Cell refractive index, an intrinsic optical parameter, is closely correlated with the intracellular mass and concentration. By combining phase-shifting interferometry (PSI) atomic force microscope (AFM) imaging, we constructed a label free, non-invasive quantitative index of single cell measurement system, in which accurate phase map was retrieved PSI technique morphology nanoscale resolution achieved AFM imaging. Based on proposed AFM/PSI distributions red blood Jurkat cell, respectively....

Abstract Incoherent digital holography no longer requires spatial coherence of the light field, breaking through imaging resolution coherent holography. However, traditional reconstruction methods cannot avoid inherent contradiction between temporal and signal‐to‐noise ratio, which is mitigated by deep learning methods, there are problems such as dataset labeling insufficient generalization ability. Here, a self‐calibrating approach with an untrained network proposed fusing plug‐and‐play...

In dual-wavelength interferometry (DWI), by combing the advantage of shorter synthetic-wavelength and immune algorithm phase ambiguity, we propose an improved retrieval method with both high accuracy large measurement range, which is a pair contradiction in reported DWI method. First, calculate height measured object at longer through using wrapped phases two single-wavelengths. Second, combining ambiguity synthetic-wavelength, can perform unwrapping larger one single-wavelengths, then...

Phase-shifting interferometry (PSI) requires accurate phase shifts between interferograms for realizing high-accuracy retrieval. This paper presents an adaptive PSI through synchronously capturing measurement and interferograms, in which the former is a series of spatial carrier frequency phase-shifting generated by additional assembly are calculated with single-spectrum algorithm (SS-PSMA), latter employed retrieval digital holography (PSDHA) based on complex amplitude recovery. In addition...

Abstract In phase-shifting interferometry with unknown phase shifts, a normalization and orthogonalization algorithm (NOPSA) is proposed to achieve retrieval. The background of interferogram eliminated through using the orthogonality complex sinusoidal function; influence shifts deviation on accuracy retrieval avoided both processing. Compared current algorithms reveals significantly faster computation speed, higher accuracy, better stability non-sensitivity deviation.

Learning-based phase imaging balances high fidelity and speed. However, supervised training requires unmistakable large-scale datasets, which are often hard or impossible to obtain. Here, we propose an architecture for real-time based on physics-enhanced network equivariance (PEPI). The measurement consistency equivariant of physical diffraction images used optimize the parameters invert process from a single pattern. In addition, regularization method total variation kernel (TV-K) function...

Quantitative characterization of intracellular H2O2 content, which is still difficult by the conventional biochemical methods due to lack real-time and non-invasive technique single cell measurement, a useful solution for state assessment. Based on surface enhanced Raman scattering (SERS), we construct novel boric acid (BA) nanoprobe perform quantitative in p-thiol benzene (4-MPBA) reporter molecule modified with gold nanorods (AuNRs) employed signal enhancement. The achieved result...

Both the reactive oxygen species (ROS) level and Phosphatidylinositol 3 Kinase (PI3K) protein content are two crucial parameters for characterizing states of cell apoptosis. Current methods measure these with different techniques, respectively, which usually lead to evaluation contingency. Ginsenoside Rg3 exhibits an excellent anticancer effect, is enacted by Kinase/Protein B (PI3K/Akt) pathway involving ROS; however, precise mechanism that induces apoptosis remains unknown. This due lack...

Background Cerebral microbleeds (CMBs) are an early sign of many neurological disorders and accompanied by local neuroinflammation brain damage. As important regulators immune response neuroinflammation, the biological behavior role γδ T cells after CMBs remain largely unknown. Methods We made a spot injury microvessel in somatosensory cortex to mimic model two-photon laser vivo tracked dynamical behaviors induced using TCR-δ GFP transgenic mice. Biological features peri-CMBs parenchyma were...

A general spatial phase-shifting (GSPS) interferometry method is proposed to achieve phase retrieval from one-frame carrier frequency interferogram. By optimizing the internal signal retrieving function of (SPS) method, accuracy, anti-noise ability and speed can be significantly improved, meanwhile corresponding local calculation property reserved. Especially, in case that ratio variation rate are small, reveals obvious advantage accuracy improvement relative conventional SPS methods, so...

The activation of astrocytes derived from induced pluripotent stem cells (iPSCs) is great significance in neuroscience research, and it crucial to obtain both cellular morphology biomolecular information non-destructively situ, which still complicated by the traditional optical microscopy biochemical methods such as immunofluorescence western blot. In this study, we combined digital holographic (DHM) surface-enhanced Raman scattering (SERS) investigate characteristics iPSCs-derived...

Berberine (BBR), a traditional Chinese herb extract medicine, reveals some anticancer effects in leukemia, but it remains controversial about the molecular mechanism of BBR-induced leukemia cell apoptosis. In this study, combining Raman spectrum and spectral imaging, both biochemical changes Jurkat apoptosis precise distribution BBR single are presented. contrast, we also show corresponding results Jatrorrhizine (JTZ) Palmatine (PMT), two structural analogues BBR. It is found that all three...

Direct molecular imaging of nanoscale relationship between T-cell receptor complexes (TCR/CD3) and gangliosidosis GM1 before after activation has not been reported. In this study, we made use our expertise near-field scanning optical microscopy(NSOM)/immune-labeling quantum dots- (QD-)based dual-color system to visualize profiles for distribution organization TCR/CD3, GM1, as well their nanospatial correlation with PKC θ signaling cascade during activation. Interestingly, anti-CD3/anti-CD28...

Dual-wavelength digital holography effectively expands the measurement range of holography, but it increases complexity optical system due to non-common-path two wavelengths. Here, by using orthogonal polarization strategy, we present a dual-wavelength based on Wollaston prism (DWDH-WP) separate reference beams wavelengths and realize common-path A is inset into beam path off-axis system, so orthogonal-polarized different separated at directions are generated. Then multiplexed interferogram...

From a one-frame off-axis interferogram with unknown carrier-frequency, rapid spatial carrier-frequency phase-shifting (SCFPS) method is proposed to achieve the phase measurement of dynamic process. First, based on SCFPS technique, four-frame subinterferograms are constructed from interferogram. Second, by using orthogonality diamond diagonal vectors, respectively, achieved through subtraction and addition operations between two equal length which generated above subinterferograms, accurate...

A dynamic phase measurement method based on a two-step spatial carrier-frequency phase-shifting (TS-SCFPS) technique is proposed. First, by the subtraction operation between pair interferograms with certain shifts, which are captured dual-channel interferometry system, we can achieve one-frame background-removed interferogram. Second, from two-frame subinterferograms splitting above interferogram, rapidly accurate through using orthogonal and normalization algorithm. Compared existing SCFPS...