A5054051531- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- SARS-CoV-2 and COVID-19 Research
- interferon and immune responses
- Influenza Virus Research Studies
- COVID-19 Clinical Research Studies
- Antimicrobial Peptides and Activities
- Animal Virus Infections Studies
- T-cell and B-cell Immunology
Boston Children's Hospital
2021-2024
Harvard University
2024
Global deployment of vaccines that can provide protection across several age groups is still urgently needed to end the COVID-19 pandemic, especially in low- and middle-income countries. Although against SARS-CoV-2 based on mRNA adenoviral vector technologies have been rapidly developed, additional practical scalable are required meet global demand. Protein subunit formulated with appropriate adjuvants represent an approach address this urgent need. The receptor binding domain (RBD) a key...
Vaccination can help prevent infection and also be used to treat cancer, allergy, potentially even drug overdose. Adjuvants enhance vaccine responses, but currently, the path their advancement development is incremental. We a phenotypic small-molecule screen using THP-1 cells identify nuclear factor-κB (NF-κB)-activating molecules followed by counterscreening lead target libraries with quantitative tumor necrosis factor immunoassay primary human peripheral blood mononuclear cells. Screening...
Adjuvanted nanocarrier-based vaccines hold substantial potential for applications in novel early-life immunization strategies. Here, via mouse and human age-specific vitro modeling, we identified the combination of a small-molecule STING agonist (2′3′-cyclic GMP-AMP, cGAMP) TLR7/8 (CL075) to drive synergistic activation neonatal dendritic cells precision CD4 T-helper (Th) cell expansion IL-12/IFNγ axis. We further demonstrate that vaccination mice with quadrivalent influenza recombinant...
Global deployment of vaccines that can provide protection across several age groups is still urgently needed to end the COVID-19 pandemic especially for low- and middle-income countries. While against SARS-CoV-2 based on mRNA adenoviral-vector technologies have been rapidly developed, additional practical scalable are meet global demand. In this context, protein subunit formulated with appropriate adjuvants represent a promising approach address urgent need. Receptor-binding domain (RBD) key...
Vaccines are a key biomedical intervention to prevent the spread of infectious diseases, but their efficacy can be limited by insufficient immunogenicity and nonuniform reactogenic profiles. Adjuvants molecules that potentiate vaccine responses inducing innate immune activation. However, there number adjuvants in approved vaccines, current approaches for preclinical adjuvant discovery development inefficient. To enhance identification, we developed protocol based on
ABSTRACT Adjuvanted nanocarrier-based vaccines hold substantial potential for applications in novel early-life immunization strategies. Here, via mouse and human age-specific vitro modelling, we identified the combination of a small molecule STING agonist (2′3′-cyclic GMP-AMP, cGAMP) TLR7/8 (CL075) to drive synergistic activation neonatal dendritic cells precision CD4 Th cell expansion IL-12/IFNγ axis. We further demonstrate that vaccination mice with quadrivalent influenza recombinant...