A5053676101- X-ray Diffraction in Crystallography
- Lanthanide and Transition Metal Complexes
- Crystallization and Solubility Studies
- Monoclonal and Polyclonal Antibodies Research
- Microtubule and mitosis dynamics
- Metal complexes synthesis and properties
- Advanced biosensing and bioanalysis techniques
- Genomics, phytochemicals, and oxidative stress
- Molecular Sensors and Ion Detection
- Cell Adhesion Molecules Research
- Cancer therapeutics and mechanisms
- Cancer-related Molecular Pathways
- Ubiquitin and proteasome pathways
- RNA Research and Splicing
- Nanoplatforms for cancer theranostics
- Electron Spin Resonance Studies
- Cellular Mechanics and Interactions
- Melanoma and MAPK Pathways
- Luminescence Properties of Advanced Materials
- Nanocomposite Films for Food Packaging
- DNA and Nucleic Acid Chemistry
- 14-3-3 protein interactions
- Luminescence and Fluorescent Materials
- Synthesis and Biological Evaluation
- 3D Printing in Biomedical Research
École Pratique des Hautes Études
2015-2025
Université Grenoble Alpes
2015-2025
Inserm
2016-2025
Centre National de la Recherche Scientifique
2015-2025
Université Paris Sciences et Lettres
2016-2025
Institut de Biosciences et Biotechnologies
2017-2024
Institut pour l'avancée des biosciences
1997-2024
RMIT University
2023
Agir Pour les Maladies Chroniques
2021
Université Joseph Fourier
1995-2015
Despite advances in the development of bone substitutes and strict aseptic procedures, majority failures grafting surgery are related to nosocomial infections. Development biomaterials combining both osteogenic antibiotic activity is, therefore, a crucial public health issue. Herein, two types intrinsically bactericidal titanium supports were fabricated by using commercially scalable techniques: plasma etching or hydrothermal treatment, which display separate mechanisms mechano-bactericidal...
ABCG2 plays a major role in anticancer-drug efflux and related tumor multidrug resistance. Potent selective inhibitors with low cytotoxicity were investigated among series of 44 chalcones analogues (1,3-diarylpropenones), by evaluating their inhibitory effect on the transport mitoxantrone, known substrate. Six compounds producing complete inhibition IC(50) values below 0.5 μM high selectivity for identified. The number position methoxy substituents appeared to be critical both cytotoxicity....
ABSTRACT The role of talin was addressed by down regulating its expression using an antisense RNA strategy. HeLa cells were transfected with a 5′ cDNA fragment under the control inducible human metallothionein promotor. Isolated clones displayed decrease in level to 10% control. reduction dramatically slowed kinetics cell spreading. Mocktransfected cells, spread out onto fibronectin, exhibited large peripheral adhesion plaques. In contrast, reduced showed smaller focal contacts localized all...
Lanthanide(III) complexes with two-photon absorbing antennas are attractive for microscopy imaging of live cells because they can be excited in the NIR. We describe synthesis and luminescence properties two Eu3+ complexes, mTAT[Eu·L-CC-Ar–Cz] mTAT[Eu·L-Ar–Cz], (N-carbazolyl)-aryl-alkynyl-picolinamide (N-carbazolyl)-aryl-picolinamide antennas, respectively, conjugated to TAT cell-penetrating peptides. Contrary what was previously observed related carbazole-based a mixture water organic...
Luminescent two-photon (2P) absorbing lanthanide(III) complexes hold great promise for microsocpy imaging of biological samples. Conjugating such a complex to some cell penetrating peptides (CPP) allows its controlled delivery to...
Dietary flavonoids have been shown to exert specific cytotoxicity toward some cancer cells, but the precise molecular mechanisms are still not completely understood. In this study, cytotoxic effects of flavones (apigenin and luteolin) on two different cell lines, including human chronic myelogenous erythroleukaemia (K562) bladder carcinoma (RT112), were determined, responsible for their studied. The results an MTT assay showed that luteolin apigenin able induce in K562 RT112 cells a dose-...
We previously identified 1-(2,4-dimethoxyphenyl)-3-(1-methylindolyl) propenone (IPP51), a new chalcone derivative that is capable of inducing prometaphase arrest and subsequent apoptosis bladder cancer cells. Here, we demonstrate IPP51 selectively inhibits proliferation tumor-derived cells versus normal non-tumor interfered with spindle formation mitotic chromosome alignment. Accumulation cyclin B1 checkpoint proteins Bub1 BubR1 on chromosomes in treated indicated the activation...
We report a family of luminescent lanthanide bioprobes featuring two-photon absorbing antenna and dimer the TAT cell penetrating peptides. They penetrate live cells efficiently allow microscopy with commercial microscopes.
Lanthanide(III) (Ln3+) complexes feature desirable luminescence properties for cell microscopy imaging, but cytosolic delivery of Ln3+ and their use 2P imaging live cells are challenging. In this article, we describe the synthesis spectroscopic characterizations a series based on two ligands, L1 L2, featuring extended picolinate push-pull antennas longer wavelength absorption as well free carboxylate function conjugation to peptides. Several penetrating peptide/Ln3+ complex conjugates were...
Talin is a major cytosolic protein that links the intracellular domains of beta1 and beta3 integrins to cytoskeleton. It required for focal adhesion assembly. However, its downregulation not only slows down cell spreading organization adhesions but also impairs maturation some integrins, including fibronectin receptor alpha5beta1. To investigate this, we characterized integrin synthesized in cells expressing talin anti-sense RNA (AT22 cells). We identified large pool abnormally accumulated...
Copper(II) complexes 12+–6 of a series tripodal ligands involving N3O donor set, namely 2-[(bis-pyridin-2-ylmethyl-amino)-methyl]-4-methoxy-phenol (1L), 2-tert-butyl-4-methoxy-6-[bis-pyridin-2-ylmethyl-amino)-methyl]-phenol (2L), 2-tert-butyl-4-methoxy-6-{[(2-pyridin-2-yl-ethyl)-pyridin-2-ylmethyl-amino]-methyl}-phenol (3L), 2-tert-butyl-4-methoxy-6-{[(6-methyl-pyridin-2-ylmethyl)-pyridin-2-ylmethyl-amino]-methyl}-phenol (4L),...
ABSTRACT With the exception of divergent β4 and β8 chains, integrin β subunit cytoplasmic domains are short highly conserved sequences. Consensus motifs found among different chains. Experiments using chimeric receptors demonstrated that 47 amino acids β1 domain contain sufficient information to target integrins adhesion plaques. Three clusters acids, named cyto-1, cyto-2 cyto-3, seem contribute this localization. Cyto-2 cyto-3 exhibit NPXY motifs. At present, exact function these remains...
Despite the emergence of targeted therapies and immunotherapy, chemotherapy remains gold-standard for treatment most patients with solid malignancies. Spindle poisons that interfere microtubule dynamics are commonly used in drug combinations. However, their troublesome side effects chemoresistance highlight need identifying alternative agents. We performed a high throughput cell-based screening selected pyrrolopyrimidine molecule (named PP-13). In present study, we evaluated its anticancer...
The CD20 antigen is a key target for several diseases including lymphoma and autoimmune diseases. For over 20 years, monoclonal antibodies were developed to treat CD20-related disorders. As many therapeutic proteins, their clinical use however limited due nature with costly biotechnological procedure side effects such as the production of anti-drug neutralizing antibodies. Nucleic acid aptamers have some advantages mAbs are currently investigated use. We herein report selection DNA aptamer...
Propolis is a byproduct widely used in folk medicine due to its valuable biological and pharmacological properties. In this study, vitro antioxidant, antimicrobial antiproliferative capacities of propolis ethanolic extracts (EEPs) collected from four Tunisian regions were evaluated. EEPs showed high total antioxidant antiradical active against all tested microorganisms. These correlated the amounts phenolics samples. Cytotoxicity on human bladder cancer cells RT112 was assessed using both...
1-(2,4-dimethoxyphenyl)-3-(1-methylindolyl) propenone, namely IPP51, was identified by screening a library of 3-indolyl-1-phenylpropenones. IPP51 investigated for its ability to inhibit proliferation and/or induce apoptosis human bladder cancer cell lines and assess potential use in carcinoma treatment. After treating the cells with 24 h, title compound induced predominant reversible G2+M accumulation at prometaphase stage mitosis. However, when used longer period, it leads apoptosis. These...
<b><i>Background:</i></b> The activation of Fas/Fas ligand (FasL) and DR4-DR5/tumor necrosis factor-related-apoptosis-inducing (TRAIL) pathways in cancer cells triggers apoptosis. objective this study was to investigate the prognostic value soluble FasL (sFasL) (sTRAIL) serum patients with bladder cancer. <b><i>Methods:</i></b> sFasL sTRAIL levels sera or healthy donors were determined using enzyme-linked immunosorbent assay. Micro-culture...