboxylation of amino acids microbial enzymes. These active substances found mainly in various types food or beverages. At certain concentrations, biogenic essential for many physiological functions but toxic if consumed large quantities. Therefore, development and optimization methods sensitive to determine these very much needed. The determination beverages most commonly used separation with...
A series of six di- and tri-halogenated N-arylcinnamanilides designed as anti-inflammatory antimicrobial agents was prepared characterized. Since it is known that lipophilicity significantly influences the biological activity compounds, hydro-lipophilic properties these tri-substituted were investigated in study. All discussed derivatives cinnamic acid analyzed using reversed-phase high performance liquid chromatography method to measure lipophilicity. The procedure performed under isocratic...
Alzheimer's disease (AD) is a multifactorial neurological of unknown etiology that associated with various risk factors. Various pharmacological approaches targeting distinct mechanisms have been investigated; however, they not yet achieved disease-modifying effects. A series nine trisubstituted 1,3,5-triazine-based derivatives was investigated as potential inhibitors the β-secretase enzyme (beta-site amyloid precursor protein-cleaving 1, BACE1), one key enzymes in pathogenesis AD. Although...
A series of seven fluorinated benzyl carbamates 4-aminosalicylanilides and unsubstituted [3-hydroxy-4-(phenylcarbamoyl)phenyl]carbamate designed as agents with the expected anticholinesterase anti-inflammatory activity were prepared characterized. As lipophilicity significantly influences biological compounds, hydro-lipophilic properties these mono-, di-, tri-substituted investigated in this study. All discussed derivatives 4-{[(benzyloxy)carbonyl]amino}-2-hydroxybenzoic acid analyzed using...
Phenolic amides contained in oats (Avenanthramides; AVNs) are biologically active substances with strong antioxidant activity. In this paper, we evaluated efficiency of three C18 chromatographic columns (Symmetry, XBridge, Cortecs) different particle technology and sizes for the separation major avenanthramides (AVN A, AVN B, C). We compared terms retention times, factors AVNs other parameters such as number theoretical plates, height equivalent to a plate, reduced plate height, resolution...
Recently, a series of thirty-eight 4-{[(benzyloxy)carbonyl]amino}-2-hydroxybenzoic acid amides designed as potential acetyl- and butyrylcholinesterase (AChE/BChE) inhibitors were described drugs to alleviate the symptoms Alzheimer's disease (AD). Some these compounds have shown promise for inhibiting either AChE or BChE. Since are structurally similar agents beta-site amyloid precursor protein cleaving enzyme 1 (BACE1), aim contribution was verify how our able affect this enzyme, which, when...