A5084608417- Pancreatic function and diabetes
- Diabetes and associated disorders
- Ion Transport and Channel Regulation
- Diabetes Management and Research
- Advanced Biosensing Techniques and Applications
- Optical Coherence Tomography Applications
- Redox biology and oxidative stress
- Photosynthetic Processes and Mechanisms
- Mitochondrial Function and Pathology
- Birth, Development, and Health
- Genetics and Neurodevelopmental Disorders
- Advanced Fluorescence Microscopy Techniques
Saarland University
2023-2025
Abstract Microendoscopy, a crucial technology for minimally invasive investigations of organs, facilitates studies within confined cavities. However, conventional microendoscopy is often limited by probe size and the constraint using single excitation wavelength. In response to these constraints, multichannel microendoscope with slender profile only 360 µm engineered. Functional signals both in situ vivo are successfully captured from individual cells, employing specially developed software...
Abstract The NADPH/NADP + redox couple is central to metabolism and signalling. NADP state differentially regulated by distinct enzymatic machineries at the subcellular compartment level. Nonetheless, a detailed understanding of dynamics limited availability appropriate tools. Here, we introduce NAPstars, family genetically encoded, fluorescent protein-based biosensors. NAPstars offer real-time, specific measurements, across broad-range states, with resolution. NAPstar measurements in yeast,...
Abstract Aims/hypothesis After birth, the neonatal islets gradually acquire glucose-responsive insulin secretion, a process that is subjected to maternal imprinting. Although NEFA are major components of breastmilk and secretagogues, their role for functional maturation beta cells still unclear. endogenous ligands fatty acid receptor 1 (FFA1, encoded by Ffar1 in mice), Gq-coupled with stimulatory effect on secretion. This study investigates FFA1 cell function adaptation offspring parental...
Abstract Microendoscopy enables minimally invasive investigations of organs even within small cavities. Conventional microendoscopy is limited by probe size and often restricted to a single excitation wavelength. We developed characterized multichannel microendoscope as thin 360 µm recorded functional cellular signals in-situ using custom written software for image processing. The endoscope had an effective resolution 4.64 resolved subcellular structures neurons. system enabled analysis...
Abstract Barth syndrome is a multisystem genetic disorder caused by mutation in TAFAZZIN , gene that encodes phospholipid:lysophospholipid transacylase important for cardiolipin remodeling. Syndrome patients suffer from number of symptoms including early heart failure, fatigue, and systemic metabolic alterations, hypoglycemia. The endocrine pancreas central to glucose homeostasis, however, the impact defective remodeling on pancreatic islet function consequences metabolism unclear....
<p> </p> <p>Hypoxia-induced islet cell death, caused by an insufficient revascularization of the grafts, is a major obstacle for successful pancreatic transplantation. Recently, it has been reported that nucleotide-binding oligomerization domain (NOD)-like receptor protein (NLRP)3 inflammasome expressed in islets and its loss protects against hypoxia-induced death. Therefore, we hypothesized inhibition NLRP3 improves survival endocrine function grafts. The transplantation...
<p> </p> <p>Hypoxia-induced islet cell death, caused by an insufficient revascularization of the grafts, is a major obstacle for successful pancreatic transplantation. Recently, it has been reported that nucleotide-binding oligomerization domain (NOD)-like receptor protein (NLRP)3 inflammasome expressed in islets and its loss protects against hypoxia-induced death. Therefore, we hypothesized inhibition NLRP3 improves survival endocrine function grafts. The transplantation...