Cell refractive index, an intrinsic optical parameter, is closely correlated with the intracellular mass and concentration. By combining phase-shifting interferometry (PSI) atomic force microscope (AFM) imaging, we constructed a label free, non-invasive quantitative index of single cell measurement system, in which accurate phase map was retrieved PSI technique morphology nanoscale resolution achieved AFM imaging. Based on proposed AFM/PSI distributions red blood Jurkat cell, respectively....

Using the maximum and minimum of interference, a novel two-step phase demodulation algorithm is proposed to perform extraction in phase-shifting interferometry. By means simulation calculation experimental research, it proved that both measured shift with high precision can be obtained algorithm.

Abstract Incoherent digital holography no longer requires spatial coherence of the light field, breaking through imaging resolution coherent holography. However, traditional reconstruction methods cannot avoid inherent contradiction between temporal and signal‐to‐noise ratio, which is mitigated by deep learning methods, there are problems such as dataset labeling insufficient generalization ability. Here, a self‐calibrating approach with an untrained network proposed fusing plug‐and‐play...

Cardiac fibrosis is a pathological process characterized by excess extracellular matrix (ECM) deposition and plays critical role in nearly all types of heart disease. The mechanism cardiac still unclear no effective medication treatment fibrosis. Research showed that mesenchymal stem cell (MSC) derived exosomes may play effect human amniotic fluid MSC (hAFMSC)-derived (hAFMSCExos) on has remained unclear.The hAFMSCExos were extracted using sequential centrifugation approach. effects...

Fresnel incoherent correlation holography (FINCH) realizes non-scanning three-dimension (3D) images using spatial illumination, but it requires phase-shifting technology to remove the disturbance of DC term and twin that appears in reconstruction field, thus increasing complexity experiment limits real-time performance FINCH. Here, we propose a single-shot via deep learning based (FINCH/DLPS) method realize rapid high-precision image only collected interferogram. A network is designed...

Abstract Spontaneous coronary artery dissection is a non-atherosclerotic cause of acute syndrome, particularly among young women. A 46-year-old female without family history cardiac disease and traditional atherosclerotic risk factors presented to hospital with non-ST-segment elevation myocardial infarction. Diagnostic angiography revealed moderate stenosis the left main anterior descending blood flow thrombolysis in infarction grade 3. The circumflex was observed hypoplastic significant...

ABSTRACT Objective The study evaluates the efficacy of 18 F‐2‐fluoro‐2‐deoxy‐d‐glucose FDG PET/CT in predicting outcomes patients with advanced head and neck squamous cell carcinoma receiving neoadjuvant chemoimmunotherapy. Methods In this prospective study, we compared parameters 63 LA‐HNSCC who underwent F‐FDG before chemoimmunotherapy, divided into responders (47) non‐responders (16), after three cycles ratio maximum standardized uptake value (SUVmax) primary tumor to SUVmean normal...

In this Letter, the character of Euclidean matrix norm (EMN) intensity difference between phase-shifting interferograms, which changes in sinusoidal form with phase shifts, is presented. Based on character, an EMN shift extraction algorithm proposed. Both simulation calculation and experimental research show that shifts high precision can be determined proposed easily. Importantly, will supply a powerful tool for rapid calibration shifts.

From three interferograms with unknown phase shifts, an innovative retrieval approach based on the normalized difference maps is proposed. Using subtraction operation between interferograms, two without background can be achieved. To eliminate amplitude inequality of maps, normalization process employed so that are obtained. Finally, combining and two-step algorithm, measured high precision retrieved rapidly. Comparing conventional algorithm high-pass filtering, accuracy processing time...

A simultaneous phase-shifting dual-wavelength interferometry based on two-step demodulation algorithm is proposed in this Letter. First, two lasers with different wavelengths go through the same inline interference system simultaneously, and a sequence of five frames interferograms (SPSDWIs) special phase shifts are captured by monochrome CCD. Subsequently, using subtraction between first SPSDWI other SPSDWI, each wavelength single-wavelength images (SWIIs) without background can be...

Fresnel incoherent correlation holography (FINCH) enables high-resolution 3D imaging of objects from several 2D holograms under light and has many attractive applications in motionless fluorescence imaging. However, FINCH difficulty implementing dynamic scenes since multiple phase-shifting need to be recorded for removing the bias term twin image reconstructed scene, which requires object remain static during this progress. Here, we propose a dual-channel noncoherent compressive method....

Direct molecular imaging of nano-spatial relationship between T cell receptor (TCR)/CD3 and CD4 or CD8 co-receptor before after activation a primary has not been reported. We have recently innovated application near-field scanning optical microscopy (NSOM) immune-labeling quantum dots (QD) to image Ag-specific TCR response during in vivo clonal expansion, now up-graded the NSOM/QD-based nanotechnology through dipole-polarization dual-color imaging. Using this system cell-membrane molecules...

In dual-wavelength interferometry (DWI), by combing the advantage of shorter synthetic-wavelength and immune algorithm phase ambiguity, we propose an improved retrieval method with both high accuracy large measurement range, which is a pair contradiction in reported DWI method. First, calculate height measured object at longer through using wrapped phases two single-wavelengths. Second, combining ambiguity synthetic-wavelength, can perform unwrapping larger one single-wavelengths, then...

Phase-shifting interferometry (PSI) requires accurate phase shifts between interferograms for realizing high-accuracy retrieval. This paper presents an adaptive PSI through synchronously capturing measurement and interferograms, in which the former is a series of spatial carrier frequency phase-shifting generated by additional assembly are calculated with single-spectrum algorithm (SS-PSMA), latter employed retrieval digital holography (PSDHA) based on complex amplitude recovery. In addition...